Mechanical Inspection
Mostrando 25-25 de 25 artigos, teses e dissertações.
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25. Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli.
MscL is a channel that opens a large pore in the Escherichia coli cytoplasmic membrane in response to mechanical stress. Previously, we highly enriched the MscL protein by using patch clamp as a functional assay and cloned the corresponding gene. The predicted protein contains a largely hydrophobic core spanning two-thirds of the molecule and a more hydrophi