Oligonucleotide
Mostrando 1-12 de 4824 artigos, teses e dissertações.
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1. Gene Silencing Therapeutics in Cardiology: A Review Article
Abstract Therapeutics that inhibit enzymes, receptors, ion channels, and cotransporters have long been the mainstay of cardiovascular medicine. Now, oligonucleotide therapeutics offer a modern variation on this paradigm of protein inhibition. Rather than target a protein, however, small interfering ribonucleic acids and antisense oligonucleotides target the
International Journal of Cardiovascular Sciences. Publicado em: 2022
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2. Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
This paper reports the DNA interaction and cytotoxicity of binary and ternary copper(II) complexes of valproic acid with phenanthroline or bipyridine and the complementary characterization of Cu(Valp)2Bipy by crystallography. Circular dichroism, plasmid and oligonucleotide assays have shown that the complexes interact with DNA in different manners and are ab
J. Braz. Chem. Soc.. Publicado em: 2019-03
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3. Allele and haplotype frequencies of human platelet and leukocyte antigens in platelet donors
RESUMO Objetivo Descrever as frequências alélicas e haplotípicas de genes dos antígenos leucocitários humanos nos loci -A,- B e dos antígenos plaquetários humanos para os sistemas HPA-1 a 9, 11 e 15. Métodos Foram incluídos 867 doadores voluntários, saudáveis, não relacionados, que doaram plaquetas por aférese entre janeiro de 2011 e dezembro
Einstein (São Paulo). Publicado em: 07/02/2019
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4. Protocol for chromosome-specific probe construction using PRINS, micromanipulation and DOP-PCR techniques
ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cu
An. Acad. Bras. Ciênc.. Publicado em: 07/12/2017
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5. Salmonella Enteritidis in Layer Farms of Different Sizes Located in Northern China: On-Farm Sampling and Detection by the PCR Method
ABSTRACT Salmonella enterica subspecies enterica serotype Enteritidis (SE) has caused foodborne infections over decades. It is transmitted mainly from contaminated eggs to humans. SE is commonly present in layer houses, and closely interacts with environmental factors. The objective of the present study was to develop a viable PCR method to identify SE in en
Rev. Bras. Cienc. Avic.. Publicado em: 2017-09
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6. Human leukocyte antigen allele linkage disequilibrium and haplotype structure in volunteer bone marrow donors of Paraná State
Abstract Background Bone marrow transplantation has been used in the treatment of various diseases, especially hematologic diseases. The success of this treatment, among other factors, requires human leukocyte antigens (HLA) compatibility between patient and donor. Knowing the human leukocyte antigens allele group and haplotype frequencies as well as the li
Rev. Bras. Hematol. Hemoter.. Publicado em: 2017-09
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7. Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase
BACKGROUND Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02
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8. BIOMATERIAIS PARA FORMULAÇÕES DE BASE NANOTECNOLÓGICA VISANDO TERAPIA GENÉTICA OCULAR
Gene therapy is a promising tool for the treatment of ocular diseases. Nevertheless, there are some import limitations, especially related to the ocular route and the characteristics of nucleic acids that must be overcome to ensure the success of the therapy. In this context, nanotechnology-based carriers such as nanoemulsions, nanoparticles, dendrimers and
Quím. Nova. Publicado em: 2017-01
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9. Investigation of the Interaction of 2-(2'-Hydroxyphenyl)-benzoxazoles and their Derivatives with B-DNA by Docking and Molecular Dynamics
In this work we carried out a study covering conformational analysis, docking calculations and molecular dynamics (MD) simulations of six excited state intramolecular proton transfer (ESIPT)-fluorescent 2-(2'-hydroxyphenyl)-benzoxazoles, interacting with the Dickerson-Drew (d(CGCGAATTCGCG)2) dodecamer in B-DNA conformation. In the analysis of the molecular d
J. Braz. Chem. Soc.. Publicado em: 2015-03
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10. Genome-wide transcription analyses in Mycobacterium tuberculosis treated with lupulone
Mycobacterium tuberculosis (M. tuberculosis), the causative agent of tuberculosis, still causes higher mortality than any other bacterial pathogen until now. With the emergence and spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR-TB) strains, it becomes more important to search for alternative targets to develop new antimycobacterial d
Braz. J. Microbiol.. Publicado em: 18/04/2014
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11. In vitro detection of pathogenic Listeria monocytogenes from food sources by conventional, molecular and cell culture method
Among current in vitro methods for identification of pathogenic Listeria monocytogenes (L. monocytogenes) rely on growth in culture media, followed by isolation, and biochemical and serological identification. Now PCR (Polymerase Chain Reaction) has been used for the rapid, sensitive and specific detection of pathogenic L. monocytogenes. The pathogenicity of
Braz. J. Microbiol.. Publicado em: 2013-09
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12. O papel modulador do receptor símile a TOLL 2 (TLR2) e da microbiota intestinal na sensibilidade e sinalização da insulina em camundongos / The modulatory role of the Toll-like receptor (TLR)2 and of the gut microbiota in the modulation of the insulin sensitivity and signaling
Environmental factors and host genetics interact to control the gut microbiota, which may have a role in the development of obesity and insulin resistance. TLR2 deficient mice, under germ-free conditions are protected from diet-induced insulin resistance. Diet-induced obese mice, acutely treated with TLR2 oligonucleotide antisense during 4 days showed increa
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/02/2012