Polyclonal Antibody
Mostrando 1-12 de 1277 artigos, teses e dissertações.
-
1. ANTICORPOS POLICLONAIS PARA O IMUNODIAGNÓSTICO DE VÍRUS BOVINO
Resumo O objetivo do estudo foi produzir anticorpos policlonais para vírus bovino e testar a reatividade em imunoensaios como imunofluorescência, imunoperoxidase e slot blot. Como imunógeno utilizou-se cepas e/ou isolados dos herpesvírus bovino tipo 1, 2 e 5 (BoHV-1, BoHV-2 e BoHV-5), vírus da diarreia viral bovina (BVDV), vírus respiratório sincicial
Ciênc. anim. bras.. Publicado em: 24/10/2019
-
2. Serum regucalcin is a useful indicator of liver injury severity in patients with hepatitis B virus-related liver diseases
Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal an
Braz J Med Biol Res. Publicado em: 30/09/2019
-
3. Influence of photobiomodulation therapy on root development of rat molars with open apex and pulp necrosis
Abstract This study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six grou
Braz. oral res.. Publicado em: 26/08/2019
-
4. Viruses infecting cool season crops in the northern Turkey
Abstract:Virus diseases of cool season vegetable crops (mainly cabbage, white and red head cabbage, broccoli, kale, radish, rocket salad, garden cress, and turnip) were surveyed in Bafra Plain, Turkey during winter 2017, and 2018. Leaf samples were collected from different species of the Brassicaceae family showing mosaic, mottling, necrotic spots, malformat
An. Acad. Bras. Ciênc.. Publicado em: 29/07/2019
-
5. Feline subcutaneous pythiosis
RESUMO: Pitiose em felinos é uma doença de ocorrência rara associada ao oomiceto Pythium insidiosum. O objetivo deste relato é descrever as características macroscópicas, histopatológicas e moleculares da infecção por P. insidiosum em um gato de dois anos de idade, com uma massa invasiva localizada no subcutâneo. O gato morreu logo após a cirurgi
Cienc. Rural. Publicado em: 11/03/2019
-
6. Advances and challenges in therapeutic monoclonal antibodies drug development
The use of serum containing polyclonal antibodies from animals immunized with toxins marked the beginning of the application of antibody-based therapy in late nineteenth century. Advances in basic research led to the development of the hybridoma technology in 1975. Eleven years later, the first therapeutic monoclonal antibody (mAb) was approved, and since th
Braz. J. Pharm. Sci.. Publicado em: 08/11/2018
-
7. Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples
Abstract In this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an IC50 value of 4.56 ng/mL towards DAN,
Food Sci. Technol. Publicado em: 30/07/2018
-
8. Development of an indirect ELISA assay to evaluation of the adaptive immune response of pacu (Piaractus mesopotamicus)
ABSTRACT The pacu is one of the most important species for Brazilian fish farming and is considered emerging in the global aquaculture. Despite its importance, no effective tool for evaluation of the adaptive immune response of this species has been developed. Therefore, this study aimed the development and standardization of indirect ELISA for the measureme
An. Acad. Bras. Ciênc.. Publicado em: 25/06/2018
-
9. Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene
Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The res
Braz. J. Microbiol.. Publicado em: 2018-06
-
10. Distribution of Ifitm3 in Yellow-Feathered Broilers and Inhibition of Avian Reovirus Multiplication by Ifitm3
ABSTRACT This study was carried to express the interferon-induced transmembrane protein 3 (IFITM3) in vitro and examine its function in inhibition of avian reovirus (ARV) replication. The recombinant prokaryotic vector expressing yellow-feathered broiler IFITM3 was successfully constructed, and the recombinant protein was expressed in competent Escherichia c
Braz. J. Poult. Sci.. Publicado em: 2018-04
-
11. Novel immunoassay for TSH measurement in rats
ABSTRACT Measuring thyroid hormones is an important aspect for the study of metabolism and for monitoring diseases in both human and animal models. The traditional method for hormone measurement in rats is the radioimmunoassay (RIA). However, the RIA is associated with some practical disadvantages, including the use of radioactive material, the need for spec
Arch. Endocrinol. Metab.. Publicado em: 18/09/2017
-
12. Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Abstract Background The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 06/07/2017