Ovomucoid
Mostrando 1-12 de 25 artigos, teses e dissertações.
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1. Analysis of binding properties and specificity through identification of the interface forming residues (IFR) for serine proteases in silico docked to different inhibitors.
Background: Enzymes belonging to the same super family of proteins in general operate on variety of substrates and are inhibited by wide selection of inhibitors. In this work our main objective was to expand the scope of studies that consider only the catalytic and binding pocket amino acids while analyzing enzyme specificity and instead, include a wider cat
BMC Structural Biology. Publicado em: 2011
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2. Study of partition of ovomucoid protein in aqueous twophase systems and anionic or hydrophobic resins / Estudo da partição da proteína ovomucoide em sistemas aquosos bifásicos e em resinas aniônica ou hidrofóbica
In this work, the application of the liquid-liquid extraction technique with aqueous two-phase systems (ATPS) was evaluated for the separation of ovomucoid, an egg white protein. This technique is an advisable purification process applied to large scale since it provides a selective partition with high yields. The ATPS were composed by PEG 1500 and an inorga
Publicado em: 2009
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3. Efeito da radiação gama em proteína alergênica de ovos de galinhas poedeiras / Gamma radiation effect on allergen protein of laying hen eggs
O ovo é o alimento naturalmente mais completo, uma vez que possui todos os nutrientes necessários, como vitaminas, aminoácidos e minerais essenciais para manter uma vida. Porém, em contra partida, possui várias proteínas promotoras de alergias em considerável parcela da população mundial. Para determinar as proteínas dos alimentos alergênicos, um
Publicado em: 2009
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4. Isolation and characterization of the chicken ovomucoid gene.
The chicken ovomucoid gene has been isolated by screening a chicken DNA library with a plasmid containing ovomucoid mRNA sequences. Twelve recombinant phages carrying ovomucoid mRNA sequences were isolated. Two of them, extending farthest into the 5' and 3' direction respectively, were characterized by restriction mapping and Southern hybridization as well a
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5. Heterogeneous initiation regions for transcription of the chicken ovomucoid gene.
We have identified the 5' termini of ovomucoid RNA transcripts isolated from estrogen stimulated chick oviduct by S1-nuclease mapping. Most of the ovomucoid RNA molecules (88-89%) are initiated at a predominant region which is positioned at about 30 basepairs downstream from a "TATATAT" sequence. However, there is at least one additional initiation region wh
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6. Avian P1 antigens inhibit agglutination mediated by P fimbriae of uropathogenic Escherichia coli.
Whole egg white from pigeon, dove, and cockatiel eggs, as well as the ovomucoid fraction of pigeon egg white, exhibited strong P1 antigenic activities and inhibited agglutination of human P1 erythrocytes and of digalactoside-coated latex beads by P-fimbriated Escherichia coli strains. In contrast, chicken egg white exhibited only weak P1 antigenic activity a
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7. Refined crystal structure of the molecular complex of Streptomyces griseus protease B, a serine protease, with the third domain of the ovomucoid inhibitor from turkey.
We have determined the crystal structure of the molecular complex between Streptomyces griseus protease B (SGPB), a bacterial serine protease, and the third domain of the ovomucoid inhibitor from turkey. Restrained-parameter least-squares refinement of the structure with the 1.8-A intensity data set has resulted in an R factor of 0.125. The carbonyl carbon a
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8. The galactosyl-(alpha 1-4)-galactose-binding adhesin of Streptococcus suis: occurrence in strains of different hemagglutination activities and induction of opsonic antibodies.
The occurrence of the galactose-(alpha 1-4)-galactose-specific adhesin in Streptococcus suis, a pig and human pathogen causing sepsis, meningitis, and other serious infections, was studied. Poly- and monoclonal anti-bodies to the purified adhesin, as well as pigeon ovomucoid, a specific probe for the adhesin activity, detected one single protein band in extr
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9. Effect of Polycations, Polyanions, and Neuraminidase on the Infectivity of Trachoma-Inclusion Conjunctivitis and Lymphogranuloma Venereum Organisms in HeLa Cells: Sialic Acid Residues as Possible Receptors for Trachoma-Inclusion Conjunctivitis
The infectivity of trachoma-inclusion conjunctivitis (TRIC) organisms (TW-5) was enhanced by pretreatment of HeLa cell monolayers before inoculation with diethylaminoethyl (DEAE)-dextran (30 μg/ml) and poly-l-lysine (10 μg/ml) and inhibited by dextran sulphate (250 μg/ml), fetuin (4%), ovomucoid (5%), N-acetyl neuraminic acid (0.5%), and Cholera vibrio ne
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10. Osteonectin cDNA sequence reveals potential binding regions for calcium and hydroxyapatite and shows homologies with both a basement membrane protein (SPARC) and a serine proteinase inhibitor (ovomucoid).
Osteonectin is a prominent noncollagenous protein of developing bone. A 2150-base-pair cDNA coding for osteonectin, isolated from a bovine bone cell lambda gt11 expression library, was sequenced and identified by comparison with protein sequence data. The nucleotide sequence predicts that osteonectin contains 304 amino acids, including a 17-residue signal pe
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11. Factors Affecting the Growth of Pseudomonas fluorescens in Liquid Egg White
Pseudomonas fluorescens grew rapidly in fresh egg albumen diluted with water. Growth of the bacteria in egg albumen was stimulated by the addition of carbohydrate and ovomucoid-rich egg exudate. Polyacrylamide-gel electrophoresis for residual egg albumen revealed extensive proteolysis of albumen inoculated with the organism. A fluorescent compound with absor
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12. Modulation of protein–protein interactions by synthetic receptors: Design of molecules that disrupt serine protease–proteinaceous inhibitor interaction
In the present article we describe the design and evaluation of a synthetic receptor that binds to the exterior surface of chymotrypsin and disrupts its interaction with proteinaceous inhibitors, such as soybean trypsin inhibitor, basic pancreatic trypsin inhibitor, ovomucoid turkey inhibitor, and Bowman-Birk inhibitor. Using enzyme kinetics, nondenaturing g
National Academy of Sciences.