Protein Isolation And Purification
Mostrando 1-12 de 286 artigos, teses e dissertações.
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1. Application and comparison of proteolytic enzyme preparations in technology of protein hydrolyzates
Abstract Protepsin belongs to the class of acid proteinases, containing carboxyl groups in the active center. The preparation cleaves peptide bonds formed by aromatic amino acids (tyrosine, phenylalanine), and does not hydrolize esters and amides. Along with the proteolytic complex, Protepsin contains collagenase, lipase, and other digestive enzymes. The pre
Food Sci. Technol. Publicado em: 2020-06
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2. Application and comparison of proteolytic enzyme preparations in technology of protein hydrolyzates
Abstract Protepsin belongs to the class of acid proteinases, containing carboxyl groups in the active center. The preparation cleaves peptide bonds formed by aromatic amino acids (tyrosine, phenylalanine), and does not hydrolize esters and amides. Along with the proteolytic complex, Protepsin contains collagenase, lipase, and other digestive enzymes. The pre
Food Sci. Technol. Publicado em: 28/11/2019
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3. Isolation and purification of bromelain from waste peel of pineapple for therapeutic application
The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was
Braz. arch. biol. technol.. Publicado em: 2013-12
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4. The concept of two-dimensional electrophoresis-guided purification proven by isolation of rhodocetin from Calloselasma rhodostoma (Malayan pit viper)
Two-dimensional gel electrophoresis (2DE) is an important tool for investigating the complexity of snake venom proteomes. Apart from applications based on whole proteome analysis, we suggest that 2DE can be used as an assay to guide the progress of protein purification. The aim of this study was to prove the feasibility of this concept by using it to purify
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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5. Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold puri
Brazilian Archives of Biology and Technology. Publicado em: 2010-10
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6. Isolation and purification of complex II from proteus mirabilis strain ATCC 29245
A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa a
Brazilian Journal of Microbiology. Publicado em: 23/04/2010
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7. Hemolytic toxin from the soft coral Sarcophyton trocheliophorum: isolation and physiological characterization
The unifying characteristic of cnidarians is the production of protein and polypeptide toxins. The present study describes the identification of a hemolytic toxin from the soft coral Sarcophyton trocheliophorum. The crude extract was highly cytotoxic (EC50 = 50 ng/mL) against human erythrocytes. It was also tested for hemolytic activity by the blood agar pla
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 05/02/2010
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8. Purification and biochemica characterisation of endoplasmic reticulum α 1,2-mannosidase from Sporothrix schenckiil
Alpha 1,2-mannosidases from glycosyl hydrolase family 47 participate in N-glycan biosynthesis. In filamentous fungi and mammalian cells, α1,2-mannosidases are present in the endoplasmic reticulum (ER) and Golgi complex and are required to generate complex N-glycans. However, lower eukaryotes such Saccharomyces cerevisiae contain only one α1,2-mannosidase i
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-02
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9. Perfil protéico de sementes de acessos de cacaueiro no desenvolvimento do sabor de chocolate / Proteic profile from different accessions of cocoa seeds on the chocolate flavor development
Typical chocolate flavor is unique, only obtained from fermented, dried and roasted cocoa seeds, and can not be synthesized artificially. The flavor development is influenced by the genetic constitution, post-harvest processing and manufactures. Cotyledons proteins are believed to be the precursors of the chocolate flavor. The aim of the present work was to
Publicado em: 2010
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10. Development of a therapeutic vaccine against HPV16 / Desenvolvimento de vacina terapêutica contra HPV16
Cervical cancer is the second most common cancer among women worldwide. Most cases (83%) occur in developing countries, where they are found in relatively advanced stages and, consequently, the median survival is about 49% after five years. Therefore, an effective vaccine against HPV infections can lead to control of cancer of the cervix. Although preventabl
Publicado em: 2010
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11. Isolation, purification and pharmalogical and biochemical characterization of a new PLA2 (Bp12) from Bothrops pauloensis venom / Isolamento, purificação e caracterização bioquimica e farmacologica de uma nova fosfolipase A2 (Bp12) do veneno de Bothrops pauloensis
The myotoxic effects caused by bothropics venoms are unquestionable by their exuberant clinic manifestation and are extensively related in literature, since the neurotoxic effects have been described in vitro conditions, in nerve-muscle preparations, with little or neither clinic evidence. The aim of this study was to contribute for the knowledge about the p
Publicado em: 2008
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12. Avaliação do estresse oxidativo em ilhotas pancreáticas humanas e em cultura de células INS-1E / Evaluation of oxidative stress in human pancreatic islets and INS-1E cells culture
Human pancreatic islet transplantation is considered a promising strategy to cure the cure Diabetes Mellitus type I. However, transplantation efficiency is dramatically affected by sub-optimum islet recovery in the isolation/purification procedure and islet viability after transplantation. Isolated pancreatic islets are obtained through collagenase perfusion
Publicado em: 2007