Penicillin G Acylase
Mostrando 1-12 de 33 artigos, teses e dissertações.
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1. Solid-liquid equilibrium data of amoxicillin and hydroxyphenylglycine in aqueous media
The enzymatic synthesis of amoxicillin is catalyzed by Penicillin G Acylase (PGA). As byproducts, hydroxyphenylglycine and alcohol are also formed from hydrolytic reactions and antibiotic synthesis, respectively. The design of this process should be directed to promote the synthesis reaction. At the same time, it is necessary to reduce the hydrolytic reactio
Braz. J. Chem. Eng.. Publicado em: 2013-03
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2. Innovations in the enzymatic synthesis of amoxicillin / Inovações na síntese enzimática de amoxicilina
Penicilina G acilase (PGA, E.C.3.5.1.11) de Escherichia coli é uma enzima de grande importância industrial, amplamente utilizada para a hidrólise de penicilina G, produzindo o ácido 6-aminopenicilânico (6-APA), que é uma molécula chave para a síntese de penicilinas semi-sintéticas, dentre elas, a amoxicilina, que possui um amplo espectro de ação c
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/04/2012
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3. Improvement of penicillin G acylase expression in Escherichia coli through UV induced mutations
We used ultraviolet (UV) radiation to induce mutation in three locally isolated strains of Escherichia coli. Different dilutions of bacterial cultures were exposed to UV lamp of 254 nm wavelength for different time intervals at varied distances ranging from 5 to 210 sec and 5 to 100 cm. Viable colonies were screened for mutants with an increased production o
Brazilian Journal of Microbiology. Publicado em: 2010-12
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4. Otimização dinâmica do cultivo semi-contínuo de Pichia pastoris recombinante para produção das enzimas heterólogas alfa amilase e penicilina G acilase
Este mestrado se propoe a estudar a otimizacao dinamica de biorreator automatizado, tipo tanque agitado e aerado, em escala de bancada (ate 5L), onde se processa o cultivo semi-continuo de Pichia pastoris recombinante. Essa levedura foi clonada pelo grupo do prof. Fernando Torres, da UnB, utilizando o promotor PGK1, que dispensa a utilizacao de metanol como
Publicado em: 2010
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5. Inovações na produção de antibióticos β-lactâmicos
A produção industrial de ácido 6-aminopenicilânico (6-APA) inclui etapas de cultivo de Penicillium chrysogenum, extração com solvente orgânico, cristalização, hidrólise enzimática e precipitação. O interesse industrial e científico em reduzir o número de etapas deste processo tem motivado pesquisadores a buscar processos alternativos para obte
Publicado em: 2009
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6. Maintenance of penicillin G acylase expression by B. megaterium: preservation methods and activity recovery
This work reports the influence of different culture preservation methods on the production of penicillin G acylase (PGA) by Bacillus megaterium ATCC 14945. The initial stock culture, presenting PGA activity of 97 IU l-1, was preserved for two years using different procedures: monthly subculturing and storage in refrigerator (S), freeze-drying using skim mil
Brazilian Journal of Chemical Engineering. Publicado em: 2007-09
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7. Using a medium of free amino acids to produce penicillin g acylase in fed-batch cultivations of Bacillus megaterium ATCC 14945
The production of penicillin G acylase (PGA, an important industrial enzyme) from a wild strain of Bacillus megaterium using a pool of free amino acids as substrate was studied in a bench-scale bioreactor. Experiments carried out in shakers showed that the substitution of casein for free amino acids in the presence of cheese whey was the culture medium that
Brazilian Journal of Chemical Engineering. Publicado em: 2006-03
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8. Stabilization of penicillin G acylase by immobilization on glutaraldehyde-activated chitosan
The objective of this work was to study enzyme immobilization on chitosan activated with glutaraldehyde, aiming to produce a cheap biocatalyst. Two different immobilization strategies were studied: one-point and multipoint covalent attachment to the solid matrix. The multipoint covalent attachment derivative had an 82% immobilization yield. It was 4.9-fold m
Brazilian Journal of Chemical Engineering. Publicado em: 2005-12
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9. A fuzzy logic algorithm for identification of the harvesting threshold during PGA production by Bacillus megaterium
Penicillin G acylase (PGA) is an important enzyme used as biocatalyst in the production of semisynthetic beta-lactam antibiotics. Many microorganisms produce this enzyme and recombinant Escherichia coli has been preferred use for industrial applications. Bacillus megaterium is one of the microorganisms that excretes this enzyme into the medium. As a conseque
Brazilian Journal of Chemical Engineering. Publicado em: 2005-12
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10. Characterization of the penicillin G acylase from Bacillus megaterium ATCC 14945
O objetivo deste trabalho foi caracterizar a enzima penicilina G acilase (PGA) produzida por Bacillus megaterium, uma importante enzima industrial que catalisa a hidrólise de penicilina G, para produção de antibióticos semi-sintéticos. Purificação da enzima por ultra/diafiltração não permitiu detectar a banda de PGA por eletroforese SDS-PAGE devido
Brazilian Archives of Biology and Technology. Publicado em: 2005-06
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11. Improving selectivity and productivity of the enzymatic synthesis of ampicillin with immobilized penicillin G acylase
An experimental design was applied to improve the reaction conditions for enzymatic synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA), catalyzed by penicillin G acylase from E. coli immobilized on an agarose-glyoxyl derivative. The presence and magnitude of interactions between reaction variables were estimat
Brazilian Journal of Chemical Engineering. Publicado em: 2004-12
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12. Application of Fuzzy Logic in the Production of Penicillin G Acylase in Bacillus megaterium Cultivations. / Aplicação da lógica fuzzi na produção de penicilina G Acilase em cultivos de Bacillus megaterium.
Penicillin G acylase (PGA) is an important enzyme for the pharmaceutical industry. This enzyme has industrial use in the hydrolysis of penicillin G to obtain 6-aminocephalosporanic acid (6-APA), essential intermediate for the production of beta-lactam antibiotics. Many microorganisms produce PGA, but Bacillus megaterium is one of the few that excretes the en
Publicado em: 2003