Oleosin
Mostrando 1-12 de 12 artigos, teses e dissertações.
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1. Transgenic Expression and Identification of Recombinant Human Proinsulin in Peanut
The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery methods that rely on higher doses, is expected to result in a substantial escalation in the future demand for affordable insulin. Plant-based systems offer a safe and economical method for producing pharmaceutical proteins. We used peanut (Arachis hypogaea L.)
Braz. arch. biol. technol.. Publicado em: 24/05/2016
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2. Desenvolvimento de estratégia para extrusão de lipídios por engenharia genética, subsequente à análise genética da via exocítica da microalga Neochloris oleoabundans / Development of genetic engineering atrategy of lipids extrusior following the genetic analysis of exocytic pathway from micralga Neochloris oleoabudans
Há no mundo um consenso sobre a necessidade de se encontrar fontes de carbono renováveis para a substituição do petróleo. O cultivo de microalgas tem sido considerado uma alternativa promissora para suprir essas cadeias carbônicas e biocombustíveis, visto que são microorganismos fotossintetizantes que podem acumular grandes quantidades de triacilglic
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/12/2011
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3. Maize oleosin is correctly targeted to seed oil bodies in Brassica napus transformed with the maize oleosin gene.
Oleosins are small hydrophobic abundant proteins localized in the oil bodies of plant seeds. An oleosin gene from the monocotyledonous maize (Zea mays L.) was transferred into the dicotyledonous Brassica napus L. using Agrobacterium-mediated transformation. The maize oleosin gene was placed under the control of either its own promoter/terminator or the promo
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4. Role of the proline knot motif in oleosin endoplasmic reticulum topology and oil body targeting.
An Arabidopsis oleosin was used as a model to study oleosin topology and targeting to oil bodies. Oleosin mRNA was in vitro translated with canine microsomes in a range of truncated forms. This allowed proteinase K mapping of the membrane topology. Oleosin maintains a conformation with a membrane-integrated hydrophobic domain flanked by N- and C-terminal dom
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5. Cotranslational Integration of Soybean (Glycine max) Oil Body Membrane Protein Oleosin into Microsomal Membranes.
Storage triglycerides in oil seeds are sequestered in discrete organelles termed oil bodies. They are bounded by a monolayer of phospholipids in which a few distinct proteins (oleosins) are embedded. Synthesis of soybean (Glycine max) 24-kD oleosin was analyzed by in vitro transcription and translation in reticulocyte lysate in the presence of canine microso
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6. Induction of Lipid and Oleosin Biosynthesis by (+)-Abscisic Acid and Its Metabolites in Microspore-Derived Embryos of Brassica napus L.cv Reston (Biological Responses in the Presence of 8[prime]-Hydroxyabscisic Acid).
Microspore-derived (MD) embryos of Brassica napus L. cv Reston were used to test the effects of (+)-abscisic acid ([(+)-ABA]) and its metabolites, 8[prime]-hydroxyabscisic acid (8[prime]-OH ABA) and (-)-phaseic acid (PA), on the accumulation of very long-chain monounsaturated fatty acids (VLCMFAs) and induction of genes encoding a 19-kD oleosin protein and a
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7. Embryo-Specific Gene Expression in Microspore-Derived Embryos of Brassica napus. An Interaction between Abscisic Acid and Jasmonic Acid1, 2
The induction of napin and oleosin gene expression in Brassica napus microspore-derived embryos (MDEs) was studied to assess the possible interaction between abscisic acid (ABA) and jasmonic acid (JA). Napin and oleosin transcripts were detected sooner following treatment with ABA than JA. Treatment of MDEs with ABA plus JA gave an additive accumulatio
American Society of Plant Physiologists.
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8. Structural requirements of oleosin domains for subcellular targeting to the oil body.
We have investigated the protein domains responsible for the correct subcellular targeting of plant seed oleosins. We have attempted to study this targeting in vivo using "tagged" oleosins in transgenic plants. Different constructs were prepared lacking gene sequences encoding one of three structural domains of natural oleosins. Each was fused in frame to th
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9. Cotton (Gossypium hirsutum) MatP6 and MatP7 oleosin genes.
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10. Steroleosin, a Sterol-Binding Dehydrogenase in Seed Oil Bodies1
Besides abundant oleosin, three minor proteins, Sop 1, 2, and 3, are present in sesame (Sesamum indicum) oil bodies. The gene encoding Sop1, named caleosin for its calcium-binding capacity, has recently been cloned. In this study, Sop2 gene was obtained by immunoscreening, and it was subsequently confirmed by amino acid partial sequencing and immunological r
American Society of Plant Physiologists.
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11. Characterization of oleosins in the pollen coat of Brassica oleracea.
Mature Brassica oleracea pollen grains are covered with a lipophilic pollen coat containing a variety of proteins. Screening of an anther cDNA expression library for the coding sequences of such proteins resulted in the isolation of a number of cDNA clones encoding glycine-rich oleosins. The proteins were shown to be attached to the lipophilic coat material
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12. Competitive Inhibition of Abscisic Acid-Regulated Gene Expression by Stereoisomeric Acetylenic Analogs of Abscisic Acid.
The properties of two enantiomeric synthetic acetylenic abscisic acid (ABA) analogs (PBI-51 and PBI-63) in relation to ABA-sensitive gene expression are reported. Using microspore-derived embryos of Brassica napus as the biological material and their responsiveness to ABA in the expression of genes encoding storage proteins as a quantitative bioassay, we mea