Ns5b Hcv Region
Mostrando 1-12 de 71 artigos, teses e dissertações.
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1. [PROVISIONAL] Resistance mutations of NS3 and NS5b in treatment-naïve patients infected with Hepatitis C virus in Santa Catarina and Rio Grande do Sul States, Brazil
Abstract Hepatitis C virus (HCV) infection is a worldwide health problem. Nowadays, direct-acting antiviral agents (DAAs) are the main treatment; however, the high level of variability of HCV lead to development of resistance-associated variants (RAVs). Thus, look into the RAVs among infected patients is an important tool for monitoring the efficacy of the t
Genet. Mol. Biol.. Publicado em: 21/10/2019
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2. Investigação epidemiológica e molecular da infecção pelo vírus da hepatite C em usuários de drogas ilícitas no Brasil Central / Epidemiologic and molecular investigation of hepatitis C virus infection among illicit drug users in Central Brazil
Hepatitis C virus (HCV) infection is an important problem of public health. Drug users (DU) constitute a group of frequent exposure to HCV and little is known about this infection in DU in Brazil. This study aimed to investigate the seroepidemiological and molecular profile of HCV infection among drug users in Central Brazil. A total of 691 DU, being 102 inj
Publicado em: 2009
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3. Genetic variability in the 5' UTR and NS5A regions of hepatitis C virus RNA isolated from non-responding and responding patients with chronic HCV genotype 1 infection
Sequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and reflects the modes and intensities of selection mechanisms operating on the virus. In this work, we sought to investigate using classical population genetics parameters, the genetic variability of HCV genotype 1 using the 5' UTR and NS5A regions from treatment n
Memórias do Instituto Oswaldo Cruz. Publicado em: 2008-09
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4. Genotyping hepatitis C virus from hemodialysis patients in Central Brazil by line probe assay and sequence analysis
The present study examined the distribution of hepatitis C virus (HCV) genotypes and subtypes in a hemodialysis population in Goiás State, Central Brazil, and evaluated the efficiency of two genotyping methods: line probe assay (LiPA) based on the 5' noncoding region and nucleotide sequencing of the nonstructural 5B (NS5B) region of the genome. A total of 1
Brazilian Journal of Medical and Biological Research. Publicado em: 2007-04
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5. Caracterização molecular do vírus da hepatite c em indivíduos co-infectados com HIV-1
Human immunodeficiency virus (HIV-1) and Hepatitis C virus (HCV) are considered problems of public health with about 40 millions infected by HIV-1 and 170 millions infected by HCV worldwide. The co-infection HIV-1/HCV is common in individuals exposed to percutaneous pathways and the hepatitis C has been emerging as the main cause of death in HIV-1 positive i
Publicado em: 2007
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6. Molecular evidences for horizontal transmission of HCV inside couples / Evidências moleculares da transmissão horizontal do vírus da hepatite C (VHC) entre cônjuges
HCV transmission has decreased with the adoption of universal blood donors screening and social policies to reduce risk of infection in IVDU, but HCV is still a worldwide health problem. The epidemiological route of infection cannot be identified in a significant proportion of patients. Some studies demonstrated the presence of viral RNA in different secreti
Publicado em: 2006
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7. "Hepatite C: transmissão entre casais" / Hepatitis C: transmission between couples.
RESUMO Cavalheiro, NP. Hepatite C: transmissão entre casais. São Paulo, 2004. 111p. Tese (Doutorado) - Faculdade de Medicina, Universidade de São Paulo. Introdução: A ocorrência e eficiência da transmissão sexual do VHC na ausência de outros fatores de risco ainda é muito controversa. Foram investigados e analisados 24 casais, ambos cônjuges infec
Publicado em: 2004
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8. Virus-Specific Cofactor Requirement and Chimeric Hepatitis C Virus/GB Virus B Nonstructural Protein 3
GB virus B (GBV-B) is closely related to hepatitis C virus (HCV) and causes acute hepatitis in tamarins (Saguinus species), making it an attractive surrogate virus for in vivo testing of anti-HCV inhibitors in a small monkey model. It has been reported that the nonstructural protein 3 (NS3) serine protease of GBV-B shares similar substrate specificity with i
American Society for Microbiology.
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9. Identification of a C-Terminal Regulatory Motif in Hepatitis C Virus RNA-Dependent RNA Polymerase: Structural and Biochemical Analysis
The NS5B RNA-dependent RNA polymerase encoded by the hepatitis C virus (HCV) is a key component of the viral replicase. Reported here is the three-dimensional structure of HCV NS5B polymerase, with the highlight on its C-terminal folding, determined by X-ray crystallography at 2.1-Å resolution. Structural analysis revealed that a stretch of C-terminal resid
American Society for Microbiology.
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10. A Natural Intergenotypic Recombinant of Hepatitis C Virus Identified in St. Petersburg
Hepatitis C virus (HCV) evolution is thought to proceed by mutations within the six genotypes. Here, we report on a viable spontaneous HCV recombinant and we show that recombination may play a role in the evolution of this virus. Previously, 149 HCV strains from St. Petersburg had been subtyped by limited sequencing within the NS5B region. In the present stu
American Society for Microbiology.
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11. Specific Interaction between the Hepatitis C Virus NS5B RNA Polymerase and the 3′ End of the Viral RNA
Hepatitis C virus (HCV) NS5B protein is the viral RNA-dependent RNA polymerase capable of directing RNA synthesis. In this study, an electrophoretic mobility shift assay demonstrated the interaction between a partially purified recombinant NS5B protein and a 3′ viral genomic RNA with or without the conserved 98-nucleotide tail. The NS5B-RNA complexes were
American Society for Microbiology.
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12. Slow Binding Inhibition and Mechanism of Resistance of Non-nucleoside Polymerase Inhibitors of Hepatitis C Virus
The binding affinity of four palm and thumb site representative non-nucleoside inhibitors (NNIs) of HCV polymerase NS5B to wild-type and resistant NS5B polymerase proteins was determined, and the influence of RNA binding on NNI binding affinity was investigated. NNIs with high binding affinity potently inhibited HCV RNA polymerase activity and replicon repli
American Society for Biochemistry and Molecular Biology.