Mpss
Mostrando 13-22 de 22 artigos, teses e dissertações.
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13. Statistical analysis of MPSS measurements: Application to the study of LPS-activated macrophage gene expression
Massively Parallel Signature Sequencing (MPSS), a recently developed high-throughput transcription profiling technology, has the ability to profile almost every transcript in a sample without requiring prior knowledge of the sequence of the transcribed genes. As is the case with DNA microarrays, effective data analysis depends crucially on understanding how
National Academy of Sciences.
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14. Multiplex polymerase chain reaction (PCR) with color-tagged module-shuffling primers for comparing gene expression levels in various cells
A method based on the multiplex polymerase chain reaction (PCR) and gel electrophoresis for the comparative analysis of gene expression levels was developed. Using the method many cDNA fragments from different sources can be compared simultaneously. Competitive PCR amplification of expressed genes from different sources was performed by using ‘module-
Oxford University Press.
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15. An atlas of human gene expression from massively parallel signature sequencing (MPSS)
We have used massively parallel signature sequencing (MPSS) to sample the transcriptomes of 32 normal human tissues to an unprecedented depth, thus documenting the patterns of expression of almost 20,000 genes with high sensitivity and specificity. The data confirm the widely held belief that differences in gene expression between cell and tissue types are l
Cold Spring Harbor Laboratory Press.
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16. MPSS profiling of human embryonic stem cells
BioMed Central.
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17. Arabidopsis MPSS. An Online Resource for Quantitative Expression Analysis1[w]
American Society of Plant Biologists.
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18. The Use of MPSS for Whole-Genome Transcriptional Analysis in Arabidopsis
We have generated 36,991,173 17-base sequence “signatures” representing transcripts from the model plant Arabidopsis. These data were derived by massively parallel signature sequencing (MPSS) from 14 libraries and comprised 268,132 distinct sequences. Comparable data were also obtained with 20-base signatures. We developed a method for handling these dat
Cold Spring Harbor Laboratory Press.
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19. Phagocytosis Affects Biguanide Sensitivity of Acanthamoeba spp.
The incidence of Acanthamoeba keratitis, a disease associated with contact lens wear, has been in apparent decline with the advent of multipurpose contact lens solutions. The concentrations of the biguanides chlorhexidine digluconate (CHX) and particularly polyhexamethylene biguanide (PHMB) included in multipurpose solutions (MPSs) are sublethal for amoebae.
American Society for Microbiology.
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20. AMPDB: the Arabidopsis Mitochondrial Protein Database
The Arabidopsis Mitochondrial Protein Database is an Internet-accessible relational database containing information on the predicted and experimentally confirmed protein complement of mitochondria from the model plant Arabidopsis thaliana (http://www.ampdb.bcs.uwa.edu.au/). The database was formed using the total non-redundant nuclear and organelle encoded s
Oxford University Press.
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21. Comprehensive sampling of gene expression in human cell lines with massively parallel signature sequencing
Whereas information is rapidly accumulating about the structure and position of genes encoded in the human genome, less is known about the complexity and relative abundance of their expression in individual human cells and tissues. Here, we describe the characteristics of the transcriptomes of two cultured cell lines, HB4a (normal breast epithelium) and HCT-
The National Academy of Sciences.
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22. Preparation of functionally active cell-permeable peptides by single-step ligation of two peptide modules
Noninvasive cellular import of synthetic peptides can be accomplished by incorporating a hydrophobic, membrane-permeable sequence (MPS). Herein, we describe a facile method that expedites synthesis of biologically active, cell-permeable peptides by site-specific ligation of two free peptide modules: one bearing a functional sequence and the second bearing a
The National Academy of Sciences.