Microcarriers
Mostrando 1-12 de 15 artigos, teses e dissertações.
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1. Desenvolvimento de um bioprocesso para expansão de células mesenquimais estromais multipotentes em microcarregadores / Bioprocess development for expansion of mesenchymal stem cells on microcarriers.
As células mesenquimais estromais multipotentes (CMM) são na atualidade uma fonte atrativa para aplicações na engenharia de tecidos e na terapia celular. Devido à baixa disponibilidade nos tecidos (0,01%-0,0005%) e às elevadas doses necessárias para uma infusão (aproximadamente 106 células/Kg paciente) tornou-se necessário o desenvolvimento de tecn
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/05/2012
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2. Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up
Brazilian Journal of Medical and Biological Research. Publicado em: 2009-06
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3. Influence of culture conditions on Vero cell propagation on non-porous microcarriers
Cultivos de células animais são amplamente utilizados para a produção de vacinas virais e para a expressão de proteínas recombinantes. A linhagem celular Vero é uma linhagem contínua, dependente de ancoragem, recomendada pela Organização Mundial de Saúde para a produção de vacinas de uso humano. Para a produção de vacinas virais em larga escal
Brazilian Archives of Biology and Technology. Publicado em: 2005-06
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4. Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells
The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by s
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-02
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5. Growth of Fish Cell Lines on Microcarriers
Microcarrier beads were evaluated as substrates for the propagation of five anchorage-dependent fish cell lines. Growth of rainbow trout gonad (RTG-2) and Atlantic salmon cells was limited on microcarriers maintained in suspension. However, stationary microcarriers were suitable substrates for the growth of RTG-2, AS, Chinook salmon embryo (CHSE-214), and fa
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6. Survival, organization, and function of microcarrier-attached hepatocytes transplanted in rats.
Hepatocytes harvested by collagenase perfusion of rat liver were attached to collagen-coated microcarriers and injected intraperitoneally into congeneic or allogeneic bilirubin-UDP-glucuronosyltransferase (EC 2.4.1.17)-deficient (Gunn) rats or allogeneic analbuminemic (NAR) rats. Five days later, the microcarriers were observed to have formed conglomerates c
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7. Mycoplasma gallisepticum in culture with biosilon microcarrier beads.
Various dilutions of Mycoplasma gallisepticum were cultured in the presence of Biosilon microcarrier beads. The microcarriers did not affect the recoverability or the growth rate of M. gallisepticum. Cultures attained a higher density in the presence of microcarriers. The initiation of a culture could be accomplished by the transfer of one bead from a microc
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8. Growth of Vero E-6 cells on microcarriers in a cell bioreactor.
Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated polystyrene (Kontes Life Sciences Products
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9. Microcarriers in combination with enzyme immunofiltration and immunofluroescence for the detection of herpes simplex virus antigens in culture.
A microcarrier culture system in combination with enzyme immunofiltration with a herpes simplex virus (HSV) group monoclonal antibody was found to be as sensitive as immunofluorescence for the detection of HSV type 1 (HSV-1) in cell cultures and to give specific identification at the same time as the appearance of the cytopathic effect with very high infecti
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10. Changes in cytosolic Ca2+ associated with von Willebrand factor release in human endothelial cells exposed to histamine. Study of microcarrier cell monolayers using the fluorescent probe indo-1.
A method for measuring fluorescence in anchored monolayers of human endothelial cells is described and used to demonstrate changes in the cytosolic free-calcium concentration ([Ca2+]c) in these cells exposed to histamine and thrombin; some endothelial responses to both agonists (e.g., mitogenesis) have been suggested to be Ca2+-mediated. Umbilical vein endot
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11. Adult fast myosin pattern and Ca2+-induced slow myosin pattern in primary skeletal muscle culture
A primary muscle cell culture derived from newborn rabbit muscle and growing on microcarriers in suspension was established. When cultured for several weeks, the myotubes in this model develop the completely adult pattern of fast myosin light and heavy chains. When Ca2+ ionophore is added to the culture medium on day 11, raising intracellular [Ca2+] about 10
The National Academy of Sciences of the USA.
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12. New method of hepatocyte transplantation and extracorporeal liver support.
A technique has been developed by the authors that allows hepatocyte attachment on collagen-coated microcarriers resulting in prolonged hepatocyte viability and function both in vivo and in vitro. Rat hepatocytes were obtained by portal vein collagenase perfusion. Intraperitoneally transplanted microcarrier-attached normal hepatocytes into congeneic Gunn rat