Pyrrolnitrin
Mostrando 1-12 de 23 artigos, teses e dissertações.
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1. Identificação e quantificação do gene pirrolnitrina (prnD) em Terra Preta Antropogênica da Amazônia por PCR em tempo real / Identification and quantification of pyrrolnitrin gene (prnD) in Anthropogenic Dark Earth by Real-time PCR
Anthropogenic Dark Earth (ADE) is considered one of the world\ s most fertile soils and receives this name because it originated from human action, probably by pre-Columbian populations who lived in these archaeological sites. Because of the common trend in agriculture towards sustainability antibiotic-producing bacteria is an alternative for biocontrol to p
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/08/2011
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2. Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin.
Pyrrolnitrin is a secondary metabolite of Pseudomonas and Burkholderia sp. strains with strong antifungal activity. Production of pyrrolnitrin has been correlated with the ability of some bacteria to control plant diseases caused by fungal pathogens, including the damping-off pathogen Rhizoctonia solani. Pseudomonas fluorescens BL915 has been reported to pro
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3. Metabolism of Tryptophans by Pseudomonas aureofaciens: VI. Production of Pyrrolnitrin by Selected Pseudomonas Species
Twenty-nine strains of Pseudomonas, classified as P. fluorescens biotype D or E or as P. multivorans, were examined for the production of pyrrolnitrin, an antifungal agent synthesized in P. aureofaciens. Eight strains were shown to produce pyrrolnitrin in shake-flask fermentation. Four cultures were from the multivorans taxon, and the remaining four were mem
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4. Mechanism of Action of the Antifungal Antibiotic Pyrrolnitrin
Pyrrolnitrin at 10 μg/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth inhibitory concentrations, pyrrolnitrin inh
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5. Cloning of Genes Involved in the Synthesis of Pyrrolnitrin from Pseudomonas fluorescens and Role of Pyrrolnitrin Synthesis in Biological Control of Plant Disease
A soil isolate of Pseudomonas fluorescens (BL915) was shown to be an effective antagonist of Rhizoctonia solani-induced damping-off of cotton. Investigation of the biological basis of this antagonism revealed that the strain produces pyrrolnitrin, a secondary metabolite known to inhibit R. solani and other fungi. Mutants of strain BL915 that did not produce
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6. Systemic Antifungal Activity of Pyrrolnitrin1
The antifungal activity of pyrrolnitrin, previously shown to be effective against superficial infections, was evaluated against experimental systemic mycoses. Pyrrolnitrin was inhibitory in vitro at <0.78 to 100 μg/ml to Candida albicans, Cryptococcus neoformans, Blastomyces dermatitidis, Sporotrichum schenckii, and Histoplasma capsulatum. Pyrrolnitrin acti
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7. Metabolism of Tryptophan by Pseudomonas aureofaciens: III. Production of Substituted Pyrrolnitrins from Tryptophan Analogues 1
Exogenous tryptophan is metabolized by Pseudomonas aureofaciens to yield pyrrolnitrin [3-chloro-4-(2′-nitro-3′-chlorophenyl)-pyrrole], an antifungal agent. The ability of this culture to metabolize tryptophan analogues in a similar manner was investigated by addition of the appropriate compound to the fermentation. Tryptophan precursors and metabolites o
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8. Effect of Pyrrolnitrin on Electron Transport and Oxidative Phosphorylation in Mitochondria Isolated from Neurospora crassa
Pyrrolnitrin, at low concentrations, uncouples oxidative phosphorylation in Neurospora mitochondria. At higher concentrations, pyrrolnitrin inhibits electron transport both in the flavine region and through cytochrome oxidase.
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9. Respiratory Chain of a Pathogenic Fungus, Microsporum gypseum: Effect of the Antifungal Agent Pyrrolnitrin
Pyrrolnitrin has been reported to inhibit Bacillus megaterium primarily by forming complexes with phospholipids and to block electron transfer of Saccharomyces cerevisiae between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. We found that pyrrolnitrin inhibited respiration of conidia of Microsporum gypseum. In mitochondrial pr
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10. Determination of Pyrrolnitrin and Derivatives by Gas-Liquid Chromatography
A gas-liquid chromatographic technique was applied to the separation of pyrrolnitrin and its derivatives. The simultaneous use of a flame detector and an electron capture detector made possible the distinction between the nitro derivatives of pyrrolnitrin and the other metabolites. The metabolites could be readily quantitated with the electron capture detect
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11. Functions Encoded by Pyrrolnitrin Biosynthetic Genes from Pseudomonas fluorescens
Pyrrolnitrin is a secondary metabolite derived from tryptophan and has strong antifungal activity. Recently we described four genes, prnABCD, from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. In the work presented here, we describe the function of each prn gene product. The four genes encode proteins identical in size and serology to
American Society for Microbiology.
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12. Pyrrolnitrin Production by Biological Control Agent Pseudomonas cepacia B37w in Culture and in Colonized Wounds of Potatoes
Bacterial strain B37w (= NRRL B-14858), an isolate noteworthy because it inhibits the growth of the bioherbicide fungus Colletotrichum truncatum, was selected for further studies of bacterial antifungal properties. This isolate was identified as a Pseudomonas cepacia strain by performing carbohydrate utilization and fatty acid profile analyses, as well as ot