Purification Of Proteins
Mostrando 13-24 de 1510 artigos, teses e dissertações.
-
13. Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 fromBothrops atrox snake venom
Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specim
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015
-
14. pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
The functional characterisation of thousands of Trypanosoma cruzi genes remains a challenge. Reverse genetics approaches compatible with high-throughput cloning strategies can provide the tool needed to tackle this challenge. We previously published the pTcGW platform, composed by plasmid vectors carrying different options of N-terminal fusion tags based on
Mem. Inst. Oswaldo Cruz. Publicado em: 21/07/2015
-
15. Construction of three new Gateway® expression plasmids for Trypanosoma cruzi
We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway® cassette were introduced into an e
Mem. Inst. Oswaldo Cruz. Publicado em: 21/11/2014
-
16. Cloning and Functional Assessment of the Recombinant Human Hepcidin-25 in the Baculovirus Expression System
Hepcidin is the primary regulatory hormone responsible for lowering the iron content in the blood circulation. Due to its biodegradability and low cytotoxicity, hepcidin is considered as an alternative for iron chelators. The baculovirus expression system may be suitable for human hepcidin production because the expressed proteins generally exhibit proper fo
Braz. arch. biol. technol.. Publicado em: 01/08/2014
-
17. Purification and characterization of a hyaluronidase from venom of the spider Vitalius dubius (Araneae, Theraphosidae)
BackgroundVenom hyaluronidase (Hyase) contributes to the diffusion of venom from the inoculation site. In this work, we purified and characterized Hyase from the venom of Vitalius dubius (Araneae, Theraphosidae), a large theraphosid found in southeastern Brazil. Venom obtained by electrical stimulation of adult male and female V. dubius was initially fractio
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 04/02/2014
-
18. Purification of metallo-B-lactamase CcrA from Bacteroides fragilis with salting-out method
A simple and effective salting-out method was developed for the purification of the metallo-β-lactamase CcrA from Bacteroides fragilis based on the plasmid pMSZ02, in which the crude protein secreted into growth medium was precipitated by 80% sulfate saturation of the medium and purified with Q-Sepharose to offer pure CcrA with yield of 20.1 mg per litter m
Braz. arch. biol. technol.. Publicado em: 2013-10
-
19. Antagonistic activity expressed by Shigella sonnei: identification of a putative new bacteriocin
Bacteriocins are antibacterial, proteinaceous substances that mediate microbial dynamics. Bacteriocin production is a highly disseminated property among all major lineages of bacteria, including Shigella. In this paper, we addressed the purification and characterisation of a bacteriocin produced by a Shigella sonnei strain (SS9) isolated from a child with ac
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-09
-
20. A simple procedure for the purification of active fractions in aqueous extracts of plants with allelopathic properties
Most studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purification of active compounds. In this study, we present a simple purification procedure that separates the active fraction of the extra
Acta Bot. Bras.. Publicado em: 2013-03
-
21. Novas estratégias de purificação dos fatores de coagulação Fator VIII e Proteína C a partir de plasma humano empregando cromatografia líquida. / New strategies of purification of coagulation Factor VIII and Protein C from human plasma using liquid chromatography.
Neste trabalho estudou-se a purificação de fator VIII de coagulação (FVIII) e da Proteína C (PC) por cromatografia. Em coluna ANX Sepharose FF como primeira etapa de purificação do plasma permite a eluição do FVIII e da PC com bom fator de purificação, mas não a separação destas. Propomos a separação de FVIII e PC empregando cromatografia de
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 19/03/2012
-
22. Distinct chitinases are expressed during various growth phases of the human pathogen Paracoccidioides brasiliensis
The aim of this work was the partial purification and subsequent evaluation of chitinase expression during the various growth phases of Paracoccidioides brasiliensis. Initially, PbCTS1r was expressed as a recombinant protein and displayed enzymatic activity against 4-MU-[N-acetylglucosamine (GlcNAc)]3 and 4-MU-(GlcNAc)2. Two proteins, 45 kDa and 39 kDa in si
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-05
-
23. An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up me
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 2012
-
24. Desenvolvimento de um adsorvente contínuo supermacroporoso de troca catiônica para recuperação de lactoferrina de soro de leite / Development of a cation exchange solid supermacroporous adsorbent for lactoferrin recovery from whey
Whey proteins have functional, nutritional and technological properties promoting their use as ingredients and supplements. Sweet whey obtained from Minas Frescal cheese production and concentrated by ultrafiltration was used in the lactoferrin capture in a supermacroporous adsorbent. Supermacroporous monolithic columns have been prepared by radical cryo-cop
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/11/2011