Non Viral Vectors
Mostrando 1-12 de 41 artigos, teses e dissertações.
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1. Cholesterol improves the transfection efficiency of polyallylamine as a non-viral gene delivery vector
ABSTRACT Cationic polymers such as polyallylamine (PAA) having primary amino groups are poor transfection agents and possess a high cytotoxicity index when used without any chemical modification. In this study, PAA was modified with cholesterol in order to improve transfection efficiency and to reduce cytotoxicity. PAA polymers with molecular weights of 15 a
Braz. J. Pharm. Sci.. Publicado em: 26/10/2017
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2. Evaluation of arboviruses of public health interest in free-living non-human primates (Alouatta spp., Callithrix spp., Sapajus spp.) in Brazil
INTRODUCTION: The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Paraná and Mato Grosso do Sul, Brazil. METHODS: NHP sera samples (total n = 80, Alouatta spp. n = 07, Callithrix spp. n = 29 a
Rev. Soc. Bras. Med. Trop.. Publicado em: 2015-04
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3. Desenvolvimento de vetores não virais para entrega gênica baseados na cadeia leve de dineína Rp3 = : Development of non viral vectors for gene delivery based on dynein light chain Rp3 / Development of non viral vectors for gene delivery based on dynein light chain Rp3
Gene delivery is a promising technique with great medical potential that consists in the introduction of exogenous nucleic acids, and can be applied for gene therapy as well as DNA vaccination. However, its use is still limited by the lack of an ideal delivery vector, which is both safe and efficient. Although much more effective, viral vectors still raise s
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/07/2012
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4. Recombinant vaccines and the development of new vaccine strategies
Vaccines were initially developed on an empirical basis, relying mostly on attenuation or inactivation of pathogens. Advances in immunology, molecular biology, biochemistry, genomics, and proteomics have added new perspectives to the vaccinology field. The use of recombinant proteins allows the targeting of immune responses focused against few protective ant
Braz J Med Biol Res. Publicado em: 2012-12
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5. Desenvolvimento de nanosistemas farmacêuticos para terapia gênica
A terapia gênica é um dos maiores desafios propostos pela pesquisa pós-genômica e se baseia na transferência de material genético a uma célula, tecido ou órgão com o intuito de curar ou melhorar o estado clínico do paciente. Em sua forma mais simples, a terapia gênica consiste na inserção de genes funcionais em células com genes defeituosos obj
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 14/03/2011
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6. Detecção do vírus da diarreia viral bovina em carrapatos Rhipicephalus (Boophilus) microplus alimentados em bovino persistentemente infectado= Detection of bovine viral diarrhea virus in Rhipicephalus (Boophilus) microplus ticks fed on persistently infected cattle / Detection of bovine viral diarrhea virus in Rhipicephalus (Boophilus) microplus ticks fed on persistently infected cattle
Background: Bovine viral diarrhea virus (BVDV) is one of the main agents that cause economical losses in cattle worldwide. Congenitally infected calves that are born persistently infected (PI) to BVDV are the main sources of infection to susceptible cattle. Direct contact is the most important form of transmission, but indirect contact can also spread BVDV,
Publicado em: 2011
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7. Detecção do virus da diarréia bovina em carrapatos Rhipicephalus (boophilus) microplus alimentados em bovino persistentemente infectado
Background: Bovine viral diarrhea virus (BVDV) is one of the main agents that cause economical losses in cattle worldwide. Congenitally infected calves that are born persistently infected (PI) to BVDV are the main sources of infection to susceptible cattle. Direct contact is the most important form of transmission, but indirect contact can also spread BVDV,
Publicado em: 2011
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8. Modulação da apresentação antigênica por células dendríticas derivadas de monócitos utilizando diferentes produtos virais do HIV: potencial de utilização em vacina terapêutica. / Modulation of monocyte-derived dendritic cell antigen presentation using different HIV antigenic: potential use in therapeutic vaccine.
Despite more than 20 years of effort, the design of an effective HIV-1 vaccine remains an enormous challenge. In this scenario, new immunological approaches must be considered. Monocytes from HIV-serodiscordant couples and non HIV exposed controls were differentiated in vitro into dendritic cells (MoDC), pulsed with different virus antigens and cultured with
Publicado em: 2010
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9. Transient high-level expression of B-galactosidase after transfection of fibroplasts from GM1 gangliosidosis patients with plasmid DNA
GM1 gangliosidosis is an autosomal recessive disorder caused by the deficiency of lysosomal acid hydrolase ß-galactosidase (ß-Gal). It is one of the most frequent lysosomal storage disorders in Brazil, with an estimated frequency of 1:17,000. The enzyme is secreted and can be captured by deficient cells and targeted to the lysosomes. There is no effective
Publicado em: 2010
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10. Transient high-level expression of ß-galactosidase after transfection of fibroblasts from GM1 gangliosidosis patients with plasmid DNA
GM1 gangliosidosis is an autosomal recessive disorder caused by the deficiency of lysosomal acid hydrolase ß-galactosidase (ß-Gal). It is one of the most frequent lysosomal storage disorders in Brazil, with an estimated frequency of 1:17,000. The enzyme is secreted and can be captured by deficient cells and targeted to the lysosomes. There is no effective
Brazilian Journal of Medical and Biological Research. Publicado em: 2008-04
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11. Expressão da proteína do envelope do vírus da Febre Amarela em células de inseto
Yellow fever is an haemorrhagic disease caused by a virus that belongs to the genus Flavivirus (Flaviviridae family) and is transmitted by mosquitoes. It is a positive- sense, single-stranded, enveloped RNA virus, and its genome consists of 10,862 nucleotides coding for a single ORF of 10,233 nucleotides. This ORF encodes three structural proteins (capsid, p
Publicado em: 2007
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12. Development of artificial replicative plasmids for transformation of Mycoplasma pulmonis, M. capricolum and M. mycoïdes subsp. mycoïdes, and disruption of the M. pulmonis hemolysin A gene by homologous recombination / Desenvolvimento de plasmídeos replicativos artificiais para transformação de Mycoplasma pulmonis, M. capricolum e M. mycoïdes subsp. mycoïdes, e dirupção do gene da hemolisina A de M. pulmonis por recombinação homóloga
Mycoplasmas are the smallest microorganisms capable of self replication known to date, responsible for many diseases in man and animals, infecting also plants and insects. They constitute a large group of bacteria, classified in different genera in the class Mollicutes, which main common characteristic, besides the small genome, is the absence of a cell wall
Publicado em: 2002