Peptides Synthetase
Mostrando 1-12 de 42 artigos, teses e dissertações.
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1. Mapeamento do potencial biossintético em linhagens de Streptomyces / Mapping the biosynthetic potential in Streptomyces strains
Actinobacteria are important sources of new molecules with biological activities. Streptomyces are the most important genera studied. Such microorganisms carries out among a variety of biosynthetic enzimes, two main groups known as polyketide synthase (PKS) and non ribosomal peptide synthetase (NRPS), both catalyzing the biosynthesis of polyketide and non ri
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/07/2011
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2. A diversidade estrutural de peptídeos potenciadores da bradicinina da Bothrops jararaca (Bj-BPPs) proporciona ações sinérgicas no sistema cardiovascular. / The structural diversity of the proline-rich oligopeptides from Bothrops jararaca (Bj-BPPs) provides synergistic cardiovascular actions.
Nosso laboratório mostrou que um único gene codifica um precursor protêico, cujo processamento gera o peptídeo natriurético tipo C (CNP) e uma variedade de peptídeos ricos em prolina, conhecidos como peptideos potenciadores da bradicinina ou BPPs. Com pequenas diferenças, esse precursor é expresso na glândula do veneno e na região neuro-endócrina
Publicado em: 2010
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3. Implementation of Real Time Quantitative PCR technique (qPCR) for the monitoring of Microcystis and potentially microcystin-producing genotypes / Implementação da técnica de PCR Quantitativa em Tempo Real (qPCR) para o monitoramento de Microcystis e genótipos potencialmente produtores de microcistinas
Florações de cianobactérias tóxicas em corpos dágua doce usados como fonte para o consumo humano, recreação e irrigação são freqüentes nos dias de hoje devido à eutrofização destes ambientes. O monitoramento de linhagens tóxicas é importante para a prevenção dos efeitos adversos causados por suas toxinas na saúde de humanos e animais. Mét
Publicado em: 2008
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4. Estudo quimioenzimatico da sintese de um analogo do residuo N-terminal de nikkomicinas
Nikkomycins belong to a group of nucleoside peptides antibiotics produced by streptomycets. These compounds are potent chitin synthetase inhibitors and they exhibit fungicidal, insecticidal, and acaricidal activities. Our approach began with an asymmetric reduction of ethyl benzoylacetate 37. We evaluate the biocatalytic potential of some brazilian microorga
Publicado em: 2003
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5. Peptides at the tRNA binding site of the crystallizable monomeric form of E. coli methionyl-tRNA synthetase.
A protein affinity labeling derivative of E. coli tRNA(fMet) carrying lysine-reactive cross-linking groups has been covalently coupled to monomeric trypsin-modified E. coli methionyl-tRNA synthetase. The cross-linked tRNA-synthetase complex has been isolated by gel filtration, digested with trypsin, and the tRNA-bound peptides separated from the bulk of the
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6. Regulation of synthesis of hepatic fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes.
Mammalian fatty acid synthetase was shown to be composed of two peptides, molecular weight 240,000, after dissociation with sodium dodecyl sulfate. Rat liver polysomes that synthesize fatty acid synthetase were identified by sucrose gradient analysis of polysomes that had been reacted with 125I-labeled antibody against fatty acid synthetase. The binding of 1
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7. Genetic dissection of protein–protein interactions in multi-tRNA synthetase complex
Cytoplasmic aminoacyl-tRNA synthetases of higher eukaryotes acquired extra peptides in the course of their evolution. It has been thought that these appendices are related to the occurrence of the multiprotein complex consisting of at least eight different tRNA synthetase polypeptides. This complex is believed to be a signature feature of metazoans. In this
The National Academy of Sciences.
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8. Peptide Mapping of Aminoacyl-tRNA Synthetases: Evidence for Internal Sequence Homology in Escherichia coli Leucyl-tRNA Synthetase
Most aminoacyl-tRNA synthetases contain polypeptide chains of about either 50,000 or 100,000 daltons. Peptide mapping of tryptic, chymotryptic, or Staphylococcus aureus acid protease digests of seryl-tRNA synthetase (100,000, dimer) and leucyl-tRNA synthetase (100,000, monomer) from E. coli was done after selective modification of lysine residues with [14C]s
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9. Phylogenetic evidence for the early evolution of microcystin synthesis
Cyanobacteria are a prolific source of secondary metabolites, including compounds with toxic and enzyme-inhibiting activities. Microcystins and nodularins are the end products of a secondary metabolic pathway comprised of mixed polyketide synthases and nonribosomal peptide synthetases. Both peptides are potent natural toxins produced by distantly related gen
National Academy of Sciences.
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10. Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae.
We have screened a Saccharomyces cerevisiae expression library with antibodies against seryl-tRNA synthetase (SerRS) from baker's yeast. In this way we obtained clones which contain serS, the structural gene for seryl-tRNA synthetase. Genomic Southern blots show that the serS gene resides on a 5.0 kb SalI fragment. Nucleotide sequence analysis of the genes r
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11. Translation of rat liver fatty acid synthetase mRNA in a cell-free system derived from wheat germ
Total liver polysomes were isolated from rats that had fasted for 48 hr and that then had been re-fed a high-carbohydrate, fat-free diet for 20-24 hr. Indirect immunoprecipitation of the polysomes with purified antibody to rat liver fatty acid synthetase and deproteination on sodium dodecyl sulfate-containing sucrose gradients gave an RNA fraction which, whe
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12. The cloning and nucleotide sequence of cDNA for an amplified glutamine synthetase gene from the Chinese hamster.
The nucleotide sequence for a glutamine synthetase (GS) mRNA from gene-amplified Chinese hamster (CHO) cells was determined from recombinant cDNA clones obtained from both pBR322 and lambda gt10 libraries and by primer extension. The sequence obtained contains about 1400 bp corresponding to a minor species of mRNA terminated by a poly A sequence. The mRNA co