Microfluidics
Mostrando 13-24 de 26 artigos, teses e dissertações.
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13. Development of microfluidics as endothelial progenitor cell capture technology for cardiovascular tissue engineering and diagnostic medicine
We have developed a unique microfluidic platform capable of capturing circulating endothelial progenitor cells (EPCs) by understanding surface chemistries and adhesion profiles. The surface of a variable-shear-stress microfluidic device was conjugated with 6 different antibodies [anti-CD34, -CD31, -vascular endothelial growth factor receptor-2 (VEGFR-2), -CD
The Federation of American Societies for Experimental Biology.
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14. A bubble-driven microfluidic transport element for bioengineering
Microfluidics typically uses channels to transport small objects by actuation forces such as an applied pressure difference or thermocapillarity. We propose that acoustic streaming is an alternative means of directional transport at small scales. Microbubbles on a substrate establish well controlled fluid motion on very small scales; combinations (“doublet
National Academy of Sciences.
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15. Influence of Internal Capsid Pressure on Viral Infection by Phage λ
Ejection of the genome from the virus, phage λ, is the initial step in the infection of its host bacterium. In vitro, the ejection depends sensitively on internal pressure within the virus capsid; however, the in vivo effect of internal pressure on infection of bacteria is unknown. Here, we use microfluidics to monitor individual cells and determine the t
The Biophysical Society.
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16. Using three-dimensional microfluidic networks for solving computationally hard problems
This paper describes the design of a parallel algorithm that uses moving fluids in a three-dimensional microfluidic system to solve a nondeterministically polynomial complete problem (the maximal clique problem) in polynomial time. This algorithm relies on (i) parallel fabrication of the microfluidic system, (ii) parallel searching of all potential solu
The National Academy of Sciences.
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17. Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy
We review the current state of multiphoton microscopy. In particular, the requirements and limitations associated with high-speed multiphoton imaging are considered. A description of the different scanning technologies such as line scan, multifoci approaches, multidepth microscopy, and novel detection techniques is given. The main nonlinear optical contrast
American Institute of Physics.
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18. Wetting morphologies at microstructured surfaces
The wetting of microstructured surfaces is studied both experimentally and theoretically. Even relatively simple surface topographies such as grooves with rectangular cross section exhibit a large variety of different wetting morphologies as observed by atomic force microscopy. This polymorphism arises from liquid wedge formation along the groove corners and
National Academy of Sciences.
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19. Fabrication of aligned microstructures with a single elastomeric stamp
The fabrication of complex patterns of aligned microstructures has required the use of multiple applications of lithography. Here we describe an approach for microfabrication that encodes the two-dimensional spatial information of several photomasks onto a single elastomeric stamp by mapping each photomask onto distinct heights on the surface of the stamp. P
The National Academy of Sciences.
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20. Microfluidics without microfabrication
Microfluidic devices create spatially defined, chemically controlled environments at microscopic dimensions. We demonstrate the formation and control of microscopic hydrodynamic and chemical environments by impinging a low-intensity acoustic oscillation on a cylindrical electrode. The interaction of small-amplitude (≤203 μm), low-frequency (≤515 Hz) flu
The National Academy of Sciences.
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21. Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences
Large DNA constructs of arbitrary sequences can currently be assembled with relative ease by joining short synthetic oligodeoxynucleotides (oligonucleotides). The ability to mass produce these synthetic genes readily will have a significant impact on research in biology and medicine. Presently, high-throughput gene synthesis is unlikely, due to the limits of
Oxford University Press.
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22. Microbial DNA Typing by Automated Repetitive-Sequence-Based PCR
Repetitive sequence-based PCR (rep-PCR) has been recognized as an effective method for bacterial strain typing. Recently, rep-PCR has been commercially adapted to an automated format known as the DiversiLab system to provide a reliable PCR-based typing system for clinical laboratories. We describe the adaptations made to automate rep-PCR and explore the perf
American Society for Microbiology.
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23. Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA–protein conjugates
An assay for very sensitive antigen detection is described which takes advantage of the self- assembly capabilities of semi-synthetic conjugates of DNA and proteins. The general scheme of this assay is similar to a two-sided (sandwich) enzyme-linked immunoassay (ELISA); however, covalent single-stranded DNA–streptavidin (STV) conjugates, capable of hybridi
Oxford University Press.
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24. Computerized microfluidic cell culture using elastomeric channels and Braille displays
Computer-controlled microfluidics would advance many types of cellular assays and microscale tissue engineering studies wherever spatiotemporal changes in fluidics need to be defined. However, this goal has been elusive because of the limited availability of integrated, programmable pumps and valves. This paper demonstrates how a refreshable Braille display,
National Academy of Sciences.