Methylomonas Sp
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Denitrification coupled with methane anoxic oxidation and microbial community involved identification
In this work, the biological denitrification associated with anoxic oxidation of methane and the microbial diversity involved were studied. Kinetic tests for nitrate (NO3-) and nitrite (NO2-) removal and methane uptake were carried out in 100 mL batch reactors incubated in a shaker (40 rpm) at 30 ºC. Denitrificant/methanotrophic biomass was taken from a lab
Brazilian Archives of Biology and Technology. Publicado em: 2011-02
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2. Utilização do metano como doador de elétrons para remoção de nitrogênio via nitrificação e desnitrificação em reator operado em bateladas seqüenciais / Nitrogen removal by biological nitrification and denitrification using methane as electron donor in a sequencing batch aerobic/anoxic reactor
Nitrogen removal via biological nitrification and denitrification was studied in a sequencing batch reactor submitted to aerate and anoxic periods. Methane was added as electron donor for denitrification in the anoxic periods. Different operational and nutritional conditions referred to as stages in this text were tested aiming to achieve the best nitrogen r
Publicado em: 2008
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3. Localization of methanol dehydrogenase in two strains of methylotrophic bacteria detected by immunogold labeling.
Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of the methylotroph Methylobacterium sp. strai
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4. Methanol oxidation genes in the marine methanotroph Methylomonas sp. strain A4.
Methanol dehydrogenase has been purified from the type I marine methanotroph Methylomonas sp. strain A4 and found to be similar to other methanol dehydrogenase enzymes in subunit composition, molecular mass, and N-terminal sequence of the two subunits. A heterologous gene probe and a homologous oligonucleotide have been used to identify a DNA fragment from M
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5. Soluble Methane Monooxygenase Gene Clusters from Trichloroethylene-Degrading Methylomonas sp. Strains and Detection of Methanotrophs during In Situ Bioremediation
The soluble MMO (sMMO) gene clusters from group I methanotrophs were characterized. An 8.1-kb KpnI fragment from Methylomonas sp. strain KSWIII and a 7.5-kb SalI fragment from Methylomonas sp. strain KSPIII which contained the sMMO gene clusters were cloned and sequenced. The sequences of these two fragments were almost identical. The sMMO gene clusters in t
American Society for Microbiology.
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6. Inhibition of trichloroethylene oxidation by the transformation intermediate carbon monoxide.
Inhibition of trichloroethylene (TCE) oxidation by the transformation intermediate carbon monoxide (CO) was evaluated with the aquifer methanotroph Methylomonas sp. strain MM2. CO was a TCE transformation intermediate. During TCE oxidation, approximately 9 mol% of the TCE was transformed to CO. CO was oxidized by Methylomonas sp. strain MM2, and when formate
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7. Soluble cytochromes from the marine methanotroph Methylomonas sp. strain A4.
Soluble c-type cytochromes are central to metabolism of C1 compounds in methylotrophic bacteria. In order to characterize the role of c-type cytochromes in methane-utilizing bacteria (methanotrophs), we have purified four different cytochromes, cytochromes c-554, c-553, c-552, and c-551, from the marine methanotroph Methylomonas sp. strain A4. The two major
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8. Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer.
Trichloroethylene (TCE)-transforming aquifer methanotrophs were evaluated for the influence of TCE oxidation toxicity and the effect of reductant availability on TCE transformation rates during methane starvation. TCE oxidation at relatively low (6 mg liter-1) TCE concentrations significantly reduced subsequent methane utilization in mixed and pure cultures
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9. Identification of putative methanol dehydrogenase (moxF) structural genes in methylotrophs and cloning of moxF genes from Methylococcus capsulatus bath and Methylomonas albus BG8.
An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putative moxF genes from two obligate methanotr
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10. Cytochrome P460 Genes from the Methanotroph Methylococcus capsulatus Bath†
P460 cytochromes catalyze the oxidation of hydroxylamine to nitrite. They have been isolated from the ammonia-oxidizing bacterium Nitrosomonas europaea (R. H. Erickson and A. B. Hooper, Biochim. Biophys. Acta 275:231–244, 1972) and the methane-oxidizing bacterium Methylococcus capsulatus Bath (J. A. Zahn et al., J. Bacteriol. 176:5879–5887, 1994). A dege
American Society for Microbiology.