P Pastoris
Mostrando 1-12 de 120 artigos, teses e dissertações.
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1. Expressão e caracterização da glicoproteína D do herpesvírus equídeo 1 em Pichia pastoris
RESUMO O herpesvírus equídeo 1 (EHV-1) apresenta distribuição mundial e causa graves prejuízos à equideocultura. É agente de surtos de doença respiratória, reprodutiva e neurológica, em equídeos jovens e adultos. A glicoproteína D (gD) do envelope viral é essencial para ligação e penetração em células permissivas e direcionamento do sistema
Arq. Bras. Med. Vet. Zootec.. Publicado em: 2020-05
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2. Optimization of an Activity Assay of Coprinus Cinereus Peroxidase
ABSTRACT To seek a simple, rapid and sensitive Coprinus cinereus Peroxidase (CIP) activity assay, a convenient one-factor-at-a-time (OFAT) method and a response surface methodology (RSM) were used. The recombinant CIP expressed in Pichia pastoris was purified with the Ni-NTA spin column. Based on the results of catalytic efficiency (kcat/Km) analysis, 2,2'-a
Braz. arch. biol. technol.. Publicado em: 14/11/2018
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3. Cultivation of Pichia pastoris carrying the scFv anti LDL (-) antibody fragment. Effect of preculture carbon source
Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment a
Braz. J. Microbiol.. Publicado em: 2017-07
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4. Production of recombinant human epidermal growth factor in Pichia pastoris
Abstract This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the medium can be affected by the fermentation condition
Braz. J. Microbiol.. Publicado em: 2017-06
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5. ON-LINE MONITORING OF BIOMASS CONCENTRATION BASED ON A CAPACITANCE SENSOR: ASSESSING THE METHODOLOGY FOR DIFFERENT BACTERIA AND YEAST HIGH CELL DENSITY FED-BATCH CULTURES
Abstract The performance of an in-situ capacitance sensor for on-line monitoring of biomass concentration was evaluated for some of the most important microorganisms in the biotechnology industry: Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Bacillus megaterium. A total of 33 batch and fed-batch cultures were carried out in a bench-scale b
Braz. J. Chem. Eng.. Publicado em: 2015-12
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6. Utilização de um ELISA baseado em NcSRS2 de Neospora caninum expressa em Pichia pastoris para diagnóstico de neosporose em ovinos e cães
A neosporose é uma doença causada pelo protozoário Neospora caninum que leva a perdas econômicas importantes em muitos países. No presente estudo, é descrita a utilização da proteína recombinante NcSRS2 de N. caninum expressa em Pichia pastoris em um ensaio imunoenzimático indireto (ELISA) para o diagnóstico de infecção por Neospora em ovelhas e
Rev. Bras. Parasitol. Vet.. Publicado em: 12/06/2015
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7. Protease gene shuffling and expression in Pichia pastoris
Four kinds of neutral and alkaline protease genes from Aspergillus oryzae and Bacillus subtilis were isolated and shuffled. The shuffled genes were selected, inserted into pGAPZαA plasmid and transformed into Escherichia coli. The gene which could express high-activity protease was selected by screening the sizes of transparent zones around the colonies on
Braz. arch. biol. technol.. Publicado em: 2015-06
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8. Effects of chemical treatments and environmental factors on seed dormancy and germination of shepherd's purse (Capsella bursa-pastoris (L.) Medic.)
Shepherd's purse (Capsella bursa-pastoris) is a problematic weed in citrus orchards and crop fields in northern Iran. In a series of laboratory and greenhouse experiments, we evaluated the effects that treatment with gibberellic acid (GA3) and potassium nitrate (KNO3), as well as environmental factors, including temperature, the duration of pre-chilling (wet
Acta Bot. Bras.. Publicado em: 2014-12
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9. Expressão heteróloga da EMA-2 (equi merozoite antigen) de Theileria equi em Pichia pastoris com potencial utilização em imunobiológicos
A piroplasmose equina causada por Theileria equi acomete os equinos de forma endêmica no Brasil e em diversos outros países tropicais e subtropicais. Considerada uma das mais importantes doenças de equinos, causa danos à saúde animal e perdas econômicas. A proteína equi merozoite antigen (EMA-2) é uma das principais proteínas de superfície, express
Cienc. Rural. Publicado em: 2014-10
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10. Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
Pichia pastoris is methylotrophic yeast used as an efficient expression system for heterologous protein production. In order to evaluate the effects of temperature (10 and 30 °C) and methanol (1 and 3% (v/v)) on genetically-modified Pichia pastoris, different biomarkers were evaluated: Heat stress (HSF-1 and Hsp70), oxidative stress (OGG1 and TBARS) and ant
Braz. J. Microbiol.. Publicado em: 2014-06
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11. HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
Pichia pastoris is a methylotrophic yeast used as an efficient expression system for heterologous protein production as compared to other expression systems. Considering that every cell must respond to environmental changes to survive and differentiate, determination of endogenous protein related to heat stress responses and hypoxia, it would necessary to es
Braz. J. Microbiol.. Publicado em: 2014-06
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12. Cloning, characterization and expression of a novel laccase gene Pclac2 from Phytophthora capsici
Laccases are blue copper oxidases (E.C. 1.10.3.2) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O2 to H2O. A novel laccase gene pclac2 and its corresponding full-length cDNA were cloned and characterized from Phytophthora capsici for the first time. The 1683 bp ful
Braz. J. Microbiol.. Publicado em: 08/04/2014