Rapid Methods Microbiology
Mostrando 13-24 de 70 artigos, teses e dissertações.
-
13. Evaluation of a one-hour test for the identification of Neisseria species.
This study presents an evaluation of the RIM-N kit (Austin Biological Laboratories, Inc., Austin, Tex.), a commercial system for rapid identification of Neisseria spp. and Branhamella catarrhalis. The system was compared with the cystine-Trypticase (BBL Microbiology Systems, Cockeysville, Md.) agar method; 218 isolates were tested by each method. There was 9
-
14. Minitek urea disk test, a sensitive and cost-effective method to screen for Campylobacter pylori in gastric biopsies.
One hundred fifty-five biopsy specimens from the gastric mucosa of 81 patients undergoing routine endoscopy procedures were tested for the presence of Campylobacter pylori by three methods: Gram stain, culture, and modified Minitek, a rapid urea disk test (BBL Microbiology Systems, Cockeysville, Md.). Twenty-nine patients were infected with C. pylori. Sensit
-
15. Fluorogenic and chromogenic substrates used in bacterial diagnostics.
Methods based on the application of chromogenic and fluorogenic substrates enable specific and rapid detection of a variety of bacterial enzymatic activities. By using these techniques, enzymatic reactions can be examined simultaneously or individually, either directly on the isolation plate or in cell suspensions. For this purpose, various testing principle
-
16. Clinical laboratory evaluation of the automicrobic system Enterobacteriaceae biochemical card.
The AutoMicrobic System Enterobacteriaceae Biochemical Card (AMS-EBC; Vitek Systems, Inc.) was evaluated in two clinical microbiology laboratories. A total of 502 consecutive clinical isolates representing members of the family Enterobacteriaceae were tested in parallel with the AMS-EBC, API 20E, and Enterotube II systems. Discrepancies between systems were
-
17. Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study
Rapid and accurate identification of enterococci at the species level is an essential task in clinical microbiology since these organisms have emerged as one of the leading causes of nosocomial infections worldwide. Vibrational spectroscopic techniques (infrared [IR] and Raman) could provide potential alternatives to conventional typing methods, because they
American Society for Microbiology.
-
18. Phenotypic Identification of Actinomyces and Related Species Isolated from Human Sources
Recent advancements in chemotaxonomic and molecular biology-based identification methods have clarified the taxonomy of the genus Actinomyces and have led to the recognition of several new Actinomyces and related species. Actinomyces-like gram-positive rods have increasingly been isolated from various clinical specimens. Thus, an easily accessible scheme for
American Society for Microbiology.
-
19. Clinical laboratory applications of monoclonal antibodies.
Monoclonal antibody (MAb) technology is well recognized as a significant development for producing specific serologic reagents to a wide variety of antigens in unlimited amounts. These reagents have provided the means for developing a number of highly specific and reproducible immunological assays for rapid and accurate diagnosis of an extensive list of dise
-
20. Comparative study of seven commercial yeast identification systems.
AIMS: To compare the performance of seven commercial yeast identification methods with that of a reference method, and to compare the costs of the commercial kits. METHODS: Clinical yeast isolates (n = 52), comprising 19 species, were identified using Vitek, Api ID 32C, Api 20C AUX, Yeast Star, Auxacolor, RapID Yeast Plus system, and Api Candida and compared
-
21. Development of a Rapid PCR Assay Specific for Staphylococcus saprophyticus and Application to Direct Detection from Urine Samples
Staphylococcus saprophyticus is one of the most frequently encountered microorganisms associated with acute urinary tract infections (UTIs) in young, sexually active female outpatients. Conventional identification methods based on biochemical characteristics can efficiently identify S. saprophyticus, but the rapidities of these methods need to be improved. R
American Society for Microbiology.
-
22. Comparison of Commercial DNA Extraction Kits for Extraction of Bacterial Genomic DNA from Whole-Blood Samples
The demand for molecular diagnostic tests in medical microbiology has highlighted the need for efficient methods of DNA extraction. In addition, it is preferable for these methods to be automated. An example of such a requirement is for the confirmation of meningococcal disease where rapid, sensitive, and specific procedures are required for public health ma
American Society for Microbiology.
-
23. Comparison of the Staph-Ident System with a Conventional Method for Species Identification of Urine and Blood Isolates of Coagulase-Negative Staphylococci
The Staph-Ident system (Analytab Products) for species identification of coagulase-negative staphylococci was compared with the conventional method of Kloos and Schleifer (21). A total of 101 clinical isolates from urine cultures and 95 clinical isolates from blood cultures were studied: overall agreement between the two methods was 86%. We concluded that th
-
24. Automated direct antimicrobial susceptibility testing of microscopically screened urine cultures.
Two screening methods for urine microbiology are proposed: one in which the Gram-stained smear is used to detect significant bacteriuria, and another in which Autobac antibiotic susceptibility tests are performed directly on positive urine samples. Results on 1,350 specimens indicated that an average of 18 bacteria per oil immersion field were observed in th