Ndna
Mostrando 13-24 de 30 artigos, teses e dissertações.
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13. Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death.
Programmed cell death (pcd) is activated during the hypersensitive response (HR) of plants to avirulent pathogens. We have recently shown that, similar to pcd in animal cells, nuclei of cells undergoing HR cell death contain fragmented nuclear DNA (nDNA). Here, we report that cell death occurring during the HR is accompanied by an increase in the activity of
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14. Evaluation of an ELISA system for determination of class-specific antibodies to native and denatured DNA in man.
Enzyme-linked immunosorbent assays (ELISA) have been set up for determination of plasma IgG and IgM antibodies to native (n) and denatured (d) DNA. Normal male and female donors generally gave low values in the assays for IgG; IgM control values were higher, particularly in females. Mean values for patients with systemic lupus erythematosus (SLE) were greatl
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15. Global Population Genetic Structure and Male-Mediated Gene Flow in the Green Turtle (Chelonia Mydas): RFLP Analyses of Anonymous Nuclear Loci
We introduce an approach for the analysis of Mendelian polymorphisms in nuclear DNA (nDNA), using restriction fragment patterns from anonymous single-copy regions amplified by the polymerase chain reaction, and apply this method to the elucidation of population structure and gene flow in the endangered green turtle, Chelonia mydas. Seven anonymous clones iso
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16. In Situ Detection of nDNA Fragmentation during the Differentiation of Tracheary Elements in Higher Plants.
Programmed cell death (pcd) is thought to occur during the autolysis of xylem vessels. Although several ultrastructural aspects of this differentiation process have been characterized, certain key aspects of this process remain unsolved. Here we demonstrate in pea (Pisum sativum) that nuclei of vessel elements undergoing pcd contain fragmented nDNA. This fin
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17. Disease activity in systemic lupus erythematosus related to a range of antibodies binding DNA and synthetic polynucleotides.
Antibodies to dDNA, nDNA, Z-DNA, poly(dT), poly(I), poly(dG.dC), poly(dA.dT), and total IgG and IgM were measured in five serial bleeds from 39 patients with systemic lupus erythematosus (SLE). The main findings were that those patients with renal disease form a distinct subset whose antibody levels correlate well with disease activity; anti-poly(dT) antibod
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18. Lupus autoantibodies to native DNA cross-react with the A and D SnRNP polypeptides.
Antibodies to native DNA (nDNA) in sera from patients with systemic lupus erythematosus have been found to frequently correlate with antibodies to the A and D SnRNP proteins measured in Western blot assays. 40 of 54 SLE (74.1%) sera with anti-nDNA bound to A and D proteins, while 9 of 113 sera (8%) without anti-nDNA bound the A and D proteins, P < 10(-8) by
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19. Novel molecular markers for late phases of the growth cycle of Arabidopsis thaliana cell-suspension cultures are expressed during organ senescence.
In an Arabidopsis thaliana T87-C3 cell-suspension culture, entry into the growth-arrest phase is rapidly followed by a loss of cell viability. Three cDNA clones, SRG1, SRG2, and SRG3, corresponding to genes with transcripts that accumulate during these late phases, were isolated by the mRNA differential display method. Amino acid sequence analysis shows that
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20. Manifold expression of new cellular genes in human lymphoid neoplasia.
Previous studies performed in our laboratory have shown that a minor single-stranded DNA component (ssDNA) isolated from the bulk of double-stranded nuclear DNA (nDNA) of human, murine, or avian cells consisted mainly of active transcription sites. In the present work, ssDNA isolated from human lymphoid cells, either malignant or not, amounted to 1.2-1.4% of
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21. Gibberellin Produced in the Cotyledon Is Required for Cell Division during Tissue Reunion in the Cortex of Cut Cucumber and Tomato Hypocotyls1
Cucumber (Cucumis sativus) hypocotyls were cut to one-half of their diameter transversely, and morphological and histochemical analyses of the process of tissue reunion in the cortex were performed. Cell division in the cortex commenced 3 d after cutting, and the cortex was nearly fully united within 7 d. 4′,6-Diamidino-2-phenylindole staining and 5-bromo-
American Society of Plant Physiologists.
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22. The Occurrence of Single-Stranded DNA in the Serum of Patients with Systemic Lupus Erythematosus and Other Diseases
Single-stranded DNA (SDNA) occurs in high incidence and in greatest concentration in the sera of patients with systemic lupus erythematosus (SLE), where levels as high as 250 μg/ml were observed. SDNA appears to be an imunogen for anti-SDNA antibodies and forms complexes in vivo of both anti-SDNA-SDNA and anti-NDNA-SDNA types, which apparently play a role i
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23. Evaluation of a standardised assay for the measurement of antibodies to double-stranded (native) DNA.
A standardised commercially available radioimmunoassay kit for the detection of antibodies to native DNA (N-DNA) has been evaluated in clinical practice. This test system is shown to be a reliably reproducible method of detecting these antibodies. In addition, evaluation of the purity of the radiolabelled test antigen in this assay has shown it to be almost
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24. Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs.
Comparison of plant mitochondrial (mt), chloroplast (cp) and nuclear (n) DNA sequences shows that the silent substitution rate in mtDNA is less than one-third that in cpDNA, which in turn evolves only half as fast as plant nDNA. The slower rate in mtDNA than in cpDNA is probably due to a lower mutation rate. Silent substitution rates in plant and mammalian m