Peptide Conjugation
Mostrando 1-12 de 61 artigos, teses e dissertações.
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1. Radiolabeled nano-peptides show specificity for an animal model of human PC3 prostate cancer cells
OBJECTIVES: Cancer has been investigated using various pre-targeting techniques or models focusing on radiobombesin analogues; however, both are not offered together. In this study, nano-bombesin labeling by a pre-targeting system was undertaken to develop an alternative approach for prostate tumor treatment. METHODS: A two-step pre-targeting system utilizin
Clinics. Publicado em: 2011
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2. Development of immunoassay techniques to detect microcystin in environmental samples / Desenvolvimento de técnicas de imunoensaio para detecção de microcistina em amostras ambientais
The contamination of drinking water by cyanobacterial toxins is a public health issue and a concern for water authorities throughout the world. Microcystin-LR (MCLR) is a hazardous cyclic heptapeptide cyanotoxin, which inhibits protein phosphatase PP1 and PP2A in hepatocytes. Microcystins are produced by several genera of cyanobacteria and presents more than
Publicado em: 2009
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3. Development of a database for classification and analysis of type IV secretion systems / Desenvolvimento de um banco de dados para classificação e análise de sistemas de secreção do tipo IV bacteriano
The type IV secretion system can be classified as a large family of macromolecule transporters divided in three recognized sub-families involved in different bacterial functions. The major sub-family of T4SS is the conjugation system, which allows transfer of genetic material as a nucleoprotein via cell contact among bacteria. Analogously to bacterial conjug
Publicado em: 2008
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4. Mecanismos moleculares da neutralização da atividade letal das toxinas escorpiônicas por anticorpos monoclonais e policlonais
The major goal of this thesis is the immunochemical study of the scorpion toxins. Four manuscripts were presented concerning this subject. In the first report, a detoxified immunogen was prepared by conjugation of a toxic fraction (TstFG50), of the T. serrulatus venom, with bovine serum alburnin (BSA). The immunogen was inject in mice for inducing antibodies
Publicado em: 2007
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5. Immunologic modeling of a 75-kDa malarial protein with carrier-free synthetic peptides.
A protein of 75 kDa is produced in large quantities by the human malarial parasite Plasmodium falciparum and is present on the surface of the merozoite, whose function is to infect erythrocytes. Based on nucleotide sequence coding for 40% of this protein, two nonoverlapping model peptides 13 and 19 residues long were synthesized, coupled to a keyhole limpet
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6. Peptide conjugation to an in vitro-selected DNA ligand improves enzyme inhibition.
An in vitro selection technique was used to identify a specific high-affinity DNA ligand targeted to human neutrophil elastase (HNE). 1H NMR data and a comparative analysis of the selected sequences suggest that the DNA folds into a G-quartet structure with duplexed ends. The high-affinity binding DNA alone did not inhibit the enzymatic activity of HNE. The
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7. Hydrophobic peptide auxotrophy in Salmonella typhimurium.
The growth of a pleiotropic membrane mutant of Salmonella typhimurium with modified lipopolysaccharide composition was found to be strictly dependent on the peptone component of complex media. Nutritional Shiftdown into minimal media allowed growth for three to four generations. Of 20 commercial peptones, only enzymatic digests supported growth to varying de
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8. Covalent linkage of ribonuclease S-peptide to microinjected proteins causes their intracellular degradation to be enhanced during serum withdrawal.
The amino-terminal 20 amino acids are required for microinjected ribonuclease A (RNase A) to be taken up by lysosomes and degraded at an enhanced rate during serum withdrawal. We used water-soluble carbodiimides to covalently attach the RNase S-peptide (residues 1-20) to [3H]RNase S-protein (residues 21-124) at unspecified locations. We then measured catabol
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9. Folding and activity of hybrid sequence, disulfide-stabilized peptides.
Peptides have been synthesized that have hybrid sequences, partially derived from the bee venom peptide apamin and partially from the S peptide of ribonuclease A. The hybrid peptides were demonstrated by NMR spectroscopy to fold, forming the same disulfides and basic three-dimensional structure as native apamin, containing a beta-turn and an alpha-helix. The
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10. Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides
Triplex-forming oligonucleotides (TFOs) are DNA-binding molecules, which offer the potential to selectively modulate gene expression. However, the biological activity of TFOs as potential antigene compounds has been limited by cellular uptake. Here, we investigate the effect of cell-penetrating peptides on the biological activity of TFOs as measured in an as
Oxford University Press.
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11. Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation.
Conjugative transfer of the plasmid pCF10 by Enterococcus faecalis donor cells occurs in response to a peptide sex pheromone, cCF10, secreted by recipients. The plasmid-encoded cCF10 binding protein, PrgZ, is similar in sequence to binding proteins (OppAs) encoded by oligopeptide permease (opp) operons. Mutation of prgZ decreased the sensitivity of donor cel
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12. Identification of an Erythrocyte Binding Peptide from the Erythrocyte Binding Antigen, EBA-175, Which Blocks Parasite Multiplication and Induces Peptide-Blocking Antibodies
A biotinylated peptide covering a sequence of 21 amino acids (aa) from the erythrocyte binding antigen (EBA-175) of Plasmodium falciparum bound to human glycophorin A, an erythrocyte receptor for merozoites, as demonstrated by enzyme-linked immunosorbent assay (ELISA) and to erythrocytes as demonstrated by flow cytometry analysis. The peptide, EBA(aa1076–9
American Society for Microbiology.