Peroxisomes
Mostrando 13-24 de 310 artigos, teses e dissertações.
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13. Efficient association of in vitro translation products with purified stable Candida tropicalis peroxisomes.
Newly synthesized peroxisomal proteins enter preexisting peroxisomes posttranslationally in vivo, generally without proteolytic processing. An efficient reconstitution of this process in vitro together with cloned DNAs for peroxisomal proteins would make possible investigation of the molecular information that targets proteins to peroxisomes. We have previou
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14. Firefly luciferase is targeted to peroxisomes in mammalian cells.
Although several enzymes known to reside in peroxisomes have been studied extensively, no cis-acting amino acid sequences involved in the transport of these proteins to peroxisomes have been described. As a first step towards the determination of a putative peroxisomal targeting sequence, we have expressed the cDNA encoding the firefly luciferase [Photinus-l
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15. Natural Senescence of Pea Leaves (An Activated Oxygen-Mediated Function for Peroxisomes).
We studied the activated oxygen metabolism of peroxisomes in naturally and dark-induced senescent leaves of pea (Pisum sativum L.). Peroxisomes were purified from three different types of senescent leaves and the activities of different peroxisomal and glyoxysomal enzymes were measured. The activities of the O2-- and H2O2-producing enzymes were enhanced by n
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16. Targeting of glyoxysomal proteins to peroxisomes in leaves and roots of a higher plant.
Higher plants possess several classes of peroxisomes that are present at distinct developmental stages and serve different metabolic roles. To investigate the cellular processes that regulate developmental transitions of peroxisomal function, we analyzed the targeting of glyoxysomal proteins to leaf-type and root peroxisomes. We transferred genes encoding th
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17. AtPex14p maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes
We previously isolated an Arabidopsis peroxisome-deficient ped2 mutant by its resistance to 2,4-dichlorophenoxybutyric acid. Here, we describe the isolation of a gene responsible for this deficiency, called the PED2 gene, by positional cloning and confirmed its identity by complementation analysis. The amino acid sequence of the predicted protein product is
Oxford University Press.
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18. Factors Affecting Development of Peroxisomes and Glycolate Metabolism among Algae of Different Evolutionary Lines of the Prasinophyceae.
Leaf-type peroxisomes are not present in the primitive unicellular Prasinophycean line of algae but are present in the multicellular algae Mougeotia, Chara, and Nitella, which are in the one evolutionary line, Charophyceae, that led to higher plants. Processes related to glycolate metabolism that may have been modified or induced with the appearance of perox
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19. Cytochemical Localization of Catalase Activity in Yeast Peroxisomes
Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles.
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20. Involvement of the Endoplasmic Reticulum in Peroxisome Formation
The traditional view holds that peroxisomes are autonomous organelles multiplying by growth and division. More recently, new observations have challenged this concept. Herein, we present evidence supporting the involvement of the endoplasmic reticulum (ER) in peroxisome formation by electron microscopy, immunocytochemistry and three-dimensional image re
The American Society for Cell Biology.
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21. Glycolate and Glyoxylate Metabolism by Isolated Peroxisomes or Chloroplasts 1
Chloroplasts, mitochondria, and peroxisomes from leaves were separated by isopycnic sucrose density gradient centrifugation. The peroxisomes converted glycolate-14C or glyoxylate-14C to glycine, and contained a glutamate: glyoxylate aminotransferase as indicated by an investigation of substrate specificity. The pH optimum for the aminotransferase was between
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22. Peroxisomes in wild-type and rosy mutant Drosophila melanogaster.
This study shows that peroxisomes are abundant in the Malpighian tubule and gut of wild-type Oregon R Drosophila melanogaster and that the peroxisomal population of the rosy-506 eye-color mutant differs from that of the wild type. Catalase activity in wild-type flies is demonstrable in bodies of appearance and centrifugal behavior comparable to the peroxisom
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23. Recognition of Peroxisomes by Immunofluorescence in Transformed and Untransformed Tobacco Cells.
We report the visualization of peroxisomes in tobacco (Nicotiana tabacum) leaves using fluorescently labeled antibodies to glycolate oxidase. In transgenic tobacco leaves the expression of isocitrate lyase was also visualized. In dual probing experiments both enzymes were shown to be present together in all peroxisomes in transgenic tobacco leaves.
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24. Association of glyoxylate and beta-oxidation enzymes with peroxisomes of Saccharomyces cerevisiae.
Although peroxisomes are difficult to identify in Saccharomyces cerevisiae under ordinary growth conditions, they proliferate when cells are cultured on oleic acid. We used this finding to study the protein composition of these organelles in detail. Peroxisomes from oleic acid-grown cells were purified on a discontinuous sucrose gradient; they migrated to th