Microscopy Fluorescence Methods
Mostrando 13-24 de 124 artigos, teses e dissertações.
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13. Glutaraldehyde test for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis in an area with high tuberculosis incidence
BACKGROUND Tuberculosis (TB) remains one of the leading causes of morbidity and mortality worldwide. The primary method for controlling TB is the rapid and accurate identification of infected individuals. Immune response exploitation represents one of the main methods used for early TB diagnosis; however, few studies have reported that whole blood originati
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-11
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14. Comparative analysis of different doses of coherent light (laser) and non-coherent light (light-emitting diode) on cellular necrosis and apoptosis: a study in vitro
Introduction Threshold doses of electromagnetic radiation can initiate necrosis and apoptosis in cells. The purpose of this study was to evaluate cellular apoptosis and necrosis immediately (t0) and 24 hours (t24) after irradiation with different doses of coherent light (laser) or non-coherent light (LED). Methods CHO-K1 lineage cells were irradiated with
Res. Biomed. Eng.. Publicado em: 2016-12
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15. Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms
Abstract Aims We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance. Methods Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from patients with nosocomial infection in the surgical
Braz J Infect Dis. Publicado em: 2016-08
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16. New insights into the structural characteristics of irradiated crotamine
Abstract Background: Since ionizing radiation has the potential to alter the molecular structure and affect the biologica properties of biomolecules, it has been successfully employed to attenuate animal toxins. The present study aimed to characterize the structural modifications on irradiated crotamine, a toxin from
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/08/2015
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17. The effect of elemene on lung adenocarcinoma A549 cell radiosensitivity and elucidation of its mechanism
OBJECTIVE:To investigate the effect of elemene on the radiosensitivity of A549 cells and its possible molecular mechanism.METHODS:Apoptosis of A549 cells was detected by flow cytometry and fluorescence microscopy. The effect of double-strand break (DSB) damage repair in A549 cells was evaluated using the neutral comet assay. Protein expression levels were de
Clinics. Publicado em: 2015-08
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18. Automatic identification of tuberculosis mycobacterium
Introduction According to the Global TB control report of 2013, “Tuberculosis (TB) remains a major global health problem. In 2012, an estimated 8.6 million people developed TB and 1.3 million died from the disease. Two main sputum smear microscopy techniques are used for TB diagnosis: Fluorescence microscopy and conventional microscopy. Fluorescence micro
Res. Biomed. Eng.. Publicado em: 2015-03
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19. Different methods to quantify Listeria monocytogenesbiofilms cells showed different profile in their viability
Listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in food processing plants. The aim of this study was to evaluate the resistance of Listeria monocytogenes attached to abiotic surface, after treatment with sanitizers, by culture method, microscopy and Quantitative Real Time Polymerase Cha
Braz. J. Microbiol.. Publicado em: 2015-03
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20. Microbial mediated preparation, characterization and optimization of gold nanoparticles
The need for eco-friendly and cost effective methods for nanoparticles synthesis is developing interest in biological approaches which are free from the use of toxic chemicals as byproducts. This study aimed to biosynthesize and optimize the size of gold nanoparticles which produced by biotechnological method using Penicillium crustosum isolated from soil. I
Braz. J. Microbiol.. Publicado em: 2014-12
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21. Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites
Objective: To study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells. Methods: Lactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentr
J. Appl. Oral Sci.. Publicado em: 2014-07
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22. Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
PURPOSE:To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS:Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the
Acta Cir. Bras.. Publicado em: 2014
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23. Impacto da presenÃa de atrazina na comunidade bacteriana do solo / Impact of atrazine on bacteriological soil community
Chemical contamination removal in soil and water depends on microbiological community that is able to degrade these compounds. There is a great evolutionary interest on studying microorganisms that metabolize the xenobiotic ones, since they have relatively been seen as new in the last five decades. Little is known about structure variation of microbiological
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/02/2012
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24. Dinâmica de crescimento de Cedrela odorata L. (Meliaceae) na Floresta Atlântica do Estado do Rio de Janeiro: fenologia, atividade cambial e dendrocronologia / Growth dynamics of Cedrela Odorata L. (Meliaceae) on the Atlantic Rain Forest of the State of Rio de Janeiro: phenology, cambial activity and dendrochronology
Along the 20th century, a few dendrochronological studies were developed with tropical species, according to the belief that variations of the climatic conditions on those regions werent regular and strong enough to induce an annual rate of radial growth. The researches on this topic in the last decades showed that the formation of annual growth rings in the
Publicado em: 2011