Purification Protein
Mostrando 13-24 de 3311 artigos, teses e dissertações.
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13. Production, Purification and Physicochemical Properties of an Exo-Polygalacturonase from Aspergillus niger SW06
In this study, exo-polygalacturonase (exo-PG) production from Aspergillus niger SW06 was optimized by central composition design and high amount of 21.51 units mL-1 could be achieved in optimizing growth conditions. Both gel filtration and ion exchange chromatography revealed a single exo-PG activity peak, and sodium dodecyl sulfate polyacrylamide gel electr
J. Braz. Chem. Soc.. Publicado em: 2017-04
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14. Continuous blood purification treatment for endotoxin-induced acute respiratory distress syndrome
This study aimed to explore the effects of continuous blood purification (CBP) treatment in pigs affected with acute respiratory distress syndrome (ARDS). A total of 12 healthy male pigs, weighing 12±1.8 kg, were randomly and equally assigned to the control and experimental groups. The ARDS pig model was prepared by intravenous injections of endotoxin (20 �
Braz J Med Biol Res. Publicado em: 16/02/2017
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15. The physiological and biochemical mechanism of nitrate-nitrogen removal by water hyacinth from agriculture eutrophic wastewater
ABSTRACT Large amount of agriculturl wastewater containing high level nitrate-nitrogen (NO3 --N) is produced from modern intensive agricultural production management due to the excessive use of chemical fertilizers and livestock scale farming. The hydroponic experiment of water hyacinth was conducted for analyzing the content of NO3 --N, soluble sugar conten
Braz. arch. biol. technol.. Publicado em: 20/10/2016
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16. Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Abstract Background Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom ant
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 22/09/2016
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17. L-(+)-Lactic acid production by Lactobacillus rhamnosus B103 from dairy industry waste
ABSTRACT Lactic acid, which can be obtained through fermentation, is an interesting compound because it can be utilized in different fields, such as in the food, pharmaceutical and chemical industries as a bio-based molecule for bio-refinery. In addition, lactic acid has recently gained more interest due to the possibility of manufacturing poly(lactic acid),
Braz. J. Microbiol.. Publicado em: 2016-09
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18. Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a
Braz. J. Microbiol.. Publicado em: 2016-09
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19. A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri
Abstract Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (Xac), is one of the most devastating diseases to affect citrus crops. There is no treatment for citrus canker; effective control against the spread of Xac is usually achieved by the elimination of affected plants along with that of asymptomatic neighbors. An in dept
Braz. J. Microbiol.. Publicado em: 2016-06
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20. Sustainable Management of Keratin Waste Biomass: Applications and Future Perspectives
Keratin is a durable, fibrous protein which is mainly present in higher vertebrates (mammals, birds and reptiles) and humans epithelial cells. Food industry especially the meat market, slaughter house and wool industry produces million of tons of keratin containing biomass. These industries are constantly growing and the major producers include USA, Brazil a
Braz. arch. biol. technol.. Publicado em: 29/04/2016
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21. Cloning and purification of the first termicin-like peptide from the cockroach Eupolyphaga sinensis
Abstract Background Termicin is an antimicrobial peptide with six cysteines forming three disulfide bridges that was firstly isolated from the salivary glands and hemocytes of the termite Pseudacanthotermes spiniger. In contrast to many broad-spectrum antimicrobial peptides, termicin is most active against filamentous fungi. Although more than one hundred
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 19/02/2016
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22. Snake venom galactoside-binding lectins: a structural and functional overview
AbstractSnake venom galactoside-binding lectins (SVgalLs) comprise a class of toxins capable of recognizing and interacting with terminal galactoside residues of glycans. In the past 35 years, since the first report on the purification of thrombolectin from Bothrops atrox snake venom, several SVgalLs from Viperidae and Elapidae snake families have been descr
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 20/10/2015
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23. Extraction, purification and characterization of inhibitor of trypsin from Chenopodium quinoa seeds
Abstract A novel trypsin inhibitor of protease (CqTI) was purified from Chenopodium quinoa seeds. The optimal extracting solvent was 0.1M NaCl pH 6.8 (p < 0.05). The extraction time of 5h and 90 °C was optimum for the recovery of the trypsin inhibitor from C. quinoa seeds. The purification occurred in gel-filtration and reverse phase chromatography. CqTI pr
Food Sci. Technol. Publicado em: 03/10/2015
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24. pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
The functional characterisation of thousands of Trypanosoma cruzi genes remains a challenge. Reverse genetics approaches compatible with high-throughput cloning strategies can provide the tool needed to tackle this challenge. We previously published the pTcGW platform, composed by plasmid vectors carrying different options of N-terminal fusion tags based on
Mem. Inst. Oswaldo Cruz. Publicado em: 21/07/2015