Pichia Pastoris
Mostrando 1-12 de 166 artigos, teses e dissertações.
-
1. Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid
Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical a
Food Sci. Technol. Publicado em: 2020-09
-
2. Expressão e caracterização da glicoproteína D do herpesvírus equídeo 1 em Pichia pastoris
RESUMO O herpesvírus equídeo 1 (EHV-1) apresenta distribuição mundial e causa graves prejuízos à equideocultura. É agente de surtos de doença respiratória, reprodutiva e neurológica, em equídeos jovens e adultos. A glicoproteína D (gD) do envelope viral é essencial para ligação e penetração em células permissivas e direcionamento do sistema
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2020-05
-
3. Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid
Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical a
Food Sci. Technol. Publicado em: 20/12/2019
-
4. Functional and biological insights of rCollinein-1, a recombinant serine protease from Crotalus durissus collilineatus
ABSTRACT Background: The prevalent class of snake venom serine proteases (SVSP) in Viperidae venoms is the thrombin-like enzymes, which, similarly to human thrombin, convert fibrinogen into insoluble fibrin monomers. However, thrombin-like serine proteases differ from thrombin by being unable to activate factor XIII, thus leading to the formation of loose c
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 08/04/2019
-
5. Optimization of an Activity Assay of Coprinus Cinereus Peroxidase
ABSTRACT To seek a simple, rapid and sensitive Coprinus cinereus Peroxidase (CIP) activity assay, a convenient one-factor-at-a-time (OFAT) method and a response surface methodology (RSM) were used. The recombinant CIP expressed in Pichia pastoris was purified with the Ni-NTA spin column. Based on the results of catalytic efficiency (kcat/Km) analysis, 2,2'-a
Braz. arch. biol. technol.. Publicado em: 14/11/2018
-
6. Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source
Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment a
Braz. J. Microbiol.. Publicado em: 2017-07
-
7. Production of recombinant human epidermal growth factor in Pichia pastoris
Abstract This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the medium can be affected by the fermentation condition
Braz. J. Microbiol.. Publicado em: 2017-06
-
8. ON-LINE MONITORING OF BIOMASS CONCENTRATION BASED ON A CAPACITANCE SENSOR: ASSESSING THE METHODOLOGY FOR DIFFERENT BACTERIA AND YEAST HIGH CELL DENSITY FED-BATCH CULTURES
Abstract The performance of an in-situ capacitance sensor for on-line monitoring of biomass concentration was evaluated for some of the most important microorganisms in the biotechnology industry: Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Bacillus megaterium. A total of 33 batch and fed-batch cultures were carried out in a bench-scale b
Braz. J. Chem. Eng.. Publicado em: 2015-12
-
9. Desempenho de larvas de jundiá, Rhamdia quelen, alimentadas com dietas contendo probióticos
Devido aos probióticos terem se mostrado uma alternativa viável ao uso de antibióticos como promotores de crescimento para animais, foi avaliado o desempenho, uniformidade e taxa de mortalidade de larvas de jundiá alimentadas com dietas contendo diferentes probióticos. Larvas de jundiá, com quatro dias pós-eclosão, foram alimentadas durante 21 dias c
Acta Sci., Anim. Sci.. Publicado em: 2015-09
-
10. Utilização de um ELISA baseado em NcSRS2 de Neospora caninum expressa em Pichia pastoris para diagnóstico de neosporose em ovinos e cães
A neosporose é uma doença causada pelo protozoário Neospora caninum que leva a perdas econômicas importantes em muitos países. No presente estudo, é descrita a utilização da proteína recombinante NcSRS2 de N. caninum expressa em Pichia pastoris em um ensaio imunoenzimático indireto (ELISA) para o diagnóstico de infecção por Neospora em ovelhas e
Rev. Bras. Parasitol. Vet.. Publicado em: 12/06/2015
-
11. Protease gene shuffling and expression in Pichia pastoris
Four kinds of neutral and alkaline protease genes from Aspergillus oryzae and Bacillus subtilis were isolated and shuffled. The shuffled genes were selected, inserted into pGAPZαA plasmid and transformed into Escherichia coli. The gene which could express high-activity protease was selected by screening the sizes of transparent zones around the colonies on
Braz. arch. biol. technol.. Publicado em: 2015-06
-
12. Recombinant hepatitis C virus-envelope protein 2 interactions with low-density lipoprotein/CD81 receptors
Hepatitis C virus (HCV) envelope protein 2 (E2) is involved in viral binding to host cells. The aim of this work was to produce recombinant E2B and E2Y HCV proteins in Escherichia coli and Pichia pastoris, respectively, and to study their interactions with low-density lipoprotein receptor (LDLr) and CD81 in human umbilical vein endothelial cells (HUVEC) and
Mem. Inst. Oswaldo Cruz. Publicado em: 26/05/2015