Microfilaments
Mostrando 13-24 de 278 artigos, teses e dissertações.
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13. Pattern formation and handedness in the cytoskeleton of human platelets.
The cytoskeletal patterns of human platelets spread on a glass surface are analyzed. F-actin is arranged in patterns of parallel microfilaments, microfilaments forming triangles, or microfilaments radiating tangentially from a central ellipse or circle. Vinculin, a cytoskeletal protein, is located at both ends of the filaments. In platelets with tangentially
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14. Actin in the Green Alga, Nitella
Bundles of microfilaments very similar in appearance to actin are present in cytoplasmic suspensions obtained from Nitella flexilis. The microfilaments bind rabbit heavy meromyosin in arrowhead arrays similar to those produced on muscle actin. The arrowheads are removed with ATP. The results provide evidence that actin is present in green plants, probably in
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15. Actin in Dividing Cells: Contractile Ring Filaments Bind Heavy Meromyosin
Many microfilaments and microtubules are well preserved after glycerol-extraction of HeLa cells at room temperature (22°). Incubation in heavy meromyosin from rabbit skeletal muscle results in conspicuous and characteristic “decoration” of microfilaments of the contractile ring. Decoration is completely prevented by 10 mM ATP or 2 mM pyrophosphate, and
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16. A Plant-Specific Kinesin Binds to Actin Microfilaments and Interacts with Cortical Microtubules in Cotton Fibers1
A novel kinesin, GhKCH1, has been identified from cotton (Gossypium hirsutum) fibers. GhKCH1 has a centrally located kinesin catalytic core, a signature neck peptide of minus end-directed kinesins, and a unique calponin homology (CH) domain at its N terminus. GhKCH1 and other CH domain-containing kinesins (KCHs) belong to a distinct branch of the minus end-d
American Society of Plant Biologists.
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17. Interferon increases the abundance of submembranous microfilaments in HeLa-S3 cells in suspension culture.
Human beta (fibroblast) interferon inhibits the proliferation of human HeLa-S3 carcinoma cells in suspension culture. Accompanying this effect, the lateral mobility of cell surface receptors for concanavalin A is decreased and the rigidity of the plasma membrane lipid bilayer is increased. The present findings show a marked increase in the number of polymeri
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18. Effects of Cytochalasin B upon Microfilaments Involved in Morphogenesis of Salivary Epithelium
Cytochalasin B causes cultured mouse salivary gland epithelium to lose its characteristic shape and to cease undergoing morphogenesis. The drug causes disorganization of the 50 Å microfilaments in epithelial cells that are thought to control cell shape because of contractile properties. Upon removal of cytochalasin, epithelia regain normal shape and resume
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19. Sequential rearrangement and nuclear polymerization of actin in baculovirus-infected Spodoptera frugiperda cells.
Proper assembly of nucleocapsids of the baculovirus Autographa californica nuclear polyhedrosis virus is prevented by cytochalasin D, a drug that interferes with actin microfilament function. To investigate the involvement of microfilaments in A. californica nuclear polyhedrosis virus replication, a fluorescence microscopy study was conducted that correlated
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20. Cytochalasin B: Effects upon Microfilaments Involved in Morphogenesis of Estrogen-Induced Glands of Oviduct*
The administration of estrogen to immature chicks induces formation of tubular glands and differentiation of cells in the oviduct. As glands begin to form, organized bundles of 40-50 Å filaments appear at the luminal end of the cells. These structures are not present in uninduced oviducts. Cytochalasin treatment of oviducts early in gland formation results
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21. Polarization of Specific Tropomyosin Isoforms in Gastrointestinal Epithelial Cells and Their Impact on CFTR at the Apical Surface
Microfilaments have been reported to be polarized in a number of cell types based both on function and isoform composition. There is evidence that microfilaments are involved in the movement of vesicles and the polarized delivery of proteins to specialized membrane domains. We have investigated the composition of actin microfilaments in gastrointestinal epit
The American Society for Cell Biology.
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22. Membrane Microfilaments of Erythrocytes: Alteration in Intact Cells Reproduces the Hereditary Spherocytosis Syndrome
Membrane microfilaments are found throughout the animal world in situations suggesting that they fulfill a critical role in providing normal cell shape and plasticity. We have hypothesized that hereditary spherocytosis, a congenital hemolytic anemia associated with intrinsically rigid and mishapen erythrocytes, might result from genetically defective microfi
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23. 3-Deazaadenosine-induced disorganization of macrophage microfilaments.
3-Deazaadenosine (c3Ado) has been reported to inhibit a number of cellular functions. These biological effects of c3Ado have generally been attributed to its ability to act as inhibitor and substrate of S-adenosylhomocysteine hydrolase. In this report, it is revealed by fluorescence microscopy that c3Ado caused disorganization of the microfilament system of
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24. Involvement of Actin Microfilaments in the Replication of Human Parainfluenza Virus Type 3
Several studies indicate that paramyxoviruses require a specific cellular factor(s) for transcription of their genomic RNAs. We previously reported that the cellular cytoskeletal protein actin, in its polymeric form, participates in the transcription of human parainfluenza virus type 3 (HPIV3) in vitro. In the present study, we investigated the role of the p
American Society for Microbiology.