Na H Exchanger
Mostrando 13-24 de 197 artigos, teses e dissertações.
-
13. Action of ANP on the nongenomic effects of aldosterone on the Na+/H+ exchanger in the S3 segment of proximal tubule of rat: studies in isolated tubules role of cytosolic calcium. / Ação do ANP no efeito não genômico da aldosterona sobre o trocador Na+/H+ no segmento S3 do túbulo proximal de rato - Estudos em túbulos isolados: função do cálcio citosólico.
The effects of aldosterone and ANP(2 min preincubation) on the intracellular pH recovery rate (pHirr) after the acid load induced by NH4Cl and on the [Ca2+]i were investigated in isolated rat S3 segment. The basal pHi was 7.20 + ou - 0.009(n=47/209) and the basal pHirr via the Na+/H+ exchanger was 0.195 + ou - 0.012 pHi/min(n=16/96). Aldosterone(10-12M) caus
Publicado em: 2008
-
14. UTILIZATION OF CHO CELLS CULTIVATED IN PRESENCE OF CYCLOHEXIMIDE FOR OBTAINMENT AND CHARACTARIZATION OF RECOMBINANT HUMAN GLYCOSYLATED PROLACTIN (G-hPRL) / Utilização de células CHO cultivadas na presença de cicloheximida para obtenção e caracterização de prolactina humana glicosilada (G-hPRL) recombinante
Human prolactin hPRL is a 199 aminoacid protein hormone (MM ~23.000 Da) with a wide spectrum of biological activities being, however, best known for its stimulation of lactation and development of mammary gland. Besides proteolytic cleavage, the majority of prolactin variants can be the result of other posttranslational processing of the mature molecule in t
Publicado em: 2008
-
15. Papel da proteína quinase C (PKC) na modulação da isoforma 1 do permutador Na+ - H+ (NHE1), em células MDCK. / Role of PKC on exchanger isoform 1 (NHE1), modulation in MDCK cells.
O presente trabalho visa contribuir para o esclarecimento da seqüência de eventos intracelulares produzidos pelas PKCs a e e, na modulação do pHi, via NHE1. Os estudos foram realizados em células MDCK e as medidas de pHi efetuadas por microscopia de fluorescência. A expressão das PKCs a e e, bem como do NHE1 foi investigada por western blot, utilizand
Publicado em: 2008
-
16. Efeito genômico e não-genômico da aldosterona no trocador Na+/H+ e na H+ - ATPase no túbulo proximal (S3): papel do cálcio citosólico. / Genomic and Nongenomic Effects of Aldosterone on Na+/H+ Exchanger and H+-ATPase in Proximal Tubule (S3): role of Cytosolic Calcium.
The present study indicate that the basal pHi of proximal S3 segment is 7.10 ? 0.007 (n = 444/2117), being made the extrusion by of Na+/H+ exchanger (mainly) and H+-ATPase. Our results suggest a role for cell calcium in regulating the process of pHi recovery after the acid load induced by NH4Cl, mostly mediated by a basolateral Na+/H+ exchanger, and stimulat
Publicado em: 2007
-
17. SYNTHESIS AND CHARACTERIZATION OF INORGANIC ION EXCHANGERS BASED ON MIXED OXIDE TIN-TITANIUM TO BE USED IN RECOVERY OF CADMIUM AND NICKEL AND PHOTOLUMINESCENT STUDIES / Síntese e caracterização de trocadores iônicos inorgânicos a base de óxidos mistos Estanho-Titânio para utilização na recuperação de Cádmio e Níquel e estudos fotoluminescentes
This work presents the synthesis, characterization and adsorption studies of inorganic ion exchangers based on mixed tin-titanium oxide for recovery of cadmium and nickel metals from aqueous effluents, discarded in the environment mainly through Ni-Cd battery. The exchangers were synthesized by sol-gel modified method using a mixture of tin(IV) chloride and
Publicado em: 2007
-
18. Leptospirose letal aguda em Hamster: caracterização de perfis bioquímicos, histopatológicos e celulares renais, relacionada a ensaios terapêuticos / Reversal of renal tubule transporter down-regulation during severe leptospirosis with antimicrobial therapy
Leptospirosis is a zoonosis of worldwide distribution. About 5-10% of all human infections presents with severe forms. Weils syndrome, the most common presentation of severe forms of leptospirosis, may courses either as a single monophasic disease or as a disease with prolonged course, characterized by a combination of hemorrhage, particularly in the lung, r
Publicado em: 2007
-
19. Estudo comparativo da pasteurização de leite pelo metodo convencional e por microondas. / Comparative study of milk pasteurization by convencional method and by microwaves.
O leite é um dos alimentos mais completos da natureza devido ao seu elevado valor nutritivo, como riqueza de proteínas, gordura e vitaminas; por esta mesma razão o leite é um excelente substrato para uma grande gama de microorganismos. A pasteurização visa eliminar os microorganismos patogênicos, e reduzir ao máximo a flora banal; no entanto os méto
Publicado em: 2007
-
20. Cinética de inativação térmica da peroxidase e da polifenoloxidase presentes na água de coco verde por processo térmico contínuo. / Kinetic of thermal inactivation of peroxidase and polyphenol oxidase present in green coconut water by thermal continuous processing.
Considering the need for green coconut water pasteurization after opening the fruit due to microbial and enzymatic (peroxidase (POD) and polyphenol oxidase (PFD)) action, the objective of this work is to study the behavior of the activity of these enzymes during continuous thermal processing. The first stage of the work was the study of the enzymes thermal i
Publicado em: 2005
-
21. Alterações moleculares no gene da banda 3 (AEI) na esferocitose hereditaria e a participação da isoforma renal no equilibrio acido-basico
A esferocitose é um tipo comum de anemia hemolítica hereditária cujo primeiro defeito molecular foi caracterizado em 1992. A proteína banda 3, codificada pelo gene AE1, foi a primeira isoforma clonada de uma famma de genes de trocadores de ânions "Anion Exchanger-AE gene family" e é a mais abundante das proteínas integrais da membrana eritrocitária.
Publicado em: 2000
-
22. Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.
A cDNA clone encoding a rabbit ileal villus cell Na+/H+ exchanger was isolated and its complete nucleotide sequence was determined. The cDNA is 4 kb long and contains 322 bp of 5'-untranslated region, 2451 bp of open reading frame and 1163 bp of 3'-untranslated area, with 70%, 91% and 40% identity to the human sequence, respectively. Amino acid sequence dedu
-
23. Functional characteristics of a cloned epithelial Na+/H+ exchanger (NHE3): resistance to amiloride and inhibition by protein kinase C.
We previously cloned an isoform Na+/H+ exchanger (NHE3), which was expressed only in intestine, kidney, and stomach. We show here the functional characteristics of NHE3 as a Na+/H+ exchanger by stably transfecting NHE3 cDNA into PS120 cells, a fibroblast cell line that lacks endogenous Na+/H+ exchangers. NHE3 was 39- and 160-fold more resistant to inhibition
-
24. Molecular cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger (NHE-2).
The present studies demonstrate cloning, sequencing, tissue distribution, and functional expression of a Na+/H+ exchanger which was isolated from a rat intestinal cDNA library. The cloned cDNA recognizes two transcripts in poly(A)+ RNA from the stomach, jejunum, ileum, liver, large intestine, and uterus. Based on deduced amino acid sequences, this clone shar