Real Time Pcr
Mostrando 25-36 de 1867 artigos, teses e dissertações.
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25. Toxoplasmosis in pregnancy: a clinical, diagnostic, and epidemiological study in a referral hospital in Rio de Janeiro, Brazil
ABSTRACT Toxoplasmosis in pregnant women can cause significant morbidity and mortality in the fetus, which may be mitigated by early diagnosis and treatment. Social factors have also been related to the risk of developing the congenital form of toxoplasmosis, since some of these factors interfere directly in the quality of prenatal care. This study aimed to
Braz J Infect Dis. Publicado em: 2020-12
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26. Blood microRNA expressions in patients with mild to moderate psoriasis and the relationship between microRNAs and psoriasis activity,
Abstract Background: In recent studies, microRNAs (mi-RNAs) have been shown to play an important role in psoriasis pathogenesis. However, studies evaluating mi-RNAs in the blood of psoriasis patients including a large number of mi-RNA panels are scarce. Objective: The authors aimed to assess mi-RNA expressions in blood samples of psoriasis patients, as wel
An. Bras. Dermatol.. Publicado em: 2020-12
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27. Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection
Abstract Background Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19). Although Real Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) of respiratory specimens is the gold standard test for detection of SARS-CoV-2 infection, collecting nasopharyngeal swabs causes discomfort to patie
Braz J Infect Dis. Publicado em: 2020-10
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28. Improvement of Mycobacterium tuberculosis detection in sputum using DNA extracted by sonication
Abstract Tuberculosis (TB) is one of the infectious diseases with high mortality in the world. DNA amplification techniques have been used to overcome barriers to the diagnosis of this disease. However, the success of these methodologies is highly dependent on the DNA obtained from the sample. This study was carried out to verify whether the DNA extracted by
Braz J Infect Dis. Publicado em: 2020-10
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29. Reverse-transcriptase polymerase chain reaction versus chest computed tomography for detecting early symptoms of COVID-19. A diagnostic accuracy systematic review and meta-analysis
ABSTRACT BACKGROUND: A positive real-time reverse-transcriptase polymerase chain reaction (RT-PCR) for SARS CoV-2, from nasopharyngeal swabs, is the current gold standard diagnostic test for this virus and has sensitivity of 60-70%. Some studies have demonstrated a significant number of false-negative RT-PCR tests while displaying significant tomographic f
Sao Paulo Med. J.. Publicado em: 2020-10
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30. An infant with a mild SARS-CoV-2 infection detected only by anal swabs: a case report
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China and has spread rapidly worldwide. We present a mild SARS-CoV-2 infection in a baby with non-productive cough and normal chest computed tomography, in whom only anal swabs tested positive by real-time PCR testing for SARS-CoV-2. She was given atomization inhalation t
Braz J Infect Dis. Publicado em: 2020-06
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31. Quantification of mitochondrial DNA damage and copy number in circulating blood of patients with systemic sclerosis by a qPCR-based assay,
Abstract Background: Although not fully understood, oxidative stress has been implicated in the pathogenesis of different autoimmune diseases such as systemic sclerosis. Accumulating evidence indicates that oxidative stress can induce mitochondrial DNA (mtDNA) damage and variations in mtDNA copy number (mtDNAcn). Objective: The aim of this study was to exp
An. Bras. Dermatol.. Publicado em: 2020-06
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32. Intrathyroid injection of dexamethasone inhibits Th2 cells in Graves’ disease
ABSTRACT Objective Intrathyroid injection of dexamethasone (IID) was used for decrease the relapse rate of hyperthyroidism in the treatment of Graves’ disease (GD), but the mechanism is still unclear. We aimed to explore the effect of IID on T help (Th)1/Th2 cells and their chemokine in patients with GD. Subjects and methods A total of 42 patients with
Arch. Endocrinol. Metab.. Publicado em: 2020-06
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33. Epidemiologia do esgoto como estratégia para monitoramento comunitário, mapeamento de focos emergentes e elaboração de sistemas de alerta rápido para COVID-19
The ability to monitor the spread of diseases is essential for prevention, intervention and control practices. In this context, wastewater-based epidemiology (WBE) has been applied to empirically evaluate the exposure of populations to chemical substances and pathogens of emerging concern in near real time. During the current COVID-19 pandemic, WBE remains a
Quím. Nova. Publicado em: 2020-04
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34. The rs11755527 polymorphism in the BACH2 gene and type 1 diabetes mellitus: case control study in a Brazilian population
ABSTRACT Objective Type 1 diabetes mellitus (T1DM) is an autoimmune disorder caused by a complex interaction between environmental and genetic risk factors. BTB domain and CNC homolog 2 (BACH2) gene encodes a transcription factor that acts on the differentiation and formation of B and T lymphocytes. BACH2 is also involved in the suppression of apoptosis an
Arch. Endocrinol. Metab.. Publicado em: 2020-04
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35. Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk
Abstract Staphylococcus aureus is the leading cause of intoxications in humans. Also, methicillin-resistant S. aureus (MRSA) is an emerging problem in food-producing animals. The presence of MRSA in milk may create a risk to public health. The aim of this study was to evaluate the presence of MRSA in bulk tank milk samples. One hundred and twenty bulk tank m
Food Sci. Technol. Publicado em: 2020-03
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36. Standardization of organoid culture for evaluation of melanogenesis induced by UVB, UVA and visible light,
Abstract Background: Organoid cultures are primary cultures that maintain architectural characteristics and the relationships between cells, as well as the extracellular matrix. They are alternatives for pathophysiological or therapeutic investigation rather than animal and in vitro tests. Objective: Development of a cutaneous organoid culture model, aimin
An. Bras. Dermatol.. Publicado em: 2020-02