Orchestration
Mostrando 37-48 de 49 artigos, teses e dissertações.
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37. Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
The normal reverse transcription of retroviral RNA is a complex process which depends on the orchestration of several steps throughout the virus life cycle. During the assembly of retroviruses, reverse transcriptase (RT) is directed into the virion as a component of the Gag-Pol polyprotein. In the maturation of the Gag-Pol polyprotein of human immunodeficien
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38. PinX1 Is a Novel Microtubule-binding Protein Essential for Accurate Chromosome Segregation*
Mitosis is an orchestration of dynamic interactions between spindle microtubules and chromosomes, which is mediated by protein structures that include the kinetochores, and other protein complexes present on chromosomes. PinX1 is a potent telomerase inhibitor in interphase; however, its function in mitosis is not well documented. Here we show that PinX1 is e
American Society for Biochemistry and Molecular Biology.
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39. Tissue-specific GATA factors are transcriptional effectors of the small GTPase RhoA
Rho-like GTPases play a pivotal role in the orchestration of changes in the actin cytoskeleton in response to receptor stimulation, and have been implicated in transcriptional activation, cell growth regulation, and oncogenic transformation. Recently, a role for RhoA in the regulation of cardiac contractility and hypertrophic cardiomyocyte growth has been su
Cold Spring Harbor Laboratory Press.
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40. An enhancer LEF-1/TCF-1 site is essential for insertion site-independent transgene expression in thymus.
Transcriptional activation of eukaryotic genes involves assembly of specific multiprotein complexes on the promoters and enhancers of the genes. Recently, it has been proposed that the role of some of the proteins in the complex may be architectural, involving DNA bending, orchestration of protein-protein interaction and modulation of nucleosome structure. T
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41. Actin Can Reorganize into Podosomes in Aortic Endothelial Cells, a Process Controlled by Cdc42 and RhoA
Members of the Rho GTPase family play a central role in the orchestration of cytoskeletal rearrangements, which are of prime importance in endothelial cell physiology. To explore their role in this specialized cell type, we used the bacterial toxin cytotoxic necrotizing factor 1 (CNF1) as a Rho GTPase activator. Punctate filamentous actin structures appeared
American Society for Microbiology.
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42. Inactivation of Transcription Factor Gene ACE2 in the Fungal Pathogen Candida glabrata Results in Hypervirulence
During an infection, the coordinated orchestration of many factors by the invading organism is required for disease to be initiated and to progress. The elucidation of the processes involved is critical to the development of a clear understanding of host-pathogen interactions. For Candida species, the inactivation of many fungal attributes has been shown to
American Society for Microbiology.
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43. Orchestration of cooperative events in DNA synthesis and repair mechanism unraveled by transition path sampling of DNA polymerase β's closing
Our application of transition path sampling to a complex biomolecular system in explicit solvent, the closing transition of DNA polymerase β, unravels atomic and energetic details of the conformational change that precedes the chemical reaction of nucleotide incorporation. The computed reaction profile offers detailed mechanistic insights into, as well as k
National Academy of Sciences.
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44. Neurotrophins regulate agrin-induced postsynaptic differentiation
The precise orchestration of synaptic differentiation is critical for efficient information exchange in the nervous system. The nerve–muscle synapse forms in response to agrin, which is secreted from the motor nerve terminal and induces the clustering of acetylcholine receptors (AChRs) and other elements of the postsynaptic apparatus on the subjacent muscl
The National Academy of Sciences.
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45. T-helper-cell cytokines in the early evolution of murine Lyme arthritis.
Genetic susceptibility to murine Lyme arthritis has been correlated with the dominance of T-helper (Th1)- or Th2-cell-associated cytokines. To determine when commitment of the Th cell phenotype occurs, we examined the kinetics of gamma interferon (IFN-gamma) and interleukin-4 (IL-4) production by lymph node T cells of disease-susceptible C3H/HeN and disease-
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46. Combined parenteral and oral immunization results in an enhanced mucosal immunoglobulin A response to Shigella flexneri.
Achieving a vigorous secretory immunoglobulin A (IgA) response in intestinal secretions usually requires multiple doses of antigen given orally, while systemic immunity is more easily attained by parenteral immunization. This study examines the role of combined parenteral and oral immunizations to enhance the early mucosal immune response to an enteropathoge
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47. In vitro expression of tumor necrosis factor-α, interleukin 1β, and interleukin 8 mRNA by bovine macrophages following exposure to Porphyromonas levii
The objective was to evaluate the pro-inflammatory response of bovine macrophages towards Porphyromonas levii, an etiologic agent of acute interdigital phlegmon, by evaluating the mRNA expression of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and interleukin 8 (IL-8). Bovine macrophages detect the presence of bacteria, such as P. levii, and
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48. The dual functions of Fas ligand in the regulation of peripheral CD8+ and CD4+ T cells
Although Fas ligand (FasL) is well characterized for its capacity to deliver a death signal through its receptor Fas, recent work demonstrates that FasL also can receive signals facilitating antigen (Ag)-specific proliferation of CD8+ T cells. The fact that the gld mutation differentially influences the proliferative capacity of CD8+ and CD4+ T cells present
The National Academy of Sciences.