Recombinant Lectin
Mostrando 1-12 de 64 artigos, teses e dissertações.
-
1. ExpressÃo, purificaÃÃo e caracterizaÃÃo estrutural da bdh-2 recombinante, uma espermadesina presente no plasma seminal de bode (Capra hircus) / Expression, purificaÃÃo and structural characterization of bdh-2 recombinant, a present espermadesina in the plasma seminal of bode (Capra hircus)
O cDNA da bodesina Bdh-2 presente no plasma seminal de caprino (Capra hircus) foi subclonado no vetor de expressÃo pTrcHis TOPO utilizado para transformar cÃlulas E.coli Top 10 One Shot. Os clones recombinantes foram selecionados atravÃs de crescimento em meio LB-Broth contendo 50 μg/mL de ampicilina e amplificaÃÃo do gene por PCR. A sÃntese da pr
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/02/2010
-
2. Impact of galectin-1 on the evolution of acute experimental Trypanosoma cruzi infection / Avaliação da participação da galectina-1 na evolução da infecção experimental aguda por Trypanosoma cruzi
Galectin-1 (Gal-1) is a -galactoside-binding protein and participates in several biological processes, including modulation of immune response. In the literature, there are several reports about the potential therapeutic use of Gal-1 for autoimmune diseases, inflammatory, degenerative and infectious diseases. However, there are few reports on the involvement
Publicado em: 2010
-
3. High levels of serum mannose-binding lectin are associated with the severity of clinical signs of leptospirosis
The clinical heterogeneity observed in leptospirosis may be associated with host factors or bacteria virulence. Human serum mannose-binding lectin (MBL) recognizes many pathogens, and low levels of this lectin are associated with susceptibility to infection. MBL is also implicated in the modulation of the inflammatory process. We determined the levels of ser
Brazilian Journal of Medical and Biological Research. Publicado em: 2009-04
-
4. ProduÃÃo heterÃloga de frutalina em Pichia pastoris / Production heterologous of frutalin in Pichia pastoris
A frutalina, lectina α-D-galactose ligante, foi expressa e processada num sistema heterÃlogo para expressÃo de proteÃnas utilizando a levedura metilotrÃfica Pichia pastoris. Para atingir os objetivos esperados foram desenvolvidas trÃs estratÃgias: identificar o gene que codifica para a frutalina a partir do DNA genÃmico obtido de folhas de A. inc
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/07/2008
-
5. Clonagem e expressÃo de uma lectina de Artocarpus incisa L. (Frutalina) em Escherichia coli / Cloning and expression of a lectin from Artocarpus incisa L. (Frutalina) in Escherichia coli
As lectinas constituem ferramentas biotecnolÃgicas de fundamental importÃncia em estudos histoquÃmicos e citoquÃmicos para a detecÃÃo de glicoconjugados nos tecidos. Entre estas aplicaÃÃes, destacam-se a identificaÃÃo de receptores de membrana e a detecÃÃo de estruturas caracterÃsticas de neoplasias. A Frutalina, uma lectina -D-galactose
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 25/04/2008
-
6. Pulchellis: a patent vegetal toxin ribosome inactivating - type 2 RIP. in vitro and in vivo studies / Pulchellina: uma potente toxina vegetal inativadora de ribossomos - RIP tipo 2. estudos in vitro e in vivo
Pulchellin is a type 2 ribosome-inactivating protein (RIP) isolated from seeds of the Abrus pulchellus tenuiflorus plant. The DNA fiagment encoding Pulchellin A-chain (PAC) was cloned and inserted in pGEX-5X to express the recombinant pulchellin Achain (rPAC) as a fusion protein in Escherichin coli. The deduced amino acid sequence analyses of the rPAC presen
Publicado em: 2005
-
7. Masking and unmasking of the sialic acid-binding lectin activity of CD22 (Siglec-2) on B lymphocytes
CD22 is a B cell-restricted glycoprotein involved in signal transduction and modulation of cellular activation. It is also an I-type lectin (now designated Siglec-2), whose extracellular domain can specifically recognize α2–6-linked sialic acid (Sia) residues. This activity is postulated to mediate intercellular adhesion and/or to act as a coreceptor in a
The National Academy of Sciences.
-
8. The versican C-type lectin domain recognizes the adhesion protein tenascin-R.
The core proteins of large chondroitin sulfate proteoglycans contain a C-type lectin domain. The lectin domain of one of these proteoglycans, versican, was expressed as a recombinant 15-kDa protein and shown to bind to insolubilized fucose and GlcNAc. The lectin domain showed strong binding in a gel blotting assay to a glycoprotein doublet in rat brain extra
-
9. An insecticidal N-acetylglucosamine-specific lectin gene from Griffonia simplicifolia (Leguminosae).
Griffonia simplicifolia II, an N-acetylglucosamine-specific legume lectin, has insecticidal activity when fed to the cowpea weevil, Callosobruchus maculatus (F.). A cDNA clone encoding G. simplicifolia II was isolated from a leaf cDNA library, sequenced, and expressed in a bacterial expression system. The recombinant protein exhibited N-acetylglucosamine-bin
-
10. Oral immunization with a recombinant cysteine-rich section of the Entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin A response that has in vitro adherence inhibition activity.
The LC3-encoded 52-kDa recombinant protein includes amino acids 758 to 1134 of the 170-kDa subunit of the galactose-inhibitable lectin. Oral immunization of BALB/c mice with the LC3-encoded protein and cholera holotoxin induced an intestinal secretory immunoglobulin A (IgA) response (P < 0.01 compared with the control). There was a negative correlation (P =
-
11. Oral Vaccination with Recombinant Yersinia enterocolitica Expressing Hybrid Type III Proteins Protects Gerbils from Amebic Liver Abscess
Protection against invasive amebiasis was achieved in the gerbil model for amebic liver abscess by oral immunization with live attenuated Yersinia enterocolitica expressing the Entamoeba histolytica galactose-inhibitable lectin that has been fused to the Yersinia outer protein E (YopE). Protection was dependent on the presence of the YopE translocation domai
American Society for Microbiology.
-
12. Carbohydrate binding and resistance to proteolysis control insecticidal activity of Griffonia simplicifolia lectin II
Griffonia simplicifolia leaf lectin II (GSII), a plant defense protein against certain insects, consists of an N-acetylglucosamine (GlcNAc)-binding large subunit with a small subunit having sequence homology to class III chitinases. Much of the insecticidal activity of GSII is attributable to the large lectin subunit, because bacterially expressed recombinan
The National Academy of Sciences.