Pga13
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Otimização do cultivo de Bacillus megaterium recombinante em bateladas alimentadas
Penicilinas acilases são enzimas de grande importância industrial, sendo utilizadas principalmente para produção de ácido 6 - aminopenicilânico, 6-APA, a partir de penicilina G. Em fins da década de 90, seu uso para síntese de antibióticos β- lactâmicos semi-sintéticos teve início em nível industrial, como uma alternativa no âmbito da quí
Publicado em: 2010
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2. Caracterização funcional dos genes PGA13 e PGA58 de Candida albicans / Functional characterization of PGA13 and PGA58 from Candida albicans
A incidência de infecções por fungos oportunistas na população de pacientes imunocomprometidos tem aumentado nos últimos anos, e estas são, principalmente, causadas por Candida albicans. Este patógeno oportunista pode crescer em diferentes formas, variando de levedura, pseudohifa e hifa e essa transição morfológica está associada com a virulênci
Publicado em: 2008
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3. Síntese enzimática de ampicilina com diferentes substratos em reator integrado
Penicilina G Acilase (PGA), EC 3.5.1.1.11, é uma enzima empregada nas reações de hidrólise da penicilina G para produção do ácido 6-amino penicilânico (6-APA), sendo também utilizada na síntese de antibióticos semi-sintéticos. Este trabalho levantou a influência de diferentes concepções de biocatalisador e de diferentes doadores acil sobre a s
Publicado em: 2008
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4. Regulation of phosphoglycerate phosphomutase in developing forespores and dormant and germinated spores of Bacillus megaterium by the level of free manganous ions.
The large depot of phosphoglyceric acid (PGA) which is accumulated within spores of Bacillus megaterium is greater than 99% 3-phosphoglyceric acid (3-PGA). The 3-PGA depot is stable in forespores and dormant spores, but is utilized rapidly during spore germination. When spores were germinated in KBr plus NaF, the PGA depot was not utilized, but 13% of the 3-
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5. DNA sequence analysis of pglA and mechanism of export of its polygalacturonase product from Pseudomonas solanacearum.
The pglA gene encodes a 52-kilodalton extracellular polygalacturonase (PGA) which is associated with the phytopathogenic virulence of Pseudomonas solanacearum. The nucleotide sequence of pglA and the putative amino acid sequence of the PGA protein were determined. A computer search identified a 150-residue region of PGA which was similar (41%) to the amino a
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6. T-DNA Insertional Mutagenesis for Activation Tagging in Rice1
We have developed a new T-DNA vector, pGA2715, which can be used for promoter trapping and activation tagging of rice (Oryza sativa) genes. The binary vector contains the promoterless β-glucuronidase (GUS) reporter gene next to the right border. In addition, the multimerized transcriptional enhancers from the cauliflower mosaic virus 35S promoter are locate
American Society of Plant Biologists.
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7. Preferential binding of growth inhibitory prostaglandins by the target protein of a carcinogen.
Liver fatty acid binding protein (L-FABP) is the principal target protein of the hepatic carcinogen N-(2-fluorenyl)acetamide (2-acetylaminofluorene) in rat liver. In addition, the cyclopentenone prostaglandins (PG), PGA, PGJ2, and delta 12-PGJ2, inhibit the growth of many cell types in vitro. This report describes the preferential binding of the growth inhib
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8. Cultivation of Bacteria Producing Polyamino Acids with Liquid Manure as Carbon and Nitrogen Source
Poly(γ-d-glutamic acid) (PGA)-producing strains of Bacillus species were investigated to determine their ability to contribute to reducing the amount of ammonium nitrogen in liquid manures and their ability to convert some of the ammonium into this polyamino acid as a transient depot for nitrogen. Organisms that do these things should help solve the serious
American Society for Microbiology.
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9. Toxin-Deficient Mutants from a Toxin-Sensitive Transformant of Cochliobolus Heterostrophus
Tox1 is the only genetic element identified which controls production of T-toxin, a linear polyketide involved in the virulence of Cochliobolus heterostrophus to its host plant, corn. Previous attempts to induce toxin-deficient (Tox(-)) mutants, using conventional mutagenesis and screening procedures, have been unsuccessful. As a strategy to enrich for Tox(-
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10. Proteomic Analysis of Candida albicans Cell Walls Reveals Covalently Bound Carbohydrate-Active Enzymes and Adhesins
Covalently linked cell wall proteins (CWPs) of the dimorphic fungus Candida albicans are implicated in virulence. We have carried out a comprehensive proteomic analysis of the covalently linked CWPs in exponential-phase yeast cells. Proteins were liberated from sodium dodecyl sulfate (SDS)-extracted cell walls and analyzed using immunological and advanced pr
American Society for Microbiology.
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11. Role of Murine Leukemia Virus Reverse Transcriptase Deoxyribonucleoside Triphosphate-Binding Site in Retroviral Replication and In Vivo Fidelity
Retroviral populations exhibit a high evolutionary potential, giving rise to extensive genetic variation. Error-prone DNA synthesis catalyzed by reverse transcriptase (RT) generates variation in retroviral populations. Structural features within RTs are likely to contribute to the high rate of errors that occur during reverse transcription. We sought to dete
American Society for Microbiology.
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12. A Gene Map of the Best’s Vitelliform Macular Dystrophy Region in Chromosome 11q12–q13.1
Best’s vitelliform macular dystrophy is an autosomal dominant disorder of unknown causes. To identify the underlying gene defect the disease locus has been mapped to an ∼1.4-Mb region on chromosome 11q12–q13.1. As a prerequisite for its positional cloning we have assembled a high coverage PAC contig of the candidate region. Here, we report the construc
Cold Spring Harbor Laboratory Press.