Nuclear Reprogramming
Mostrando 1-12 de 29 artigos, teses e dissertações.
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1. New tools for cell reprogramming and conversion: Possible applications to livestock
Abstract Somatic cell nuclear transfer and iPS are both forms of radical cell reprogramming able to transform a fully differentiated cell type into a totipotent or pluripotent cell. Both processes, however, are hampered by low efficiency and, in the case of iPS, the application to livestock species is uncertain. Epigenetic manipulation has recently emerged a
Anim. Reprod.. Publicado em: 28/11/2019
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2. Reprogramação de células mesenquimais de tecido adiposo em células-tronco pluripotentes por meio de proteína de fusão TAT / Nuclear reprogramming of adipose-tissue mesenchymal stem cells into pluripotent stem cells using TAT fusion protein
Os vírus são eficazes na transferência de genes em células devido aos seus mecanismos especializados. No entanto, vírus como veículos de entrega de genes podem acarretar em problemas, particularmente quando proposto para reprogramar células somáticas em células-tronco pluripotentes induzidas (iPS) visando utilização terapêutica. No presente estud
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/02/2012
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3. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes.
Cloning by nuclear transfer is often associated with poor results due to abnormal nuclear reprogramming of somatic donor cells and altered gene expression patterns. We investigated the expression patterns of imprinted genes IGF2 and IGF2R in 33- to 36-day bovine embryos and chorio-allantoic membranes derived from in vivo- and in vitro-produced embryos by som
Genetics and Molecular Research. Publicado em: 2011
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4. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with io
Animal Reproduction Science. Publicado em: 2011
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5. Padrão de metilação da DMR do último éxon do gene IGF2 em ovócitos e células do cumulus de vacas nelore
Assisted reproductive biotechnologies are essential instrument for the modern cattle breeding. In vitro embryo production (IVP) is prominence, situating Brazil as a country that more produce and transfer in vitro embryos in the word. In spite of the IVP big capacity to produce embryos per animal, this production can be better, being the oocyte quality the mo
Publicado em: 2009
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6. PHYLOGENETICS AND MORPHOLOGICAL EVOLUTION OF SCLERACTINIAN CORALS
Scleractinian corals are modular organisms of great ecological and economic importance that may have solitary or colonial growth forms. In spite of the rich fossil record, the evolutionary history of the group is poorly understood and its taxonomy is highly artificial because many of the features commonly employed in systematic studies of the group exhibit l
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 12/11/2007
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7. Passagem celular, sexo e transcrição X-específica interferem no desenvolvimento embrionário e fetal de bovinos produzidos por transferência nuclear / Celular passage, sex and X-specific transcription interfere in bovine nuclear transfer embryo and fetal development
Cells cultured for long-term periods accumulate genetic and epigenetic modifications that result in improper nuclear reprogramming of somatic cell nuclear transfer (SCNT) embryos. Furthermore, the sex may be a limiting factor in blastocysts production and in post-implantation developmental competence. Therefore, the objective of this study was to determine t
Publicado em: 2007
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8. Nuclear reprogramming and stem cell creation
The transplantation of a somatic cell nucleus to an enucleated egg results in a major reprogramming of gene expression and switch in cell fate. We review the efficiency of nuclear reprogramming by nuclear transfer. The serial transplantation of nuclei from defective first-transfer embryos and the grafting of cells from such embryos to normal host embryos gre
National Academy of Sciences.
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9. Nuclear reprogramming in heterokaryons is rapid, extensive, and bidirectional
An understanding of nuclear reprogramming is fundamental to the use of cells in regenerative medicine. Due to technological obstacles, the time course and extent of reprogramming of cells following fusion has not been assessed to date. Here, we show that hundreds of genes are activated or repressed within hours of fusion of human keratinocytes and mouse musc
The Federation of American Societies for Experimental Biology.
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10. Nuclear transfer: Progress and quandaries
Cloning mammals by nuclear transfer is a powerful technique that is quickly advancing the development of genetically defined animal models. However, the overall efficiency of nuclear transfer is still very low and several hurdles remain before the power of this technique will be fully harnessed. Among these hurdles include an incomplete understanding of biol
BioMed Central.
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11. Cloning animals by somatic cell nuclear transfer – biological factors
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced. High abortion and fetal mortality rates are commonly observed. These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by
BioMed Central.
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12. Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos
Mouse embryos undergo genome-wide methylation reprogramming by demethylation in early preimplantation development, followed by remethylation thereafter. Here we show that genome-wide reprogramming is conserved in several mammalian species and ask whether it also occurs in embryos cloned with the use of highly methylated somatic donor nuclei. Normal bovine, r
The National Academy of Sciences.