Rapid Chemical Kinetic Technique
Mostrando 1-4 de 4 artigos, teses e dissertações.
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1. Inhibition mechanism of the nicotinic acetylcholine receptor α3β4 tacrine / Mecanismos de inibição do receptor nicotínico de acetilcolina α3β4 pela tacrina
Neuronal nicotinic acetylcholine (cholinergic) receptors (nAChRs) are integral membrane proteins and belong to the family of ligand-gated cation channels composed by α and β subunits. These receptors play a key role in the signal transmission between neurons in the central and peripheral nervous system. The α3β4 subtype, for example, is t
Publicado em: 2008
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2. Chemical kinetic measurements of a mammalian acetylcholine receptor by a fast-reaction technique.
In the presence of acetylcholine, the nicotinic acetylcholine receptor undergoes two rapid conformational changes: one in the 1-ms time region, leading to the formation of a transmembrane channel and signal transmission between cells, and the other in the 100-ms time region, leading to an inactive "desensitized" form with altered ligand-binding properties. T
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3. Dynamic properties of isolated acetylcholine receptor protein: kinetics of the binding of acetylcholine and Ca ions.
Kinetic and thermodynamic constants for elementary steps associated with the interaction of acetylcholine (AcCh) and Ca with isolated AcCh receptor from Torpedo californica have been determined by chemical relaxation spectrometry. Murexide as used as a Ca indicator to monitor changes in Ca bound to the AcCh receptor. In the presence of AcCh this technique pe
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4. Comparison of acetylcholine receptor-controlled cation flux in membrane vesicles from Torpedo californica and Electrophorus electricus: Chemical kinetic measurements in the millisecond region
In earlier studies with the acetylcholine receptor (AcChoR) of Electrophorus electricus the rate and equilibrium constants for a model that relates the ligand binding to ion translocation were determined, and the dependence of these constants on the concentrations of carbamoylcholine and acetylcholine, over a 200- and 5000-fold range, respectively, could be