Pythiosis
Mostrando 13-20 de 20 artigos, teses e dissertações.
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13. Immunodiffusion test for diagnosis and monitoring of human pythiosis insidiosi.
To facilitate the laboratory diagnosis of human cases of pythiosis insidiosi, an immunological test was evaluated. A soluble antigen was prepared from a human isolate of Pythium insidiosum, an aquatic, thermotolerant oomycete that causes infections in cattle, dogs, horses, and humans. Sera from seven proven cases of disseminated human pythiosis insidiosi wer
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14. Pythium insidiosum sp. nov., the etiologic agent of pythiosis.
Pythium insidiosum sp. nov., the etiologic agent of pythiosis, a cosmopolitan disease of horses, cattle, and dogs, is described and illustrated.
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15. Immunodiffusion test for diagnosing and monitoring pythiosis in horses.
A practical, sensitive, and specific immunodiffusion test was developed for diagnosing and monitoring pythiosis in horses. Culture filtrates, a soluble cell mass, and trypsinized Pythium sp. antigens were evaluated against prepared rabbit anti-Pythium sp. serum and pythiosis horse case sera. The culture filtrate antigens demonstrated the greatest capacity fo
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16. Immunoblot analysis of the humoral immune response to Pythium insidiosum in horses with pythiosis.
Reactions to Pythium insidiosum by sera from horses with active pythiosis were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Five strains of P. insidiosum were grown in nutrient broth and then sonicated. After centrifugation, supernatant antigens were separated by SDS-PAGE. An exoantigen of Conidiobo
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17. Development of a Species-Specific Probe for Pythium insidiosum and the Diagnosis of Pythiosis
Pythium insidiosum, the only species in the genus that infects mammals, is the etiological agent of pythiosis, a granulomatous disease characterized by cutaneous and subcutaneous lesions and vascular diseases. Accurate diagnosis of pythiosis and identification of its causal agent are often inconsistent with current immunological diagnostic methods. A species
American Society for Microbiology.
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18. Antigenic relationship between the animal and human pathogen Pythium insidiosum and nonpathogenic Pythium species.
Identification of the newly named pathogenic oomycete Pythium insidiosum and its differentiation from other Pythium species by morphologic criteria alone can be difficult and time-consuming. Antigenic analysis by fluorescent-antibody and immunodiffusion precipitin techniques demonstrated that the P. insidiosum isolates that cause pythiosis in dogs, horses, a
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19. Life cycle of the human and animal oomycete pathogen Pythium insidiosum.
Pythium insidiosum, the etiologic agent of pythiosis insidiosii, causes life-threatening infections in humans and animals. Previous studies of the epidemiology of this disease hypothesized about the possible life cycle of this oomycete. Details, however, were not provided on the steps required to cause infection. We investigated the life cycle of P. insidios
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20. Identification of Emerging Human-Pathogenic Pythium insidiosum by Serological and Molecular Assay-Based Methods
Pythium insidiosum is a pathogen that causes disease in both animals and humans. Human infection is rare; however, when it does occur, most patients, especially those having underlying hemoglobinopathy syndromes, such as thalassemia, exhibit a severe form. We identified four isolates of P. insidiosum. Two were recovered from tissue biopsy specimens from thal
American Society for Microbiology.