Nanomolar Detection
Mostrando 1-12 de 19 artigos, teses e dissertações.
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1. Development of a Novel and Simple Electroanalytical Procedure for the Determination of Copper in Biofuel Employing a Sensor Based on Vulcan Functionalized with Carbazone
A novel and simple electroanalytical method for the determination of Cu2+ in biodiesel samples by stripping voltammetric analysis was developed. The method employs a carbon paste electrode (CPE) modified with Vulcan carbon, previouly functionalized with carbazone (CBZ). The CPE/Vulcan-CBZ sensor promoted a significant increase in the analytical signal obtain
J. Braz. Chem. Soc.. Publicado em: 2018-04
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2. Immobilization and sensing ability of human thyroid nuclear receptor in nanostructured thin films / Estudo da imobilização do receptor tireoidiano humano TRβ1 em filmes finos nanoestruturados e aplicações em detecção de hormônios tireoidianos
Manipulation of materials at the nanoscale represents one of the frontiers in nanoscience and nanotechnology, mainly due to the possibility of specific controlling, improved properties, not observed if conventional bulk processing is applied. For biomolecules, in particular, processing via immobilization in the form of nanostructured films has allowed their
Publicado em: 2010
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3. Caracterização da atividade antitumoral das tiossemicarbazonas derivadas de N(4)-Metil-Toluil-2-acetilpiridina e de 2-piridinoformamida e seus complexos metálicos: avaliação do potencial radiofarmaceutico / CHARACTERIZATION OF THE ANTITUMURAL ACTIVITY OF THE THIOSEMICARBAZONES DERIVED FROM N(4)-METHYL-TOLYL-2- ACETYLPYRIDINE AND 2-PIRIDINOFORMAMIDE AND ITS METAL COMPLEX: EVALUATION OF THE RADIOPHARMACEUTICAL POTENTIAL.
Thiosemicarbazones have attracted great pharmacological interest because of their biological properties, such as cytotoxic activity against multiple strains of human tumors. The most studied compounds are pyridine-based because of their resemblance to pyridoxal metabolites that attach to co-enzyme B6-dependant enzymes. This work aimed the characterization of
Publicado em: 2008
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4. Estudos bioeletroquímicos e eletroanalíticos da diospirina : agente leishmanicida e antitumoral
Analysis of molecular constituents based on natural products has acquired an important perspective in view of the renewed interest in the consumption of herbal prescriptions all over the world. Natural quinonoid compounds possess significant pharmacological properties and are responsible for the bioactivities of several plants. They showed several bioactivit
Publicado em: 2006
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5. Desenvolvimento de eletrodos ion-seletivos : aplicação em sistema de detecção em FIA e estrategias para a melhoria do limite de detecção / Development of ion-selective electrodes: application in detection system of FIA and strategies for the improvement of detection limit
The present work describes the development of ion-selective electrodes for calcium, potassium, sodium and chloride to be employed in flow injection analyses and its application for the simultaneous determination in samples, as well as, the development of solid contact ionselective electrodes with detection limit in the nanomolar range. The preparation of ion
Publicado em: 2005
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6. Imaging the nanomolar range of nitric oxide with an amplifier-coupled fluorescent indicator in living cells
Nitric oxide (NO) is a small uncharged free radical that is involved in diverse physiological and pathophysiological mechanisms. NO is generated by three isoforms of NO synthase, endothelial, neuronal, and inducible ones. When generated in vascular endothelial cells, NO plays a key role in vascular tone regulation, in particular. Here, we describe an amplifi
National Academy of Sciences.
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7. Degradation of Chlorobenzenes at Nanomolar Concentrations by Burkholderia sp. Strain PS14 in Liquid Cultures and in Soil
The utilization of 1,2,4,5-tetrachloro-, 1,2,4-trichloro-, the three isomeric dichlorobenzenes and fructose as the sole carbon and energy sources at nanomolar concentrations was studied in batch experiments with Burkholderia sp. strain PS14. In liquid culture, all chlorobenzenes were metabolized within 1 h from their initial concentration of 500 nM to below
American Society for Microbiology.
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8. Electronic detection of DNA by its intrinsic molecular charge
We report the selective and real-time detection of label-free DNA using an electronic readout. Microfabricated silicon field-effect sensors were used to directly monitor the increase in surface charge when DNA hybridizes on the sensor surface. The electrostatic immobilization of probe DNA on a positively charged poly-l-lysine layer allows hybridization at lo
National Academy of Sciences.
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9. Fluorophore Binding Aptamers as a Tool for RNA Visualization
Fluorescence correlation spectroscopy (FCS) is suitable for the detection of fluorescent molecules in living cells. For the visualization of mRNA, we genetically fused a fluorophore-specific RNA aptamer to the coding mRNA of the green fluorescent protein, as well as to noncoding sequences. Using these constructs, we showed that the aptamer portion of the mRN
The Biophysical Society.
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10. Cell-density-dependent sensitivity of a mer-lux bioassay.
The sensitivity of a previously described assay (O. Selifonova, R. Burlage, and T. Barkay, Appl. Environ. Microbiol. 59:3083-3090, 1993) for the detection of bioavailable inorganic mercury (Hg2+) by the activation of a mer-lux fusion was increased from nanomolar to picomolar concentrations by reducing biomass in the assays from 10(7) to 10(5) cells ml-1. The
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11. Quantitative Detection of Small Molecule/DNA Complexes Employing a Force-Based and Label-Free DNA-Microarray
Force-based ligand detection is a promising method to characterize molecular complexes label-free at physiological conditions. Because conventional implementations of this technique, e.g., based on atomic force microscopy or optical traps, are low-throughput and require extremely sensitive and sophisticated equipment, this approach has to date found only lim
The Biophysical Society.
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12. Detection of HIV-1 RNA by nucleic acid sequence-based amplification combined with fluorescence correlation spectroscopy
Nucleic acid sequence-based amplification (NASBA) has proved to be an ultrasensitive method for HIV-1 diagnosis in plasma even in the primary HIV infection stage. This technique was combined with fluorescence correlation spectroscopy (FCS) which enables online detection of the HIV-1 RNA molecules amplified by NASBA. A fluorescently labeled DNA probe at
The National Academy of Sciences of the USA.