Protein Immobilization
Mostrando 1-12 de 142 artigos, teses e dissertações.
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1. Imobilização enzimática: conceito e efeitos na proteólise
In recent years, great advances have been observed in the proteomic area regarding the different approaches adopted as top-down, middle-down and bottom-up. Regarding middle-down and bottom-up approaches, a previous step for protein digestion is necessary for further analysis of the generated peptides. Classical digestion protocols employing proteases are usu
Quím. Nova. Publicado em: 2020-04
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2. STABILIZATION STUDY OF TETRAMERIC Kluyveromyces lactis β-GALACTOSIDASE BY IMMOBILIZATION ON IMMOBEAD: THERMAL, PHYSICO-CHEMICAL, TEXTURAL AND CATALYTIC PROPERTIES
Abstract We investigated the immobilization of a tetrameric Kluyveromyces lactis β-galactosidase (EC: 3.2.1.23) (KL-Gal) on Immobead 150 using different support modification strategies. Immobead support was modified using an acid solution of H2SO4:HNO3 (3:1) (Immobead-Ac) or 5 % (v/v) glutaraldehyde (Immobead-Glu). Its unmodified form (Immobead) was also te
Braz. J. Chem. Eng.. Publicado em: 13/01/2020
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3. Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles
Abstract Polymer nanoparticles have been widely used in many biomedical applications, constituting a major incentive for immobilization of proteins. Poly(methyl methacrylate) nanoparticles were synthesized through miniemulsion polymerizations and used as supports for bovine serum albumin immobilization. Particularly, the effects of surfactant type (anionic s
Polímeros. Publicado em: 04/11/2019
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4. Treatment of sugarcane bagasse for the immobilization of soybean β-glucosidase and application in soymilk isoflavones
Resumo O bagaço de cana-de-açúcar, de resíduos agroindustriais, foi autoclavado a 121 °C durante 15 min, tratado com NaOH a 2% e ativado com 2,5% de glutaraldeído, para imobilização de β-glicosidase de soja. Análises de microscopia eletrônica de varredura, espectroscopia de raios X por dispersão de energia e espectroscopia de infravermelho por tr
Braz. J. Food Technol.. Publicado em: 17/10/2019
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5. Advances in enzyme bioelectrochemistry
ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein
An. Acad. Bras. Ciênc.. Publicado em: 2018
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6. Economy class syndrome: what is it and who are the individuals at risk?
Abstract The term 'economy class syndrome' refers to the occurrence of thrombotic events during long-haul flights that mainly occur in passengers in the economy class of the aircraft. This syndrome results from several factors related to the aircraft cabin (immobilization, hypobaric hypoxia and low humidity) and the passenger (body mass index, thrombophilia,
Rev. Bras. Hematol. Hemoter.. Publicado em: 2017-12
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7. Immobilization of myoglobin in sodium alginate composite membranes
Abstract The immobilization of myoglobin in sodium alginate films was investigated with the aim of evaluating the protein stability in an ionic polymeric matrix. Myoglobin was chosen due to the resemblance to each hemoglobin tetramer. Sodium alginate, being a natural polysaccharide, was selected as the polymeric matrix because of its chemica
Polímeros. Publicado em: 2015-06
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8. Surface characterization and osteoblast-like Cells culture on collagen modified PLDLA scaffolds
Surface modification techniques based on the grafting of chemical functional groups and immobilization of bioactive molecules have been used to improve biocompatibility and clinical performance of bioabsorbable scaffolds in tissue engineering and medicine regenerative applications. This study aimed at developing and characterizing a biomimetic surface to sti
Mat. Res.. Publicado em: 2014-12
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9. Myoglobin entrapment in poly(vinyl alcohol) dense membranes
Our goal in this study was the immobilization of myoglobin in poly(vinyl alcohol) dense membranes. Glutaraldehyde was investigated both as the crosslinking agent, aiming to increase the membrane stability in aqueous medium, and as the vehicle to bind myoglobin and PVA. Reaction and membrane synthesis were carried simultaneously in mild operating conditions i
Braz. J. Chem. Eng.. Publicado em: 2014-09
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10. Immobilization of puerarin glycosidase from Microbacterium oxydans CGMCC 1788 increases puerarin transformation efficiency
For immobilization of puerarin glycosidase from Microbacterium oxydans CGMCC 1788 on DEAE-52 cellulose, the optimal amount of enzyme protein was 12 mg protein: 1 g DEAE-52 cellulose; the optimal pH was 6.5; and the optimal immobilization time was 6 hr. The specific activity of immobilized enzyme was 36.67 mU.g-1 carrier with an immobilization yield of 98.87%
Braz. J. Chem. Eng.. Publicado em: 2014-06
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11. Effect of tibiotarsal joint inflammation on gene expression and cross-sectional area in rat soleus muscle
BACKGROUND: Joint inflammation is a common clinical problem in patients treated by physical therapists. The hypothesis of this study is that joint inflammation induces molecular and structural changes in the soleus muscle, which is composed mainly of slow-twitch muscle fibers. OBJECTIVE: To study the effect of tibiotarsal joint inflammation on muscle f
Braz. J. Phys. Ther.. Publicado em: 07/06/2013
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12. Biossegurança no controle da infecção hospitalar: desenvolvimento de imunossensor impedimétrico para detecção de Staphylococcus aureus em áreas críticas hospitalares / Biosafety in the control of hospital infection: development of an impedimetric immunosensor for detection of Staphylococcus aureus in critical areas of hospitals
The Biosafety is a very important tool for the Control of Hospital Infection establishing through methods, techniques and standards, measures to be adopted in order to prevent and control risks that may contribute to hospital infection. This work reports the development of an impedimetric immunosensor for detection of pathogenic bacteria, specifically Staphy
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 21/10/2011