Open Reading Frame Ests
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Transcriptome analysis of Taenia solium cysticerci using Open Reading Frama ESTs (ORESTES)
Background: Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerc
Publicado em: 2010
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2. Seqüências ORESTES (open reading frame expressed sequence tags) de trypanosoma cruzi e transcrição de DNA satélite / ORESTES (open reading frame expressed sequence tags) sequences of Trypanosoma cruzi and transcription of satellite DNA
In T. cruzi databases, aproximately 3.750 ESTs of amastigotes and trypomastigotes, sequenced from the 3´ or 5´ ends cDNA clones can be found in T. cruzi databases. The ORESTES (Open Reading Frame Expressed Sequence Tags) methodology generates partial transcribed sequences derived mainly from the central portions of mRNAs, favoring the discovery of new gene
Publicado em: 2008
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3. Identificação e análise de etiquetas de seqüências expressas (ESTs) na hipófise e hipotálamo de Gallus gallus / Identification and characterization of expressed sequence tags (ESTs) in pituitary and hypothalamus in Gallus gallus
The brazilian aviculture has reached a high development level in both meat and egg production as a result of constant technological atualization in the sector. Biotechnology can contribute in this way acting in selection programs of animals that show higher growth and development potential. The global animal physiology is direct or indirectly controlled by p
Publicado em: 2007
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4. Avaliação da capacidade de genes de Eucaliptus grandis em conferir tolerância à deficiência hídrica
Uma cuidadosa revisão bibliográfica, seguida por uma pesquisa in silico no banco de dados de expressed sequence tags (ESTs) do projeto GENOLYPTUS – Rede Brasileira de Pesquisa do Genoma de Eucalyptus, foram realizadas visando identificar classes de genes potencialmente relacionadas com a resposta vegetal à deficiência hídrica em Eucalyptus grandis. Os
Publicado em: 2007
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5. CML transcriptome analysis using ORESTES (Open Reading Frame Expression Sequence Tags) / Avaliação do transcriptoma da leucemia mieloide cronica por ORESTES (Open Reading Frame Expression Sequence Tags)
The complete collection of transcripts generated from the human genome cannot be predicted from the genome sequence, but should be directly determined for each tissue, due to variations of gene expression in different tissues and disease states, and because genes can encode multiple transcripts derived from alternate splicing and polyadenylation sites. As pa
Publicado em: 2003
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6. The use of Open Reading frame ESTs (ORESTES) for analysis of the honey bee transcriptome
BioMed Central.
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7. Comparative Plant Genomics Resources at PlantGDB1
PlantGDB (http://www.plantgdb.org/) is a database of plant molecular sequences. Expressed sequence tag (EST) sequences are assembled into contigs that represent tentative unique genes. EST contigs are functionally annotated with information derived from known protein sequences that are highly similar to the putative translation products. Tentative Gene Ontol
American Society of Plant Biologists.
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8. The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome
Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency
The National Academy of Sciences.
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9. Molecular cloning and functional analysis of a human cDNA encoding an Escherichia coli AlkB homolog, a protein involved in DNA alkylation damage repair.
The Escherichia coli AlkB protein is involved in protecting cells against mutation and cell death induced specifically by SN2-type alkylating agents such as methyl methanesulfonate (MMS). A human cDNA encoding a polypeptide homologous to E.coli AlkB was discovered by searching a database of expressed sequence tags (ESTs) derived from high throughput cDNA seq
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10. Gene prediction and verification in a compact genome with numerous small introns
The genomes of clusters of related eukaryotes are now being sequenced at an increasing rate, creating a need for accurate, low-cost annotation of exon–intron structures. In this paper, we demonstrate that reverse transcription-polymerase chain reaction (RT–PCR) and direct sequencing based on predicted gene structures satisfy this need, at least for singl
Cold Spring Harbor Laboratory Press.