Mutagen Testing
Mostrando 1-9 de 9 artigos, teses e dissertações.
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1. Avaliação da mutagenicidade e antimutagenicidade de extratos aquosos e orgânicos do Agaricus blazei Murill (Cogumelo do Sol ) linhagem AB97/11, in vitro
Existem várias espécies de cogumelos, sendo que muitas delas são utilizadas na medicina e na alimentação. O Agaricus blazei Murrill é uma espécie nativa do Brasil, muito consumida devido a suas propriedades medicinais. No presente trabalho a avaliação da atividade mutagênica e antimutagênica de extratos aquosos da linhagem AB97/11, na fase jovem e
Publicado em: 2002
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2. Simplified Method for Testing Mutagens in Saccharomyces
Pretreatment of yeast auxotrophs with mutagen on complete medium stimulates the reversion frequency.
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3. unc-93(e1500): A Behavioral Mutant of CAENORHABDITIS ELEGANS That Defines a Gene with a Wild-Type Null Phenotype
The uncoordinated, egg-laying-defective mutation, unc-93(e1500) III, of the nematode Caenorhabditis elegans spontaneously reverts to a wild-type phenotype. We describe 102 spontaneous and mutagen-induced revertants that define three loci, two extragenic (sup-9 II and sup-10 X) and one intragenic. Genetic analysis suggests that e1500 is a rare visible allele
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4. Mass isolation and fertility testing of temperature-sensitive mutants in Tetrahymena.
A set of 239 heat-sensitive (38 degrees) and 16 cold-sensitive (18 degrees) conditional mutants of Tetrahymena has been generated by combining techniques to manipulate large numbers of clones with a method for the selection of self-fertilized cells after mutagen treatment. A simple technique is presented for determining the fertility of individual clones; 17
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5. A Class of Population Genetic Questions Formulated as the Generalized Occupancy Problem
In categorical genetic data analysis when the sampling units are classified into an arbitrary number of distinct classes, sometimes the sample size may not be large enough to apply large sample approximations for hypothesis testing purposes. Exact sampling distributions of several statistics are derived here, using combinatorial approaches parallel to the cl
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6. A mutagen-testing assay based on heterogeneity in diameter and integrated optical density of mammalian cell colonies.
We investigated the effects of the well-known mutagenic agents ethyl methanesulfonate (EtMes), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), and ICR-191 on colonies of the Chinese hamster ovary line CHO cultured on a semisolid substrate. These agents induced heterogeneity in diameter and integrated optical density of colonies as determined by computer-assisted
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7. Activation of cycasin to a mutagen for Saccharomyces cerevisiae by rat intestinal flora.
Genetic test systems involving microorganisms and liver enzyme preparations may be insufficient to detect compounds that require breakdown by enzymes provided by the microbial flora of the intestinal tract. A method is described for providing such activation and for simultaneously testing the potential genetic activity of breakdown products in an indicator o
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8. Mutagenicity testing of some commonly used dyes.
Seventeen commonly used dyes and 16 of their metabolites or derivatives were tested in the Salmonella-mammalian microsome mutagenicity test. Mutagens active with and without added Aroclor-induced rat liver microsome preparations (S9) were 3-aminopyrene, lithol red, methylene blue (USP), methyl yellow, neutral red, and phenol red. Those mutagenic only with S9
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9. Base pair conformation-dependent excision of benzo[a]pyrene diol epoxide-guanine adducts by human nucleotide excision repair enzymes.
Human nucleotide excision repair processes carcinogen-DNA adducts at highly variable rates, even at adjacent sites along individual genes. Here, we identify conformational determinants of fast or slow repair by testing excision of N2-guanine adducts formed by benzo[a]pyrene diol epoxide (BPDE), a potent and ubiquitous mutagen that induces mainly G x C-->T x