Phage Typing
Mostrando 1-12 de 250 artigos, teses e dissertações.
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1. Phagetypes of Salmonella enteriditis isolated from clinical and food samples, and from broiler carcasses in southern Brazil
272 isolates of Salmonella Enteritidis (111 isolated from frozen broiler chicken carcasses, 126 from human food and other biological materials involved in food poisoning outbreaks and 35 from different poultry materials) were selected for phage typing. From these, 111 were phage typed, 57.65% being classified as phage type 4, 32.43% as phage type 4a, 3.60% a
Publicado em: 2010
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2. Phenotyping and genotyping methods applied to investigate the relatedness of brazilian isolates of enterobacter cloacae
In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic se
Publicado em: 2010
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3. Phenotypic and genotypic characterization of Salmonella enteritidis isolates
In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes a
Publicado em: 2010
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4. Salmonella spp. in raw broiler parts: occurrence, antimicrobial resistance profile and phage typing of the Salmonella enteritidis isolates
The present study was carried out to evaluate the occurrence of Salmonellae in raw broiler parts and to determine the antimicrobial resistance profile of the isolated strains. Twenty-four (39.3%) broiler parts samples were positive for Salmonella and twenty-five Salmonella strains were isolated, since two different serovars were detected in one single positi
Publicado em: 2010
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5. Tracing lineage by phenotypic and genotypic markers in Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and Salmonella Typhimurium isolated in state of São Paulo, Brazil
Fifty-three Salmonella 1,4,[5],12:i:- and 45 Salmonella Typhimurium strains were characterised using phage typing, plasmid profiles and pulsed-field gel electrophoresis (PFGE) for comparison. The majority of the strains were subdivided into definitive type (DT) 41 (22.6%) and DT 193 (18%) and the 60-MDa plasmid was detected in 94.3% and 84.4% of strains, res
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-11
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6. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by DNA-based typing methods 19 human and 25
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-08
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7. Salmonella spp : avaliação epidemiológica de surtos notificados no Paraná e caracterização de isolados epidêmicos e de origem avícola
Salmonella enterica subsp. enterica serovar Enteritidis (SE) is the main pathogen responsible for foodborne outbreaks in Paraná. Epidemiological surveillance of SE requires effective methods for subtyping and investigation of genetic diversity and origin of isolated ones, considering that this serovar presents high degree of genetic homogeneity. The use of
Publicado em: 2009
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8. Regional pattern of the molecular types of Cryptococcus neoformans and Cryptococcus gattii in Brazil
The molecular types of 443 Brazilian isolates of Cryptococcus neoformans and Cryptococcus gattii were analyzed to determine their geographic distribution within Brazil and their underlying host conditions. The following data, imported from previous epidemiological studies as well as two culture collections, were analyzed for: place of isolation, source (clin
Memórias do Instituto Oswaldo Cruz. Publicado em: 2008-08
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9. Antimicrobial susceptibility, phage types, and pulsetypes of Salmonella Typhimurium, in São Paulo, Brazil
A total of 283 Salmonella Typhimurium strains isolated from cases of human infections and non human sources, were examined for antimicrobial susceptibilityand the incidence of resistance was 38% and multiple resistance (to three or more antimicrobials) was 15%. All 43 multidrug-resistant strains (MDR) and 13 susceptible ones were characterized by phage typin
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-05
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10. SEROLOGICAL TYPING OF STAPHYLOCOCCUS AUREUS II. : Typing by Slide Agglutination and Comparison With Phage Typing
Cohen, Jay O. (Communicable Disease Center, Atlanta, Ga.), and P. B. Smith. Serological typing of Staphylococcus aureus. II. Typing by slide agglutination and comparison with phage typing. J. Bacteriol 88:1364–1371. 1964.—Over 700 cultures were typed by both antisera and phages; 15 serotypes accounted for 481 of the cultures. The i factor was found predo
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11. Improved procedure for bacteriophage typing of Listeria strains and evaluation of new phages.
A modified technique in listerial phage typing, termed reversed phage typing procedure, was developed. Ready-to-use typing plates were prepared by preapplication of phage suspensions on tryptose agar plates. The new procedure offers a number of substantial advantages as compared with the conventional method, being much more reliable, efficient, and convenien
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12. Epidemiologic Subtyping of Escherichia coli Serogroup O157 Strains Isolated in Ontario by Phage Typing and Pulsed-Field Gel Electrophoresis
Phage typing and DNA macrorestriction fragment analysis by pulsed-field gel electrophoresis (PFGE) were evaluated for use in the epidemiological subtyping of Escherichia coli serogroup O157 strains isolated in Ontario, Canada. Among 30 strains isolated from patients with sporadic cases of infection, 22 distinct XbaI macrorestriction patterns were identified
American Society for Microbiology.