Microsporidia
Mostrando 13-24 de 98 artigos, teses e dissertações.
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13. Estudos de campo e de laboratorio sobre o parasitismo por microsporideos e mermitideos em populações larvais de simulideos
Foi realizado um levantamento de larvas de simulídeos em localidades dos estados de São Paulo, Paraná, Mato Grosso, Rio Grande do Sul, Santa Catarina e Minas Gerais, durante o período de fevereiro de 1996 a maio de 1998. Após a análise das larvas de simulídeos coletadas em cada localidade, encontrou-se parasitismo por Polydispyrenia simu/ii (Microspor
Publicado em: 1998
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14. Patologia e epizootiologia de Simulium pertinax (Diptera ; Simuliidae) infectado por Polydispyrenia simulii (Microspora ; Duboscquiidae) e Gastromermis viridis cf. (Nematoda ; Mermithidae)
No presente trabalho foram investigados vários aspectos da patologia e epizotiologia de dois agentes biológicos, o microsporídeo Polydispyrenia simulii (Microspora; Duboscquiidae) e o nematódeo Gastromermis viridis cf (Nematoda; Mermithidae), que ocorrem naturalmente no borrachudo Simulium pertinax (Dip.; Simuliidae) na região estudada. Esta espécie de
Publicado em: 1994
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15. Confirmation of the Human-Pathogenic Microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in Water
Microsporidia, as a group, cause a wide range of infections, though two species of microsporidia in particular, Enterocytozoon bieneusi and Encephalitozoon intestinalis, are associated with gastrointestinal disease in humans. To date, the mode of transmission and environmental occurrence of microsporidia have not been elucidated due to lack of sensitive and
American Society for Microbiology.
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16. In Vitro Cultivation of Microsporidia of Clinical Importance
Although attempts to develop methods for the in vitro cultivation of microsporidia began as early as 1937, the interest in the culture of these organisms was confined mostly to microsporidia that infect insects. The successful cultivation in 1969 of Encephalitozoon cuniculi, a microsporidium of mammalian origin, and the subsequent identification of these org
American Society for Microbiology.
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17. Detection of microsporidial spores in fecal specimens from patients diagnosed with cryptosporidiosis.
Patients infected with Cryptosporidium parvum may have concurrent infections with microsporidia. Two modified trichrome stains and a polyclonal indirect fluorescent-antibody procedure were used for the detection of microsporidia; the Merifluor Cryptosporidium-Giardia monoclonal direct immunofluorescence detection kit was used for the detection of C. parvum.
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18. Molecular Techniques for Detection, Species Differentiation, and Phylogenetic Analysis of Microsporidia
Microsporidia are obligate intracellular protozoan parasites that infect a broad range of vertebrates and invertebrates. These parasites are now recognized as one of the most common pathogens in human immunodeficiency virus-infected patients. For most patients with infectious diseases, microbiological isolation and identification techniques offer the most ra
American Society for Microbiology.
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19. Discrimination between Viable and Dead Encephalitozoon cuniculi (Microsporidian) Spores by Dual Staining with Sytox Green and Calcofluor White M2R
Microsporidia are obligate intracellular parasites, recognized as causing chronic diarrhea and systemic disease in AIDS patients, organ transplant recipients, travelers, and malnourished children. Species of microsporidia that infect humans have been detected in drinking-water sources, and methods are needed to ascertain if these microsporidia are viable and
American Society for Microbiology.
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20. Microsporidia are related to Fungi: Evidence from the largest subunit of RNA polymerase II and other proteins
We have determined complete gene sequences encoding the largest subunit of the RNA polymerase II (RBP1) from two Microsporidia, Vairimorpha necatrix and Nosema locustae. Phylogenetic analyses of these and other RPB1 sequences strongly support the notion that Microsporidia are not early-diverging eukaryotes but instead are specifically related to Fungi. Our r
The National Academy of Sciences.
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21. Human microsporidial infections.
Microsporidia are obligate intracellular spore-forming protozoal parasites belonging to the phylum Microspora. Their host range is extensive, including most invertebrates and all classes of vertebrates. More than 100 microsporidial genera and almost 1,000 species have now been identified. Five genera (Enterocytozoon spp., Encephalitozoon spp., Septata spp.,
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22. Evaluation of an Immunofluorescent-Antibody Test Using Monoclonal Antibodies Directed against Enterocytozoon bieneusi and Encephalitozoon intestinalis for Diagnosis of Intestinal Microsporidiosis in Bamako (Mali)
A 2-month study was carried out in Mali to evaluate an immunofluorescent-antibody test (IFAT) using monoclonal probes specific for Enterocytozoon bieneusi or Encephalitozoon intestinalis. Sixty-one human immunodeficiency virus (HIV)-seropositive adult patients and 71 immunocompetent children were enrolled. Microsporidia were detected in stools from 8 of 61 p
American Society for Microbiology.
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23. Detection of Microsporidia in Travelers with Diarrhea
We examined stool specimens of 148 returning travelers from an outpatient department for tropical diseases for the appearence of microsporidia using light microscopy and PCR. Intestinal microsporidiosis was diagnosed for five patients by light microscopy and for nine patients by PCR. Some cases were diagnosed only by PCR, indicating that the true prevalence
American Society for Microbiology.
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24. Comparison of three staining methods for detecting microsporidia in fluids.
Calcofluor white 2MR, modified trichrome blue, and indirect immunofluorescent antibody (IFA) staining methods were evaluated and compared for detecting microsporidia in stool. Serial 10-fold dilutions of Encephalitozoon (Septata) intestinalis were prepared in three formalinized stool specimens or in Tris-buffered saline. Ten-microliter aliquots were smeared