Pectic Enzyme
Mostrando 13-24 de 46 artigos, teses e dissertações.
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13. Pectic Enzyme Activities of Bacteria Associated with Rotted Onions (Allium cepa)
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14. A Sycamore Cell Wall Polysaccharide and a Chemically Related Tomato Leaf Polysaccharide Possess Similar Proteinase Inhibitor-Inducing Activities 1
A large pectic polysaccharide, called rhamnogalacturonan I, that is solubilized by a fungal endo-α-1,4-polygalacturonase from the purified walls of suspension-cultured sycamore cells possesses proteinase inhibitor-inducing activity similar to that of the proteinase inhibitor-inducing factor, a pectic-like oligosaccharide fraction isolated from tomato leaves
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15. Changes in Cell Wall Polysaccharides of Green Bean Pods during Development
The changes in cell wall polysaccharides and selected cell wall-modifying enzymes were studied during the development of green bean (Phaseolus vulgaris L.) pods. An overall increase of cell wall material on a dry-weight basis was observed during pod development. Major changes were detected in the pectic polymers. Young, exponentially growing cell walls conta
American Society of Plant Physiologists.
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16. Activity Stain for Rapid Characterization of Pectic Enzymes in Isoelectric Focusing and Sodium Dodecyl Sulfate-Polyacrylamide Gels †
A system was developed for the rapid characterization of microbial pectic enzyme complexes and then tested on Erwinia chrysanthemi and Sclerotium rolfsii. Pectic enzymes in minute samples of crude culture filtrates were resolved by ultrathin-layer polyacrylamide gel isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then a
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17. Structure of Plant Cell Walls 1: XIX. Isolation and Characterization of Wall Polysaccharides from Suspension-Cultured Douglas Fir Cells
The partial purification and characterization of cell wall polysaccharides isolated from suspension-cultured Douglas fir (Pseudotsuga menziesii) cells are described. Extraction of isolated cell walls with 1.0 m LiCl solubilized pectic polysaccharides with glycosyl-linkage compositions similar to those of rhamnogalacturonans I and II, pectic polysaccharides i
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18. Comparison of pectic enzymes produced by Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, and Erwinia carotovora subsp. atroseptica.
Erwinia spp. that cause soft-rot diseases in plants produce a variety of extracellular pectic enzymes. To assess the correlation between patterns of pectic enzyme production and taxonomic classification, we compared the enzymes from representative strains. Supernatants obtained from polygalacturonate-grown cultures of nine strains of Erwinia chrysanthemi, th
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19. Purification and Properties of a Polygalacturonic Acid Trans-Eliminase Produced by Bacillus pumilus
A strain of Bacillus pumilus produced an extracellular pectic enzyme with polygalacturonic acid as the substrate. This enzyme, with optimal activity at pH 8.0 to 8.5, produced reaction products that strongly absorbed light at 232 nm, indicating the presence of a pectic acid trans-eliminase (PATE). Neither pectin esterase nor polygalacturonase was detected in
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20. Polygalacturonases Release Cell-Wall-bound Proteins 1
Purified polygalacturonases from two fungi released proteins from wall fractions prepared from three plant species. Peroxidase activity was associated with the proteins released from the cell walls, and several of the protein fractions released contained hydroxyproline. Cellulase, purified free of pectic enzyme activity, was ineffective in releasing cell wal
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21. The Catalytic Site of the Pectin Biosynthetic Enzyme α-1,4-Galacturonosyltransferase Is Located in the Lumen of the Golgi1
α-1,4-Galacturonosyltransferase (GalAT) is an enzyme required for the biosynthesis of the plant cell wall pectic polysaccharide homogalacturonan (HGA). GalAT activity in homogenates from pea (Pisum sativum L. var. Alaska) stem internodes co-localized in linear and discontinuous sucrose gradients with latent UDPase activity, an enzyme marker specific fo
American Society of Plant Physiologists.
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22. Elicitation of Casbene Synthetase Activity in Castor Bean 1: THE ROLE OF PECTIC FRAGMENTS OF THE PLANT CELL WALL IN ELICITATION BY A FUNGAL ENDOPOLYGALACTURONASE
Endopolygalacturonase isolated from culture filtrates of the fungus Rhizopus stolonifer was shown previously to act as an elicitor of biosynthetic capacity for the antifungal agent, casbene, in castor bean (Ricinus communis L.) seedlings (S.-C. Lee, C.A. West 1981 Plant Physiology 67:633-639). Selective amidation of exposed carboxyl groups of the pure fungal
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23. COMPARISON OF GROWTH AND PECTOLYTIC ENZYME PRODUCTION BY BACILLUS POLYMYXA
Nagel, Charles W. (University of California, Davis) and Reese H. Vaughn. Comparison of growth and pectolytic enzyme production by Bacillus polymyxa. J. Bacteriol. 83:1–5. 1962.—Studies were made of pectolytic enzyme production by Bacillus polymyxa during growth. It was found that elaboration of enzyme occurred during logarithmic growth and ceased when th
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24. Identification and Partial Characterization of the Pectin Methyltransferase “Homogalacturonan-Methyltransferase” from Membranes of Tobacco Cell Suspensions1
A membrane preparation from tobacco (Nicotiana tabacum L.) cells contains at least one enzyme that is capable of transferring the methyl group from S-adenosyl-methionine (SAM) to the C6 carboxyl of homogalacturonan present in the membranes. This enzyme is named homogalacturonan-methyltransferase (HGA-MT) to distinguish it from methyltransferases that c
American Society of Plant Physiologists.