Recombinant Allergens
Mostrando 1-12 de 19 artigos, teses e dissertações.
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1. Produção de alérgenos recombinantes relacionados a Der p 1 de Dermatophagoides pteronyssinus com potencial aplicação na avaliação da resposta imune humoral e celular em pacientes com alergias respiratórias
Dermatophagoides pteronyssinus (Dp) é uma importante espécie de ácaro da poeira domiciliar e está associado com doenças alérgicas. Alérgenos de Dp são causas importantes de sensibilização e resposta mediada por imunoglobulina E (IgE) entre pacientes com asma e rinite alérgica. O objetivo deste estudo foi produzir as proteínas recombinantes Der p
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/08/2011
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2. Produção de alérgeno Blo t 5 recombinante (rBlo t 5) de Blomia tropicalis e reatividade de anticorpos IgE a alérgenos Blo t 5 nativo e recombinante em pacientes atópicos
Allergies are caused by immunological reactions to allergens, which are substances capable of triggering specific IgE responses in genetically predisposed subjects. In tropical and subtropical regions, allergens derived from B. tropicalis are important causes of IgE-mediated sensitization among patients with asthma and allergic rhinitis. The aim of this stud
Publicado em: 2010
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3. Análise do transcriptoma da glândula produtora de veneno de Loxosceles intermedia (aranhamarrom): perfil de expressão e identificação de novas toxinas. / Effects of metalloproteinas from Brotrops leucurus venon and brown spiders venoms on endothelial cell and components of extracellular matrix.
Loxosceles genus spiders are responsible for accidents all over the world and have clinical importance in the South of Brazil. The venom of these spiders is made up of several toxins, including proteins, which are responsible for the clinical pattern called loxoscelism. To describe the transcriptional profile of the L. intermedia venom gland, we generated a
Publicado em: 2010
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4. Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli
Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR
Brazilian Journal of Medical and Biological Research. Publicado em: 2008-05
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5. Molecular Cloning and Characterization of Tropomyosin, a Major Allergen of Chironomus kiiensis, a Dominant Species of Nonbiting Midges in Korea
Chironomids are widely and abundantly distributed in the vicinity of standing waters. Larvae of Chironomus and some other genera are known to contain hemoglobins, which have been described as a major allergen, and the adults that have no hemoglobins also have been reported to contain allergens. In this study, we tried to establish the role of chironomid alle
American Society for Microbiology.
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6. Recombinant peanut allergen Ara h I expression and IgE binding in patients with peanut hypersensitivity.
Peanut allergy is a significant health problem because of the frequency, the potential severity, and the chronicity of the allergic sensitivity. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, w
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7. Surfactant proteins A and D protect mice against pulmonary hypersensitivity induced by Aspergillus fumigatus antigens and allergens
Allergic bronchopulmonary aspergillosis (ABPA) is an allergic disorder caused by an opportunistic fungal pathogen, Aspergillus fumigatus (Afu). Lung surfactant proteins SP-A and SP-D can interact with the glycosylated antigens and allergens of Afu, inhibit specific IgE binding to these allergens, and block histamine release from sensitized basophils. We have
American Society for Clinical Investigation.
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8. Role of C-Terminal Cysteine Residues of Aspergillus fumigatus Allergen Asp f 4 in Immunoglobulin E Binding
Among the several allergens cloned and expressed from Aspergillus fumigatus, Asp f 4 is a major one associated with allergic bronchopulmonary aspergillosis (ABPA). The structure-function relationship of allergens is important in understanding the immunopathogenesis, diagnosis, and treatment of allergic diseases. These include the epitopes, conformational or
American Society for Microbiology.
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9. Conversion of the major birch pollen allergen, Bet v 1, into two nonanaphylactic T cell epitope-containing fragments: candidates for a novel form of specific immunotherapy.
A novel approach to reduce the anaphylactic activity of allergens is suggested. The strategy makes use of the presence of conformational immunoglobulin E (IgE) epitopes on one of the most common allergens. The three dimensional structure of the major birch pollen allergen, Bet v 1, was disrupted by expressing two parts of the Bet v 1 cDNA representing amino
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10. Allergenic Characterization of Tropomyosin from the Dusky Brown Cockroach, Periplaneta fuliginosa
Household arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach, Periplaneta fuligino
American Society for Microbiology.
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11. The Anisakis simplex Ani s 7 major allergen as an indicator of true Anisakis infections
Ani s 7 is currently the most important excretory/secretory (ES) Anisakis simplex allergen, as it is the only one recognized by 100% of infected patients. The allergenicity of this molecule is due mainly to the presence of a novel CX17–25CX9–22CX8CX6 tandem repeat motif not seen in any previously reported protein. In this study we used this allergen as a
Blackwell Science Inc.
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12. The immunoglobulin-like modules Cε3 and α2 are the minimal units necessary for human IgE-FcεRI interaction
Atopic allergy is a genetically determined immunodisorder that affects almost 20% of the population worldwide. Immediate symptoms of type I allergy are caused by the release of biologic mediators from effector cells induced by IgE-allergen complexes that cross-link the high-affinity receptor for IgE (FcεRI). Chronic disease manifestations result from allerg
American Society for Clinical Investigation.