Organotypic Cultures
Mostrando 1-12 de 84 artigos, teses e dissertações.
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1. Investigação do efeito neuroprotetor da atorvastatina em um modelo in vitro de toxicidade induzida pelo peptídeo ¿-amiloide
Alzheimer¿s disease is an age-related neurodegenerative disorder characterized by progressive memory loss, inability to perform the activities of daily living and personality changes. AD is characterized histopathologically by the presence of senile plaques, neurofibrillary tangles, synapse loss and neuronal death. Moreover, it is now recognized that neuroi
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2012
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2. Avaliação do potencial neuroprotetor do 17β-estradiol em fatias de cultura organotípica de hipocampo de rato submetidas à privação de oxigênio e glicose / Evaluatuion of the potential neuroprotection of 17-estradiol in organotypic slice cultures forat hippocampus exposed to oxygen and glucose deprivation
O cérebro é dependente de um fluxo sanguíneo contínuo para suplemento de glicose e oxigênio. A isquemia cerebral resulta na degeneração celular e perda da sua funcionalidade. Culturas organotípicas representam um modelo in vitro que permite estudar o dano cerebral isquêmico. Neste trabalho nós investigamos o efeito neuroprotetor do 17b-estradiol nu
Publicado em: 2010
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3. Resveratrol-loaded nanocapsules treatment reduces in vitro and in vivo glioma growth
Glioblastoma multiforme is a devastating cerebral tumor that submits to an awful prognostic. Because of this, improvements in the treatments are necessary to decrease the side effects and to increase the survival of the patients. Resveratrol, a constituent of the red wine, has been described to inhibit carcinogenesis at multiple stages. Biodegradable nanopar
Publicado em: 2010
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4. Secreção de hormônio de crescimento de camundongo por queratinícitos humanos primários: perspectivas para um modelo animal de terapia gênica cutânea / SECRETION OF MOUSE GROWTH HORMONE BY TRANSDUCED PRIMARY HUMAN KERATINOCYTES: PROSPECTS FOR AN ANIMAL MODEL OF CUTANEOUS GENE THERAPY
Keratinocytes are a very attractive vehicle for ex vivo gene transfer and systemic delivery, since proteins secreted by these cells may reach the circulation via a mechanism which mimics the natural process. An efficient retroviral vector (LXSN) encoding the mouse growth hormone gene (mGH) was used to transduce primary human keratinocytes. Organotypic raft c
Publicado em: 2008
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5. Efeito da rapamicina em culturas organotípicas de queratinócitos que expressam oncoproteínas de papiloma vírus humano tipo 16 / Effect of rapamycin in organotypic cultures of keratinocytes expressing oncoproteins of Papillomavirus type 16
A infecção por HPV de alto risco é considerada um dos principais fatores de risco para o desenvolvimento do carcinoma do colo uterino, um das neoplasias mais freqüentes em mulheres de todo o mundo. As oncoproteínas E6 e E7 de HPV-16 são capazes de induzir a degradação dos genes supressores de tumor p53 e pRb, respectivamente. Mais do que isso, a expr
Publicado em: 2007
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6. An in vitro blood–brain barrier model: Cocultures between endothelial cells and organotypic brain slice cultures
This communication describes a novel in vitro blood–brain barrier (BBB) model: organotypic slice cultures from the central nervous system were overlaid on endothelial cell monolayers grown on permeable membranes. Morphological, electrophysiological, and microdialysis approaches were carried out to characterize and validate this model. After 10 days in cocu
The National Academy of Sciences.
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7. Two Novel Promoters in the Upstream Regulatory Region of Human Papillomavirus Type 31b Are Negatively Regulated by Epithelial Differentiation
Organotypic cultures support the stratification and differentiation of keratinocytes and the human papillomavirus (HPV) life cycle. We report transcription from four novel promoters in the HPV31b upstream regulatory region during the viral life cycle in organotypic cultures. Promoter initiation was not differentiation dependent; two promoters were down-regul
American Society for Microbiology.
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8. Antivaccinia Activities of Acyclic Nucleoside Phosphonate Derivatives in Epithelial Cells and Organotypic Cultures
Organotypic “raft” cultures of epithelial cells allow the reconstitution of a skin equivalent that is easily infectible with different viruses with cutaneous tropism. Among these, poxvirus and particularly vaccinia virus (VV) are good candidates for use in antiviral tests, giving histological pictures comparable to those observed in humans infected with
American Society for Microbiology.
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9. Application of Real-Time Fluorescent PCR for Quantitative Assessment of Neospora caninum Infections in Organotypic Slice Cultures of Rat Central Nervous System Tissue
The previously described Nc5-specific PCR test for the diagnosis of Neospora caninum infections was used to develop a quantitative PCR assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. The quantitative PCR was performed by using a dual fluorescent hybridization probe system and the Light
American Society for Microbiology.
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10. Patch-clamp recording of amino acid-activated responses in "organotypic" slice cultures.
Patch-clamp recording techniques were used to study the properties of amino acid-activated channels in cultured "organotypic" slices from rat cerebellum and hippocampus. Hippocampal pyramidal cells responded to the three main glutamatergic agonists, N-methyl-D-aspartate (N-Me-D-Asp), quisqualate, and kainate, whereas Purkinje cells responded only to quisqual
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11. Cell-associated subacute sclerosing panencephalitis agent studied in organotypic central nervous system cultures: viral rescue attempts and morphology.
Organotypic cultures of hamster cerebellum were exposed to the IP-3-Ca cell line , which contains a cell-associated subacute sclerosing panencephalitis agent. Central nervous system (CNS) cultures were examined by light and electron microscopy as well as standard virological techniques from 3 to 46 days postinfection. The results indicate that although viral
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12. Mifepristone (RU486) protects Purkinje cells from cell death in organotypic slice cultures of postnatal rat and mouse cerebellum
Mifepristone (RU486), which binds with high affinity to both progesterone and glucocorticosteroid receptors (PR and GR), is well known for its use in the termination of unwanted pregnancy, but other activities including neuroprotection have been suggested. Cerebellar organotypic cultures from 3 to 7 postnatal day rat (P3—P7) were studied to examine the
National Academy of Sciences.