Recombinant E Coli
Mostrando 1-12 de 1801 artigos, teses e dissertações.
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1. Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2)
Abstract Background: Crude venom of the banded tiger waspVespa affinis contains a variety of enzymes including hyaluronidases, commonly known as spreading factors. Methods: The cDNA cloning, sequence analysis and structural modelling of V. affinis venom hyaluronidase (VesA2) were herein described. Moreover, heterologous expression and mutagenesis of rVesA2
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 05/12/2019
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2. Identification, expression and characterization of the recombinant Sol g 4.1 protein from the venom of the tropical fire ant Solenopsis geminata
Abstract Background: Fire ant venom is a complex mixture consisting of basic piperidine alkaloids, various biologically active peptides and protein components, including a variety of major allergenic proteins. Tropical fire ant Solenopsis geminata is an important stinging ant species that causes anaphylaxis and serious medical problems. Although the biologi
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 21/09/2018
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3. Jaburetox: update on a urease-derived peptide
Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/02/2018
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4. Imunogenicidade da proteína M recombinante de Streptococcus equi subsp. equi coadministrada com um adjuvante molecular
ABSTRACT The strangles is an infectious disease that affects horses from all ages and causes important economic losses in the equine-related business. The aim of this work was to evaluate the immunogenicity of the recombinant M protein from Streptococcus equi (rSeM) co-administered with the recombinant heat-labile enterotoxin B subunit from Escherichia coli
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2017-10
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5. Molecular Characterization of a MYB Protein from Oryza sativa for its Role in Abiotic Stress Tolerance
ABSTRACT The MYB family represents one of the most abundant classes of transcriptional regulators that perform pivotal role under different developmental processes and abiotic stresses. In present study, a MYB gene from Oryza sativa was selected for functional characterization. Bioinformatics analysis revealed that OsMYB1 cDNA encodes R2-R3 type DNA binding
Braz. arch. biol. technol.. Publicado em: 22/06/2017
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6. Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis
Abstract Background The cysteine-rich neurotoxins from elapid venoms are primarily responsible for human and animal envenomation; however, their low concentration in the venom may hamper the production of efficient elapid antivenoms. Therefore, the aim of the present study was to produce fully active elapid neurotoxic immunogens for elapid antivenom produc
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 13/10/2016
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7. Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the
Braz. J. Microbiol.. Publicado em: 2016-09
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8. ON-LINE MONITORING OF BIOMASS CONCENTRATION BASED ON A CAPACITANCE SENSOR: ASSESSING THE METHODOLOGY FOR DIFFERENT BACTERIA AND YEAST HIGH CELL DENSITY FED-BATCH CULTURES
Abstract The performance of an in-situ capacitance sensor for on-line monitoring of biomass concentration was evaluated for some of the most important microorganisms in the biotechnology industry: Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Bacillus megaterium. A total of 33 batch and fed-batch cultures were carried out in a bench-scale b
Braz. J. Chem. Eng.. Publicado em: 2015-12
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9. Gene cloning, expression, and characterization of the Bacillus amyloliquefaciens PS35 lipase
Abstract Lipases are enzymes of immense industrial relevance, and, therefore, are being intensely investigated. In an attempt to characterize lipases at molecular level from novel sources, a lipase gene from Bacillus amyloliquefaciens PS35 was cloned, heterologously expressed in Escherichia coli DH5α cells and sequenced. It showed up to 98% homology with ot
Braz. J. Microbiol.. Publicado em: 09/10/2015
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10. Overexpression of Salmonella enterica serovar Typhi recA gene confers fluoroquinolone resistance in Escherichia coli DH5α
A spontaneous fluoroquinolone-resistant mutant (STM1) was isolated from its parent Salmonella enterica serovar Typhi (S. Typhi) clinical isolate. Unlike its parent isolate, this mutant has selective resistance to fluoroquinolones without any change in its sensitivity to various other antibiotics. DNA gyrase assays revealed that the fluoroquinolone resistance
Braz J Med Biol Res. Publicado em: 08/09/2015
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11. GPo1
alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortiumTo facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (
alkB ) gene ofPseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1alkB plasmid was transformed intoEscherichia coli DH5Braz. J. Microbiol.. Publicado em: 2015-09
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12. A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Abstract Background: The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/08/2015