Mycoplasma Hyorhinis
Mostrando 13-24 de 73 artigos, teses e dissertações.
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13. Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.
A 13-kilobase DNA fragment from a genomic library of Mycoplasma hyorhinis demonstrated specific Southern hybridization and dot hybridization when tested against a group of different mycoplasmas. This probe selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differenti
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14. Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis.
Mycoplasma hyorhinis, isolated by isopycnic centrifugation from supernatants of a persistently infected murine T lymphoblastoid cell line, demonstrated the presence of the Thy-1.1 differentiation alloantigen and H-2Kk histocompatibility antigens. The murine leukemia virus-related gp70 antigen also present on the surface of these lymphoblastoid cells was abse
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15. Antigenic mimicry of a human cellular polypeptide by Mycoplasma hyorhinis.
A 46-kilodalton (kDa) polypeptide was immunoprecipitated from radiolabeled extracts of human cell lines infected with Mycoplasma hyorhinis by murine monoclonal antibodies PF/2A and ML77. Both of these antibodies also reacted in an enzyme-linked immunosorbent assay (ELISA) with M. hyorhinis cells and with human and nonhuman cell lines infected with M. hyorhin
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16. Macrolide susceptibility of Mycoplasma hyorhinis isolated from piglets.
Twenty strains of Mycoplasma hyorhinis were investigated for their in vitro susceptibilities to 15 antimicrobial agents by broth and agar dilution methods. Two of the 20 field strains showed low susceptibility to 14- and 16-membered macrolide antimicrobial agents tested. The two field strains were considered inducibly resistant to macrolides.
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17. In Vitro Susceptibility of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis to Fifty-One Antimicrobial Agents
By a tube dilution assay technique, 51 antimicrobial agents were singly tested against 9 strains of Mycoplasma hyopneumoniae and 1 strain of M. hyorhinis for the purpose of obtaining information useful for selecting an agent for testing in vivo against porcine mycoplasmal pneumonia. Based on determining minimal inhibitory concentrations and chemically groupi
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18. Mycoplasma hyorhinis Swine Arthritis III. Modified Disease in Piney Woods Swine
Piney Woods miniature swine, when injected with Mycoplasma hyorhinis, develop an acute arthritis. The disease in these animals is limited clinically, microbiologically, and pathologically to a mild acute illness without the more chronic and destructive features noted in a previous study of the experimental disease in Yorkshire swine.
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19. Infection by Mycoplasma hyorhinis strongly enhances uptake of antisense oligonucleotides: a reassessment of receptor-mediated endocytosis in the HepG2 cell line
This paper shows that the ∼66 kDa band, previously isolated from the HepG2 cell line as an oligonucleotide (ON) plasma membrane ‘receptor’, is induced by Mycoplasma infection. Moreover, this band has been identified as the invariant membrane protein of Mycoplasma hyorhinis, p70, based on ribosomal DNA sequencing combined with ON ligand blotting after p
Oxford University Press.
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20. Comparison of methods for in vitro testing of susceptibility of porcine Mycoplasma species to antimicrobial agents.
The MICs of 18 antimicrobial agents used against strains of three porcine Mycoplasma species were determined by a serial broth dilution method. Twenty field strains of M. hyorhinis, ten field strains of M. hyopneumoniae, six field strains of M. flocculare, and the type strains of these species were tested. Twelve field strains and the type strain of M. hyorh
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21. Isolation of folded chromosomes from Mycoplasma hyorhinis.
Folded chromosomes were isolated from Mycoplasma hyorhinis. When examined by electron microscopy, these molecules show variability of loop size, number of loops, total contour length and degree of twisting of the DNA. Sedimentation velocity was unaltered after treatment with RNase, proteinase K, SDS, temperatures up to 65 degrees C and NaCl concentrations fr
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22. Methylated bases in mycoplasmal DNA.
The DNAs of four Mycoplasma and one Acholeplasma species were found to contain methylated bases. All of the five species contained 6-methyladenine (m6Ade), the methylated base characteristic of prokaryotic DNA. The extent of methylation of adenine residues in the mycoplasmal DNA ranged from 0.2% in Mycoplasma capricolum to about 2% in Mycoplasma arginini and
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23. Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically.
Mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. Since both mitochondria and mycoplasmas synthesize DNA by using the prokaryotic DNA polymerase gamma, the use of aphidicolin, which inhibits eukaryotic DNA polymerase alpha, allows for selective synthesis of mycoplasmal and mitochondrial DNA. The restri
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24. Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy.
The chromosome of Mycoplasma hyorhinis was analyzed by using different restriction endonucleases and electron microscopy. It was found that restriction enzymes BstEII, XhoI, and SacI are the enzymes of choice for analysis and characterization of M. hyorhinis. The bands resulting from digestion of M. hyorhinis DNA with BstEII had apparent molecular weights ra