Myosins
Mostrando 1-12 de 121 artigos, teses e dissertações.
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1. Investigações estruturais dos domínios funcionais das miosinas classes VIII e XI presentes em plantas / Structural investigations of the functional domains of plant myosins (classes VIII and XI)
As miosinas formam uma superfamília de proteínas de alto peso molecular com atividade mecanoquímica capaz de hidrolisar a molécula de ATP e de interagir com os filamentos de actina. A estrutura das miosinas pode ser divida de modo geral em cabeça motora, pescoço e cauda. São conhecidas 35 classes de miosinas em eucariotos sendo a classe II de miosinas
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/02/2012
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2. Isolamento e caracterizaÃÃo parcial dos Genes beta-actina e miosina de cadeia pesada do CamarÃo rosa Farfantepenaeus subtilis / Isolation and partial characterization of genes beta-actin and myosin heavy chain shrimp Farfantepenaeus subtilis
The penaeid shrimp Farfantepenaeus subtilis is an important native species for fisheries industry in Brazil. Among marine shrimps of commercial importance, penaeids are recognized as a valuable resource for fishery and aquaculture in tropical and subtropical regions. However, data on these species is extremely reduced, especially concerning genetic elements
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/03/2009
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3. Autorreatividade humoral a peptídeos da miosina cardíaca e proteína de choque térmico 60: estudo sequencial em pacientes transplantados cardíacos e indivíduos sadios / Humoral autoreactivity to peptides from cardiac myosin and heat shock protein 60: sequential study in heart transplanted patients and healthy subjects
The immune response directed to self antigens can contribute to the pathogenesis of autoimmune diseases. However, autoimmunity may also have an immunoregulatory role in allograft rejection and in other inflammatory processes. We analyzed IgG and IgM autoantibodies reactive to peptides from the human cardiac myosin (CM) and the heat shock protein 60 (Hsp60) i
Publicado em: 2009
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4. Analyse der Funktion der nichtmuskulären schweren Myosinketten in glatten Muskelzellen
Das Ziel dieser Studie war es, die Beteiligung der nichtmuskulären schweren Myosinketten an der Kontraktion der glatten Muskeln unter physiologischen Bedingungen zu untersuchen. Als Versuchsmodell wurde die Harnblase von neugeborenen Wildtyp und transgenen Mäusen verwendet, bei denen das Gen für die glattmuskelspezifischen schweren Myosinketten durch Gene
Publicado em: 2003
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5. Myosin V and iNOS expression is enhanced in J774 murine macrophages treated with IFN-gamma
Actin-based motor protein requirements and nitric oxide (NO) production are important features of macrophage activity during phagocytosis or microbicidal processes. Different classes of myosins contribute directly or indirectly to phagocytosis by providing mechanical force for phagosome closure or organelle movement. Recent data have shown the presence of my
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-02
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6. Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types.
Myosin diversity in the human epithelial cell line Caco-2BBe, the porcine epithelial cell line LLC-PK1 (CL-4), human peripheral blood leukocytes, and human liver was analyzed. PCR amplification yielded 8-11 putative myosins (depending on the cDNA source) representing six distinct myosin classes. Analysis of clones obtained by hybridization screening demonstr
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7. Transport of Myosin II to the Equatorial Region without Its Own Motor Activity in Mitotic Dictyostelium Cells
Fluorescently labeled myosin moved and accumulated circumferentially in the equatorial region of dividing Dictyostelium cells within a time course of 4 min, followed by contraction of the contractile ring. To investigate the mechanism of this transport process, we have expressed three mutant myosins that cannot hydrolyze ATP in myosin null cells. Immunofluor
The American Society for Cell Biology.
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8. p21-activated kinase has substrate specificity similar to Acanthamoeba myosin I heavy chain kinase and activates Acanthamoeba myosin I
Acanthamoeba class I myosins are unconventional, single-headed myosins that express actin-activated Mg2+-ATPase and in vitro motility activities only when a single serine or threonine in the heavy chain is phosphorylated by myosin I heavy chain kinase (MIHCK). Some other, but not most, class I myosins have the same consensus phosphorylation site sequence, an
The National Academy of Sciences of the USA.
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9. A Millennial Myosin Census
The past decade has seen a remarkable explosion in our knowledge of the size and diversity of the myosin superfamily. Since these actin-based motors are candidates to provide the molecular basis for many cellular movements, it is essential that motility researchers be aware of the complete set of myosins in a given organism. The availability of cDNA and
The American Society for Cell Biology.
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10. Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum.
The 2116-amino acid myosin heavy chain sequence from Dictyostelium discoideum was determined from DNA sequence analysis of the cloned gene. The gene product can be divided into two distinct regions, a globular head region and a long alpha-helical, rod-like tail. In comparisons with nematode and mammalian muscle myosins, specific areas of the head region are
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11. Differences among myosins synthesized in non-myogenic cells, presumptive myoblasts, and myoblasts.
Myosins synthesized in non-myogenic cells and replicating presumptive myoblasts differ from those synthesized in postmitotic mononucleated myoblasts and myotubes. Myoblasts and myotubes synthesize the definitive light chains, MLC1 and MLC2. These light chains display different molecular weights in sodium dodecyl sulfate-polyacrylamide gels from the fibroblas
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12. In vitro actin filament sliding velocities produced by mixtures of different types of myosin.
Using in vitro motility assays, we examined the sliding velocity of actin filaments generated by pairwise mixings of six different types of actively cycling myosins. In isolation, the six myosins translocated actin filaments at differing velocities. We found that only small proportions of a more slowly translating myosin type could significantly inhibit the