Promoters Prokaryotic
Mostrando 1-12 de 82 artigos, teses e dissertações.
-
1. Rules extraction from neural networks applied to the prediction and recognition of prokaryotic promoters
Promoters are DNA sequences located upstream of the gene region and play a central role in gene expression. Computational techniques show good accuracy in gene prediction but are less successful in predicting promoters, primarily because of the high number of false positives that reflect characteristics of the promoter sequences. Many machine learning method
Genetics and Molecular Biology. Publicado em: 2011
-
2. Redes neurais artificiais aplicadas na caracterização e predição de regiões promotoras
A região promotora é uma seqüência de DNA que localiza-se anteriormente a uma determinada região gênica. Ela é responsável pelo início do processo de transcrição de um gene ou conjunto de genes. Assim, ela também atua como um elemento regulador da expressão gênica. O estudo da regulação da expressão gênica é relevante porque é essencial p
Publicado em: 2007
-
3. Recurrent neural nets for networks inference of gene interactions using Markov chains / Redes neurais recorrentes para inferência de redes de interação gênica utilizando cadeias de Markov
Array technologies have made it strainghtforward to simultaneously monitor the expression pattern of thousands of genes. Thus, a lot fot data is being generated and the challenge now is to discover how to extract useful information from these data sets. Microarray data is highly specialized. It involves several variables in a nonlinear and temporal way, dema
Publicado em: 2007
-
4. Reconhecimento e predição de promotores procarióticos: investigação de uma metodologia in silico baseada em HMMs
Gene expression on prokaryotes initiates when the RNA-polymerase enzyme interacts with DNA regions called promoters. In these regions are located the main regulatory elements of the transcription process. Despite the improvement of in vitro techniques for molecular biology analysis, characterizing and identifying a great number of promoters on a genome is a
Publicado em: 2005
-
5. Promoters responsive to DNA bending: a common theme in prokaryotic gene expression.
The early notion of DNA as a passive target for regulatory proteins has given way to the realization that higher-order DNA structures and DNA-protein complexes are at the basis of many molecular processes, including control of promoter activity. Protein binding may direct the bending of an otherwise linear DNA, exacerbate the angle of an intrinsic bend, or a
-
6. Ecologic Genomics of DNA: Upstream Bending in Prokaryotic Promoters
After our analysis of the distribution of predicted intrinsic curvature along all available complete prokaryotic genomes, the genomes were divided into two groups. Curvature distribution in all prokaryotes of the first group indicated a substantial fraction of promoters characterized by intrinsic DNA curvature located within or upstream of the promoter regio
Cold Spring Harbor Laboratory Press.
-
7. A prokaryotic tRNATyr gene, inactive in Xenopus laevis oocytes, is activated by recombination with an eukaryotic tRNAPro gene.
Eukaryotic tDNA promoters are composed of two essential regions contained within the coding sequence (Box A and Box B). Due to the highly conserved structure of prokaryotic and eukaryotic tRNA, most prokaryotic tRNA genes are expected to be active templates in eukaryotic transcriptional systems. In this paper we show that Escherichia coli tDNATyr is not tran
-
8. The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters
We constructed a library of synthetic promoters for Lactococcus lactis in which the known consensus sequences were kept constant while the sequences of the separating spacers were randomized. The library consists of 38 promoters which differ in strength from 0.3 up to more than 2,000 relative units, the latter among the strongest promoters known for this org
American Society for Microbiology.
-
9. In vitro transcription initiation of the spinach chloroplast 16S rRNA gene at two tandem promoters.
Two potential prokaryotic promoters, P1 and P2, are characterized 164 and 114 bp upstream of the spinach chloroplast 16S rRNA gene. The strengths of these promoters, calculated according to an homology score established for E. coli RNA-polymerase, are identical. Experiments performed with a Taq I-DNA fragment, containing 16 bp of the 16S rDNA and 243 bp upst
-
10. On the puzzling arrangement of the asymmetric MalT-binding sites in the MalT-dependent promoters.
The MalT-dependent promoters of the enterobacteria belong to a small family of positively regulated prokaryotic promoters in which the activator protein recognizes short asymmetric nucleotide sequences present in several locations and orientations. We demonstrate that active MalT-dependent semisynthetic promoters can be constructed by using a synthetic decan
-
11. Characterization of in vitro transcription initiation and termination sites in Col E1 DNA.
Overlapping restriction fragments from the region between the single Eco R1 site and the origin of replication of the plasmid, Col E1, have been utilised as templates in an in vitro transcription assay using E. coli RNA polymerase. Transcription towards the single Eco R1 site is initiated at a point 415 bp to the origin side of that site. In vivo, transcript
-
12. Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases
The plastid genome is known to be transcribed by a plastid-encoded prokaryotic-type RNA polymerase (PEP) and by a nucleus-encoded phage-type RNA polymerase (NEP). The spinach plastid rrn operon promoter region harbours three different, overlapping promoters. Two of them are of the prokaryotic type. The third promoter is a non-consensus-type NEP promoter. We
Oxford University Press.