Neurotransmitter Uptake
Mostrando 1-12 de 62 artigos, teses e dissertações.
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1. GABA and glutamate transporters: new events and function in the vertebrate retina
The neural retina is a highly complex tissue composed of excitatory and inhibitory neurons and glial cells. Glutamate, the main excitatory neurotransmitter, mediates information transfer from photoreceptors, bipolar cells, and ganglion cells, whereas interneurons, mainly amacrine and horizontal cells, use γ-aminobutyric acid (GABA), the main inhibitory neur
Psychol. Neurosci.. Publicado em: 2013
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2. Isolation and structural and functional characterization of Ts15, a new neurotoxin from the venom of the scorpion Tityus serrulatus / Isolamento e caracterização estrutural e funcional da Ts15, uma nova neurotoxina da peçonha do escorpião Tityus serrulatus
Os escorpiões são um dos grupos de animais mais antigos da Terra. Eles são artrópodes e pertecem a classe Arachinida e Ordem Scorpionida. A família Buthidae compreende as espécies responsáveis pelos acidentes graves em humanos, incluindo a espécie Tityus serrulatus, o maior responsável por esses acidentes no Brasil. A peçonha do T. serrulatus cont�
Publicado em: 2010
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3. Estudo histolÃgico e morfomÃtrico do disco epifisÃrio de ratos neonatos tratados com fluoxetina
The selective inhibitors of the re-uptake of the serotonin acts increasing the availability of this neurotransmitter in the synaptic cleft. Recent studies demonstrated the presence of receptors of serotonin in bone cells, suggesting the participation of this substance in the bone metabolism. The aim of this study is to verify the effects of the use of fluoxe
Publicado em: 2004
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4. Selective loss of noradrenergic phenotypic characters in neuroblasts of the rat embryo.
To define the fate of embryonic neuroblasts in rat gut, which transiently express several noradrenergic traits, we investigated the high-affinity uptake of norepinephrine. At 12.5 days of gestation, these cells exhibited immunoreactivity to tyrosine hydroxylase [tyrosine 3-monoxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating),
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5. Expression of neurotransmitter transport from rat brain mRNA in Xenopus laevis oocytes.
To permit a molecular characterization of neurotransmitter transporter proteins, we have studied uptake activities induced in Xenopus laevis oocytes after injection of adult rat forebrain, cerebellum, brainstem, and spinal cord poly(A)+ RNA. L-Glutamate uptake could be observed as early as 24 hr after injection, was linearly related to the quantity of mRNA i
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6. Characterization of a genetically reconstituted high-affinity system for serotonin transport.
By transfecting mouse fibroblast L-M cells with human genomic DNA, we have established and identified several clonal cell lines that stably express a high-affinity serotonin (5-HT)-uptake mechanism absent in untransfected host cells. One such cell line, L-S1, possesses features of 5-[3H]HT uptake similar to those previously characterized in the central nervo
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7. Ancestry and progeny of nutrient amino acid transporters
The biosynthesis of structural and signaling molecules depends on intracellular concentrations of essential amino acids, which are maintained by a specific system of plasma membrane transporters. We identify a unique population of nutrient amino acid transporters (NATs) within the sodium-neurotransmitter symporter family and have characterized a member of th
National Academy of Sciences.
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8. gamma-Aminobutyric acid uptake by a bacterial system with neurotransmitter binding characteristics.
gamma-Aminobutyric acid (GABA), an amino acid, has been found in every class of living organisms. In higher organisms, GABA is a neurotransmitter and binds with high affinity and specificity to GABA receptors on neurons in a sodium-independent reaction that is saturable. The role of GABA in organisms lacking nervous tissue is not known. This report describes
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9. Stimulation of Ca2+-dependent neurotransmitter release and presynaptic nerve terminal protein phosphorylation by calmodulin and a calmodulin-like protein isolated from synaptic vesicles
Synaptic vesicles have a Ca2+-dependent protein kinase system that may play a role in mediating Ca2+-stimulated neurotransmitter release and vesicle function. Calcium's ability to initiate norepinephrine release and protein phosphorylation in synaptic vesicle preparations was shown to be stimulated by the presence of an endogenous heat-stable vesicle protein
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10. A protein factor that inhibits ATP-dependent glutamate and γ-aminobutyric acid accumulation into synaptic vesicles: Purification and initial characterization
Glutamate, the major excitatory neurotransmitter in the mammalian central nervous system, is transported into and stored in synaptic vesicles. We have purified to apparent homogeneity a protein from brain cytosol that inhibits glutamate and γ-aminobutyric acid uptake into synaptic vesicles and have termed this protein “inhibitory protein factor” (IPF).
The National Academy of Sciences of the USA.
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11. Norepinephrine neuronal uptake binding sites in rat brain membranes labeled with [3H]desipramine.
Neuronal uptake recognition sites in norepinephrine neurons have been labeled with the antidepressant [3H]desipramine. A high-affinity component of [3H]desipramine binding to rat cerebral cortex membranes is abolished selectively by 6-hydroxydopamine lesions, which destroy central catecholamine neurons. The high-affinity [3H]desipramine binding has a nanomol
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12. Net uptake of gamma-aminobutyric acid by a high-affinity system of rat brain synaptosomes.
Rat brain synaptosomes isolated on discontinuous Ficoll gradient carry out rapid net uptake of gamma-aminobutyric acid through a high-affinity system (Km = 6.25 microM; Vmax = 1.2 nmol/min per mg of protein). The uptake of the labeled neurotransmitter is dependent on sodium concentration and is abolished by addition of 40 microM veratridine or 0.5 mM 2,4-dia