Nuclear Transfer
Mostrando 13-24 de 552 artigos, teses e dissertações.
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13. Simplification of Lipase Design in the Enzymatic Kinetic Resolution of Amines by Saturation Transfer Difference NMR
In this work, we demonstrate a nuclear magnetic resonance (NMR) method for racemic amide and lipase interaction as a first-pass design method in the enzymatic kinetic resolution of amines. As a novel adaptation of commonly used protein-ligand screening NMR methodologies, this approach relies upon a lipase-amide interaction wherein the time-consuming is reduc
J. Braz. Chem. Soc.. Publicado em: 2016-10
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14. Synthesis and Photophysical Characterization of Novel ESIPT Triazinyl-Benzazole Derivatives
In this work, the synthesis, characterization and photophysical study of new derivatives of triazinyl-benzazoles with fluorescence by excited-state intramolecular proton transfer (ESIPT) are presented. It regards the synthesis of cyanuric chloride with different 2-(2’-hydroxyphenyl) benzazoles, two quite attractive groups from the synthetic and technologic
J. Braz. Chem. Soc.. Publicado em: 2015-12
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15. The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differe
Genet. Mol. Biol.. Publicado em: 01/05/2015
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16. Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo
The compounds 6-dimethylaminopurine and cycloheximide promote the successful production of cloned mammals and have been used in the development of embryos produced by somatic cell nuclear transfer. This study investigated the effects of 6-dimethylaminopurine and cycloheximide in vitro, using the thiazolyl blue tetrazolium bromide colorimetric assay to assess
Braz J Med Biol Res. Publicado em: 28/03/2014
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17. Estudos biofísicos de chaperonas de secreção e de interações proteína-ligante / Biophysical studies on secretion chaperones and protein-ligand interactions
So far, the Xanthomonas axonopodis pv. citri (XAC) mechanisms of bacterial virulence is unknown. It is believed that secretion chaperones (CS) are involved in the XAC´s virulence process by first forming complexes with virulence factors, and assisting in their presentation to corresponding secretion systems using ATP as a source of energy. Fluorescence emis
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2012
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18. Use of chromatin modifying agents in bovine somatic cell nuclear transfer / Uso de agentes modificadores de cromatina na transferência nuclear de células somáticas em bovinos
Embora a transferência nuclear de células somáticas (TNCS) seja uma ferramenta promissora, seu amplo uso é impedido devido às altas taxas de mortalidade durante o desenvolvimento dos animais clonados. Acredita-se que a reprogramação epigenética anormal seja a principal causa desta baixa eficiência. Nós hipotetizamos que agentes modificadores de cro
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/12/2011
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19. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes.
Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by som
Genetics and Molecular Research. Publicado em: 2011
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20. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with io
Animal Reproduction Science. Publicado em: 2011
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21. Desenvolvimento e implementação de um novo sistema pneumático de transferência para irradiação de materiais no reator IEA-R1 / Development and implementation of a new pneumatic transfer system for materials irradiation at IEA-R1 reactor
Pneumatic Transfer Systems (PTS) are classified as mechanical equipment largely operated all over the world for transport of a huge sort of objects, samples and materials located at nearly terminals or even at separated ones. System applicability is often recognized in many activities, such as medicine (hospital settings, clinical analysis labs), industry (s
Publicado em: 2011
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22. Transferência nuclear de células somáticas (TNCS) em ruminantes: aspectos moleculares e produção de embriões. / Somatic cell nuclear transfer (SCNT) in ruminants: the molecular and embryo production.
O sucesso da produção de animais clones por Transferência Nuclear de Células Somáticas (TNCS) envolve múltiplas etapas e cada uma destas pode influenciar a eficiência final da técnica. Assim, para investigar fatores relacionados à TNCS em ruminantes, esse trabalho teve como objetivos: (i) avaliar o efeito de um protocolo alternativo de estimulação
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/12/2010
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23. Utilização de redes neurais artificiais para determinar o tempo de resposta de sensores de temperatura do tipo RTD / Time response of temperature sensors using neural networks
In a PWR nuclear power plant, the primary coolant temperature and feedwater temperature are measured using RTDs (Resistance Temperature Detectors). These RTDs typically feed the plants control and safety systems and must, therefore, be very accurate and have good dynamic performance. The response time of RTDs is characterized by a single parameter called the
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 16/09/2010
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24. Thermal and epithermal neutron fluence rates in the irradiation facilities of the TRIGA IPR-R1 nuclear reactor
The TRIGA IPR-R1 nuclear reactor at the Centro de Desenvolvimento da Tecnologia Nuclear CDTN/CNEN, Belo Horizonte, Brazil, has three irradiation facilities: a rotary specimen rack outside the reactor core with 40 irradiation channels which provide large scale isotope irradiation; the central tube placed at the center of the reactor core which permits sample
Brazilian Journal of Physics. Publicado em: 2010-03