Phosphatases
Mostrando 13-24 de 788 artigos, teses e dissertações.
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13. Bioindicadores de qualidade de solo em diferentes ecossistemas
Changes due to soil management and cultivated plant species may influence carbon, nitrogen and phosphorus cycling. Cycling depends on the integration of physical, chemical and mainly microbiological processes. This work assessed some microbial activity and processes related to carbon, nitrogen and phosphorus cycles in soil from six ecosystems in Guarapuava,
Publicado em: 2009
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14. Production of D-myo-inositol(1,2,4,5,6)pentakisphosphate using alginate-entrapped recombinant Pantoea agglomerans glucose-1-phosphatase
The glucose-1-phosphatase encoding gene (agp) of Pantoea agglomerans was sequenced and heterologously expressed in Escherichia coli. The enzyme showed very high homology to periplasmatic glucose-1-phosphatases of other members of the Enterobacteriaceae family. It was isolated from transformed Escherichia coli cells in a single step in high yields (32.3 ± 1.
Brazilian Archives of Biology and Technology. Publicado em: 2008-04
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15. Atividade de fosfatases em duas cepas de Trypanosoma cruzi
Trypanosoma cruzi, the etiological agent of Chagas`disease, displays a remarkably high degree of both, structural and functional intraspecific heterogeneity, which could modulate its pathogenicity, survival and adaptability. In higher eukaryotes, phosphorylation and dephosphorylation are fundamental pathways that regulate a wide variety of cellular events. T
Publicado em: 2008
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16. Uma perspectiva computacional sobre catálise enzimática
Enzymes are extremely efficient catalysts. Here, part of the mechanisms proposed to explain this catalytic power will be compared to quantitative experimental results and computer simulations. Influence of the enzymatic environment over species along the reaction coordinate will be analysed. Concepts of transition state stabilisation and reactant destabilisa
Quím. Nova. Publicado em: 2008
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17. Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae)
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of stria
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2008
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18. Partial biochemistry characterization and obtention of low molecular weight acid phosphatase from osteoblasts cultures / Perfil da proteína tirosina fosfatase de baixo peso molecular em células osteoblásticas
Events as phosphorilation and desphosphorilation are experienced in cell growth and differentiation processes. Tyrosine phosphatases proteins are involved in these processes. These enzymes are found in animals, plants and occur in multiple forms, differing in relative molecular weight, substrate specificity and sensitivity to inhibitors. The enzymes that hav
Publicado em: 2008
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19. Natural compounds as modulators of transduction pathways in tumor cells / Copostos naturais como moduladores de vias de transdução de sinal em celulas tumorais
The search for compounds that specifically act on tumors, with low side effects, is a great aim in the studies related to cancer treatment. Many natural compounds isolated from plants have presented promising results as modulating agents of the signal transduction pathways in several types of tumors. Flavonoids, polyphenolic compounds largely distributed in
Publicado em: 2008
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20. BIOCHEMICAL AND PHYSIOLOGICAL EVALUATION OF POTATO CLONES IN RELATION TO ALUMINUM / AVALIAÇÃO BIOQUÍMICA-FISIOLÓGICA DE CLONES DE BATATA EM RELAÇÃO AO ALUMÍNIO
Aluminum (Al) is the most abundant metal in the earths crust, affecting thegrowth and development of plants. The objective of this work was to investigate and compare biochemical and physiological responses of potato clones, Macaca, SMIC148-A, Dakota Rose and Solanum microdontum, exposed to 0, 50, 100, 150 and 200 mg Al L-1 in nutrient solution (pH 4.0). Aft
Publicado em: 2008
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21. In vitro modulation of alkaline phosphatase activity of Saccharomyces cerevisiae grown in low or high phosphate medium
Our objective was to characterize the modulation of the activity of Saccharomyces cerevisiae alkaline phosphatases (ALPs) by classic inhibitors of ALP activity, cholesterol and steroid hormones, in order to identify catalytic similarities between yeast and mammalian ALPs. S. cerevisiae expresses two ALPs, coded for by the PHO8 and PHO13 genes. The product of
Brazilian Journal of Medical and Biological Research. Publicado em: 17/12/2007
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22. Influência da temperatura e de aditivos na matriz fosfolipídica usada para incorporação de fosfatases alcalinas pela técnica de Langmuir-Blodgett / Influence of the temperature and adtives in phospholipidic matrix utilized to Alkaline Phosphatases incorporation by Langmuir-Blodgett tecnique
ABSTRACT Alkaline phosphatases (AP) are unspecific enzymes that hydrolyze phosphate esthers. In this Dissertation we have studied the adsorption of two forms of AP in phospholipid Langmuir monolayers and Langmuir-Blodgett films, considered as biomembrane models, at different temperatures. Further, the effect of the presence of a disaccharide, trehalose, as w
Publicado em: 2007
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23. Definição de alvos moleculares em diferenciação, morte e resistencia de celulas tumorais / Defenition of molecular targets indifferentiation, death and resisteance in cancer cells
Cancer therapy efficiency, under several aspects, even with the progress of pharmacotechnology, remains as a challenge for the medicine. According to this factor, new agents that act on specific target, present low side-effects and prevent cancer cells escaping from death induction, are extremely desirable. In this work, 3 aspects of antitumoral prop
Publicado em: 2007
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24. Indicadores de qualidade do solo sob sistema de produção agrícola convencional em conversão para agroecológico
Microbial and biochemical indicators of soil quality, mainly those related to carbon and nitrogen cycling, are useful to access the effect of soil management and its effects on the sustainability of the agroecosystems. The aim of this work was to access changes occurring in the soil due to the transition from conventional to agroecological agricultural syste
Publicado em: 2007