Microsatellite Repeats
Mostrando 25-36 de 128 artigos, teses e dissertações.
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25. Application of a double-enrichment procedure for microsatellite isolation and the use of tailed primers for high throughput genotyping
The number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic
Genetics and Molecular Biology. Publicado em: 2007-03
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26. Isolamento e caracterização de marcadores microssatelites para a mosca-dos-chifres, Haematobia irritans (Linnaeus, 1758) / Isolation and characterization of polymorphic microsatelite loci for the horn fly, Haematobia irritans (Linnaeus, 1758)
The aim of this work was the construction of a genomic microsatelliteenriched library for the species Haematobia irritans irritans (Diptera: Muscidae), commonly known as orn fly an ectoparasite of great economic importance world-wide, and particularly in Brazil. Here we describe ten polymorphic microsatellite loci isolated from this species. From a complete
Publicado em: 2007
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27. Aplicação de microssatélites (STR) de bovinos em animais de raças zebuínas
O gado zebu (Bos indicus) é predominante em todo o mundo. Atualmente, no Brasil, o rebanho de bovinos e zebuínos é composto por aproximadamente 170 milhões de indivíduos, no qual, 80% são zebuínos ou animais de raças cruzadas com zebu. O principal objetivo deste estudo foi determinar a possibilidade da utilização de marcadores moleculares de bovino
Publicado em: 2007
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28. Development and characterization of polymorphic microsatellite markers for the sweet orange (Citrus sinensis L. Osbeck)
The aims of this study were to develop simple sequence repeat markers (SSRs or microsatellite markers) in citrus and to evaluate the efficiency of these markers for characterization of sweet orange. We developed SSRs from a genomic library of 'Pêra IAC' sweet orange enriched for AG/TC, GT/CA, TCA/AGT and AAC/TTG sequence repeats. We selected 279 sequences f
Genetics and Molecular Biology. Publicado em: 2006
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29. Origin, evolution and genome distribution of microsatellites
Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-
Genetics and Molecular Biology. Publicado em: 2006
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30. Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)
The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and
Genetics and Molecular Biology. Publicado em: 2006
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31. "Estudo das alterações dos microssatélites D6S251 e D6S252 no carcinoma basocelular esporádico" / Study of alterations in microsatellites D6S251 and D6S252 in sporadic basal cell carcinoma
Existe grande interesse na determinação das bases genéticas do carcinoma basocelular (CBC) que expliquem seu fenótipo pouco agressivo e comportamento metastático infreqüente. Investigamos a instabilidade de microssatélites (MSI) e perda de heterozigosidade (LOH) nos microssatélites D6S251 (6q14) e D6S252 (6q16) de CBCs esporádicos de alto e baixo ri
Publicado em: 2006
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32. Variabilidade genetica e estrutura de populações de Cochliomyia hominivorax (Diptera: Calliphoridae) : uma nova perspectiva atraves de marcadores microssatelites / Genetic diversity and population structure in the new world screw-worm, Cochliomyia hominivorax (Diptera: Calliphoridae), as revealed by microsatellite markers
The cultural and economic importance of animal production in South America dates back to the colonial era. This continent has one of the largest percentages of the world s total cattle population and countries like Brazil, Argentina and Uruguay play a major role as exporters of a variety of livestock products. Diseases affecting livestock can have a signific
Publicado em: 2006
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33. Estudo de marcadores polimórficos da região 7q11.23 para o diagnóstico da síndrome de Williams-Beuren / Williams-Beuren syndrome: molecular diagnoses using polimorphic markers to 7q11.23 region
INTRODUÇÃO: A síndrome de Williams-Beuren (SWB) resulta de uma deleção de aproximadamente 1.5 Mb na região 7q11.23. A haploinsuficiência ocasiona alterações do desenvolvimento neurológico assim como malformações em múltiplos sistemas. OBJETIVOS: Testar utilidade de marcadores polimórficos para o diagnóstico da síndrome, determinar a proporç�
Publicado em: 2006
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34. Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)
The isolation of (GA)n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-ra
Genetics and Molecular Biology. Publicado em: 2003
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35. Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers
Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty hig
Genetics and Molecular Biology. Publicado em: 2003
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36. Characterization of new Schistosoma mansoni microsatellite loci in sequences obtained from public DNA databases and microsatellite enriched genomic libraries
In the last decade microsatellites have become one of the most useful genetic markers used in a large number of organisms due to their abundance and high level of polymorphism. Microsatellites have been used for individual identification, paternity tests, forensic studies and population genetics. Data on microsatellite abundance comes preferentially from mic
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-10